Histology, the microscopic examination of tissues, is a pivotal tool in research. By providing a detailed view of cellular structures and their organization, this technique allows researchers to advance knowledge in many different disciplines including women’s health and ovarian studies. Studying the female reproductive system is of utmost importance due to its multifaceted effects on aspects such as biological functions, cellular mechanisms, and complex behavioral patterns in women. Therefore, the aim of this honors thesis is to refine and adapt a set of protocols from the Mayer-Dyer Laboratory for ovarian and follicular processing so that they are optimal to use in the Bimonte-Nelson Laboratory of Memory and Aging in the Department of Psychology at Arizona State University. The subsequent aim for the thesis is to replicate and extend the new protocols to complete histology of the ovaries from an interdisciplinary study that evaluated the consequences of hysterectomy with and without estrogen replacement for brain and cognition. This dual-pronged approach will not only contribute to the ongoing discussion regarding the effects of hysterectomy, but also provides a valuable framework for future research endeavors in the field.