Gut Microbiome of Subcutaneous Tumor Mouse Model Received Intratumor Injection of Engineered Salmonella Treatment

Description
This study investigates the impact of intratumor injection of genetically engineered Salmonella in a colorectal cancer (CRC) subcutaneous (SC) tumor mouse model. We aimed to assess the treatment’s safety and its effect on the gut microbiome. Using a cohort of

This study investigates the impact of intratumor injection of genetically engineered Salmonella in a colorectal cancer (CRC) subcutaneous (SC) tumor mouse model. We aimed to assess the treatment’s safety and its effect on the gut microbiome. Using a cohort of 50 adult mice divided into treatment (Salmonella) and control (PBS) groups, fecal samples were collected at three timepoints: pre-treatment (T0), 10 days post-treatment (T1), and 20 days post-treatment (T2). The nucleic acid extraction was completed on all of the samples. Through 16S rRNA amplicon sequencing, we observed no Salmonella in any samples, confirming the treatment’s microbiome safety. Alpha diversity revealed significant changes over time within groups, while beta diversity analysis showed clustering primarily by timepoint. Differential abundance analysis highlighted shifts in gut microbiota composition, potentially linked to treatment and cancer progression. These findings offer a preliminary understanding of the microbiome’s response to microbial cancer therapies and underscore the importance of further research to optimize safety and efficacy.

Details

Contributors
Date Created
2025-05
Embargo Release Date

Additional Information

English
Series
  • Academic Year 2024-2025
Extent
  • 27 pages
Open Access
Peer-reviewed

On Compactifications and Stone-\v{C}ech Theory

Description
This paper explores the Stone-Čech compactification as a versatile tool in topology, known for its ability to extend locally compact Hausdorff spaces to a maximal compact framework. Through a detailed analysis of its universal properties, this compactification is presented as

This paper explores the Stone-Čech compactification as a versatile tool in topology, known for its ability to extend locally compact Hausdorff spaces to a maximal compact framework. Through a detailed analysis of its universal properties, this compactification is presented as essential for understanding the boundary behavior of continuous functions and the broader structure of topological spaces. Key topics include the compactification’s role in mapping spaces through ultrafilters, its applications in functional analysis, algebra, and topological dynamics, and its connections to set theory and combinatorics. By examining the implications of the Stone-Čech boundary and its interaction with logical and algebraic structures, this work underscores the compactification’s profound influence in both theoretical and applied mathematical contexts, offering a foundation for future explorations in topology and related fields.

Details

Contributors
Date Created
2024-12
Resource Type

Additional Information

English
Series
  • Academic Year 2024-2025
Extent
  • 44 pages
Open Access
Peer-reviewed

Examining the Relation Between Baseline Anxiety and Depression and Subjective Response Following Cannabis Flower Consumption and the Moderating Role of THC Potency

Description
Subjective response to a drug is an important variable in understanding addiction, yet it has received very little attention in cannabis use. Given that mental health concerns are a commonly cited reason for cannabis use, perhaps people experiencing affective disturbances

Subjective response to a drug is an important variable in understanding addiction, yet it has received very little attention in cannabis use. Given that mental health concerns are a commonly cited reason for cannabis use, perhaps people experiencing affective disturbances such as anxiety and depression may experience more rewarding subjective responses. Additionally, due to the growing influx of THC in cannabis products, which was found to produce positive and negative subjective responses, this study investigated the moderating role of THC potency between anxiety, depression, and subjective response. We used ecological momentary assessment data from 105 regular cannabis-using participants. We used hierarchical multiple regression to examine baseline anxiety and depression scores and THC levels as predictors of subjective response (generally positive, low arousal negative, and high arousal negative) to cannabis flower (main effect), as well as interactions between baseline anxiety and depression and THC concentration (interactions). We found that at higher THC concentrations, higher levels of anxiety and depression were associated with an increase in positive subjective response. Likewise, higher levels of depression were associated with more high arousal negative effects. Future research should investigate THC levels across different cannabis products and include broader measures to capture THC levels in the body.

Details

Contributors
Date Created
2024-12
Embargo Release Date

Additional Information

English
Series
  • Academic Year 2024-2025
Extent
  • 43 pages
Open Access
Peer-reviewed

Asian/Asian Pacific American Students' Coalition (AAPASC) 2024-2025 Guidebook

Description
By assembling the countless work put in by past and current AAPASC community members, this guidebook aims to serve as a foundation for future AAPASC executive boards and organizations. The goal for this project is to be a living document,

By assembling the countless work put in by past and current AAPASC community members, this guidebook aims to serve as a foundation for future AAPASC executive boards and organizations. The goal for this project is to be a living document, maintained and changed by future generations of AAPI student leadership to adapt to the needs and goals of their university experience, but also to provide a connection to the learned experience of the past.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 69 pages
Open Access
Peer-reviewed

Building an ESG Metrics Database for US Law Firms: ESG Intelligence Group's Data Management Solution

Description
I built a database for ESG Intelligence (ESGi) Group, a consulting firm that advises law firms on implementing good environmental, social, and governance (ESG) practices internally as well as how law firms can better serve their clients with respect to

I built a database for ESG Intelligence (ESGi) Group, a consulting firm that advises law firms on implementing good environmental, social, and governance (ESG) practices internally as well as how law firms can better serve their clients with respect to ESG. This paper explores my decision-making process for the design of the database and the challenges I ran into while creating and populating the database. I turned a list of things that ESGi Group wanted to track into an entity relationship diagram (ERD), which I eventually turned into a relational database in MySQL. I further defined the contents of the database by mapping the ERD into a relational model, normalizing the relational model, and creating an attribute domain table. I coded the database in SQL, collected data in an excel spreadsheet (downloaded from AMLAW 200 and NLJ 500 purchased data, manually searching individual firm websites, and scraping law.com in R), and then inserted the data into the database. I ran into issues with data completeness due to the lacking regulation of firm transparency about ESG reporting, but this project succeeded in proof of concept rather than implementation. I also discuss security and privacy considerations, and ESGi Group’s possible options for further development of this project in the future.

