Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement.
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- Nogly, Przemyslaw (Author)
- Panneels, Valerie (Author)
- Nelson, Garrett (Author)
- Gati, Cornelius (Author)
- Kimura, Tetsunari (Author)
- Milne, Christopher (Author)
- Milathianaki, Despina (Author)
- Kubo, Minoru (Author)
- Wu, Wenting (Author)
- Conrad, Chelsie (Author)
- Coe, Jesse (Author)
- Bean, Richard (Author)
- Zhao, Yun (Author)
- Bath, Petra (Author)
- Dods, Robert (Author)
- Harimoorthy, Rajiv (Author)
- Beyerlein, Kenneth R. (Author)
- Rheinberger, Jan (Author)
- James, Daniel (Author)
- Deponte, Daniel (Author)
- Li, Chufeng (Author)
- Sala, Leonardo (Author)
- Williams, Garth J. (Author)
- Hunter, Mark S. (Author)
- Koglin, Jason E. (Author)
- Berntsen, Peter (Author)
- Nango, Eriko (Author)
- Iwata, So (Author)
- Chapman, Henry N. (Author)
- Fromme, Petra (Author)
- Frank, Matthias (Author)
- Abela, Rafael (Author)
- Boutet, Sebastien (Author)
- Barty, Anton (Author)
- White, Thomas A. (Author)
- Weierstall, Uwe (Author)
- Spence, John (Author)
- Neutze, Richard (Author)
- Schertler, Gebhard (Author)
- Standfuss, Jorg (Author)
- College of Liberal Arts and Sciences (Contributor)
- Department of Physics (Contributor)
- Department of Chemistry and Biochemistry (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- School of Molecular Sciences (Contributor)
- Digital object identifier: 10.1038/ncomms12314
- Identifier TypeInternational standard serial numberIdentifier Value2041-1723
- The final version of this article, as published in Nature Communications, can be viewed online at: https://www.nature.com/articles/ncomms12314, opens in a new window
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Nogly, P., Panneels, V., Nelson, G., Gati, C., Kimura, T., Milne, C., . . . Standfuss, J. (2016). Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography. Nature Communications, 7, 12314. doi:10.1038/ncomms12314