Caregiving for a disabled or ill relative can be an extremely stressful role, leading to increased acute physiological responses to stress and also chronic cardiovascular disease (CVD) outcomes. Furthermore, an important consideration for studying CVD and daily stress in caregivers is less is known about gender differences in the effects of caregiving. The aims of this study were to examine the associations between gender and daily stressors on cardiovascular health in caregivers. A sample of 1,841 respondents (ranging from 33-84 years) from the Midlife in the United States study answered a question about caregiving and participated in both a daily diary and biomarker subprojects. Within the sample, only 13.04% of respondents classified themselves as caregivers. Regression analysis was used to test 1. whether stressor exposure and stressor reactivity, which is the emotional response to stress, differ by caregiver status and gender, 2. cardiovascular health as indicated by the American Heart Association’s Life’s Simple Seven measure (on scale of poor, intermediate, or ideal) is predicted by caregiving status and gender, 3. whether daily stress is associated with Life’s Simple Seven, and 4. whether daily stress moderates the associations between caregiving and gender with cardiovascular health. Important findings of the study included that caregivers and women experienced more daily stressors, while caregivers and men had greater cardiovascular risk, but this was not related to experience of daily stressors. These results are discussed within the context of study limitations and opportunities for future research building upon daily stress and caregiving.