Details

Contributors
Date Created
2024-05
Topical Subject
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 28 pages
Open Access
Peer-reviewed

Enhancing Youth Agency in Urban Communities through Environmental Justice Workshops

Description
Historically, young people have spearheaded environmental movements, demanding equitable involvement in decision-making processes that impact their future. Despite their active participation, barriers such as inadequate knowledge, lack of empowerment, and diminished hope often hinder meaningful engagement and impact. This study

Historically, young people have spearheaded environmental movements, demanding equitable involvement in decision-making processes that impact their future. Despite their active participation, barriers such as inadequate knowledge, lack of empowerment, and diminished hope often hinder meaningful engagement and impact. This study addresses these challenges by implementing a series of educational workshops designed to equip youth with the necessary tools to effectively influence climate policy and urban planning so that they can feel more hopeful about the future in the face of climate change. Utilizing both qualitative and quantitative methods, this research evaluates how different methods of arts-based educational engagement impact workshop participants' knowledge, empowerment, and optimism regarding their ability to inspire environmental change. The findings aim to contribute to the discourse on effective youth engagement in environmental justice, advocating for strategies that equip youth with the tools they need to foster sustainable community development and hope for the future.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 38 pages
Open Access
Peer-reviewed

Automated Transcription of Greek Manuscripts

Description
The automated transcription of Greek manuscripts is a current research goal in the digital humanities. Pre-processing manuscript images is an important part of any computer based transcription pipeline. However, pre-processing for ancient manuscripts specifically has not been highly developed. The

The automated transcription of Greek manuscripts is a current research goal in the digital humanities. Pre-processing manuscript images is an important part of any computer based transcription pipeline. However, pre-processing for ancient manuscripts specifically has not been highly developed. The result of this project is a noiseless pre-processing method that keeps diacritics. Further, text line segmentation is automated for manuscripts without annotation.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 64 pages
Open Access
Peer-reviewed

Improving Succinate Production in E. coli through Substrate Channeling

Description
Current industrial production of petrochemicals releases CO2 as a byproduct into the atmosphere, contributing to climate change. The sustainable alternative, microbial carbon capture, has primarily focused on phototrophs that have naturally occurring carbon fixation pathways, but are slow-growing, difficult to genetically engineer, and

Current industrial production of petrochemicals releases CO2 as a byproduct into the atmosphere, contributing to climate change. The sustainable alternative, microbial carbon capture, has primarily focused on phototrophs that have naturally occurring carbon fixation pathways, but are slow-growing, difficult to genetically engineer, and require sunlight, which limits their large-scale production capacity. Using a heterotroph such as Escherichia coli allows for chemical production at high titers, rates, and yields (TRY) while being fast growing and easy to genetically engineer. Under fermentation conditions, the carboxylases in E. coli fix inorganic carbon in the reductive branch of the TCA cycle, producing industrially relevant chemical precursors such as succinate. However, the carboxylase’s access to CO2 is limited by the conditions surrounding it; most of the inorganic carbon inside the cell is in the form of bicarbonate. Increasing the local concentration of CO2 near the carboxylase may improve the kinetics of the pathway. To do this, a fusion protein that colocalizes carbonic anhydrase and phosphoenolpyruvate carboxykinase (Pck) was created. However, since strains expressing this fusion protein did not grow above OD600 = 1 under fermentation conditions, further design optimization and investigation is needed.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 15 pages
Open Access
Peer-reviewed

Women's Power and Influence Index: Survey Analysis of Employee Benefits

Description
The Women’s Power and Influence Index uses publicly available information to rank companies based on their gender policies, with the thought that public rankings trigger the behavioral and policy changes that move us in the direction of gender equity and

The Women’s Power and Influence Index uses publicly available information to rank companies based on their gender policies, with the thought that public rankings trigger the behavioral and policy changes that move us in the direction of gender equity and pay parity. This project employs survey analysis to take a closer look at four of the criteria the WPI uses to score companies - maternity leave, childcare, harassment and discrimination training, and professional development. Our work evaluates survey responses to determine optimal policies for each of the four criteria with the hope that in future iterations of the Index, these policies can be incorporated into the scoring methods as a standard against which respective company policies can be compared.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 33 pages
Open Access
Peer-reviewed

An Evaluation of Convergent Case Management

Description
Convergent Case Management (CCM) is a mandatory reentry program offered in Arizona state-run prison units. This study evaluates the success of this program through semi-structured interviews with correctional officers and incarcerated men and women at two Arizona prison units. These

Convergent Case Management (CCM) is a mandatory reentry program offered in Arizona state-run prison units. This study evaluates the success of this program through semi-structured interviews with correctional officers and incarcerated men and women at two Arizona prison units. These results are contextualized within the history of rehabilitative program evaluation from the "nothing works" paradigm of the 1970s through contemporary Good Lives Model and desistance thinking.

Details

Contributors
Date Created
2024-05
Resource Type

Additional Information

English
Series
  • Academic Year 2023-2024
Extent
  • 48 pages
Open Access
Peer-reviewed