Rationale: Cell-free protein microarrays display naturally-folded proteins based on just-in-time in situ synthesis, and have made important contributions to basic and translational research. However, the risk of spot-to-spot cross-talk from protein diffusion during expression has limited the feature density of these arrays.
Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot.
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- Digital object identifier: 10.7150/thno.20151
- Identifier TypeInternational standard serial numberIdentifier Value1309-1042
- Copyright Ivyspring International Publisher
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Yu, X., Song, L., Petritis, B., Bian, X., Wang, H., Viloria, J., . . . Labaer, J. (2017). Multiplexed Nucleic Acid Programmable Protein Arrays. Theranostics, 7(16), 4057-4070. doi:10.7150/thno.20151