Full metadata
Title
Toxicity study in Alzheimer's disease cell model
Description
Alzheimer's disease (AD) is the most common type of dementia, affecting one in nine people age 65 and older. One of the most important neuropathological characteristics of Alzheimer's disease is the aggregation and deposition of the protein beta-amyloid. Beta-amyloid is produced by proteolytic processing of the Amyloid Precursor Protein (APP). Production of beta-amyloid from APP is increased when cells are subject to stress since both APP and beta-secretase are upregulated by stress. An increased beta-amyloid level promotes aggregation of beta-amyloid into toxic species which cause an increase in reactive oxygen species (ROS) and a decrease in cell viability. Therefore reducing beta-amyloid generation is a promising method to control cell damage following stress. The goal of this thesis was to test the effect of inhibiting beta-amyloid production inside stressed AD cell model. Hydrogen peroxide was used as stressing agent. Two treatments were used to inhibit beta-amyloid production, including iBSec1, an scFv designed to block beta-secretase site of APP, and DIA10D, a bispecific tandem scFv engineered to cleave alpha-secretase site of APP and block beta-secretase site of APP. iBSec1 treatment was added extracellularly while DIA10D was stably expressed inside cell using PSECTAG vector. Increase in reactive oxygen species and decrease in cell viability were observed after addition of hydrogen peroxide to AD cell model. The increase in stress induced toxicity caused by addition of hydrogen peroxide was dramatically decreased by simultaneously treating the cells with iBSec1 or DIA10D to block the increase in beta-amyloid levels resulting from the upregulation of APP and beta-secretase.
Date Created
2014
Contributors
- Suryadi, Vicky (Author)
- Sierks, Michael (Thesis advisor)
- Nielsen, David (Committee member)
- Dai, Lenore (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
viii, 45 p. : ill. (some col.)
Language
eng
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.24847
Statement of Responsibility
by Vicky Suryadi
Description Source
Viewed on July 8, 2014
Level of coding
full
Note
thesis
Partial requirement for: M.S., Arizona State University, 2014
bibliography
Includes bibliographical references (p. 40-45)
Field of study: Chemical engineering
System Created
- 2014-06-09 02:08:09
System Modified
- 2021-08-30 01:35:43
- 3 years 3 months ago
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