Rationale: Cell-free protein microarrays display naturally-folded proteins based on just-in-time in situ synthesis, and have made important contributions to basic and translational research. However, the risk of spot-to-spot cross-talk from protein diffusion during expression has limited the feature density of these arrays.
Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot.
Download count: 0
Details
- Yu, Xiaobo (Author)
- Song, Lusheng (Contributor)
- Petritis, Brianne (Contributor)
- Bian, Xiaofang (Contributor)
- Wang, Haoyu (Contributor)
- Viloria, Jennifer (Contributor)
- Park, Jin (Contributor)
- Bui, Hoang (Contributor)
- Li, Han (Contributor)
- Wang, Jie (Contributor)
- Liu, Lei (Author)
- Yang, Liuhui (Author)
- Duan, Hu (Author)
- McMurray, David N. (Author)
- Achkar, Jacqueline M. (Author)
- Magee, Mitch (Contributor)
- Qiu, Ji (Contributor)
- LaBaer, Joshua (Contributor)
- Biodesign Institute (Contributor)
- 2017-09-20
- Digital object identifier: 10.7150/thno.20151
- Identifier TypeInternational standard serial numberIdentifier Value1309-1042
- Copyright Ivyspring International Publisher
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Yu, X., Song, L., Petritis, B., Bian, X., Wang, H., Viloria, J., . . . Labaer, J. (2017). Multiplexed Nucleic Acid Programmable Protein Arrays. Theranostics, 7(16), 4057-4070. doi:10.7150/thno.20151