CTB-MPR is a fusion protein between the B subunit of cholera toxin (CTB) and the membrane-proximal region of gp41 (MPR), the transmembrane envelope protein of Human immunodeficiency virus 1 (HIV-1), and has previously been shown to induce the production of anti-HIV-1 antibodies with antiviral functions. To further improve the design of this candidate vaccine, X-ray crystallography experiments were performed to obtain structural information about this fusion protein. Several variants of CTB-MPR were designed, constructed and recombinantly expressed in Escherichia coli.
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- Digital object identifier: 10.1107/S2052252514014900
- Identifier TypeInternational standard serial numberIdentifier Value2052-2525
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Lee, H., Cherni, I., Yu, H., Fromme, R., Doran, J. D., Grotjohann, I., . . . Mor, T. S. (2014). Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1. IUCrJ, 1(5), 305-317. doi:10.1107/s2052252514014900