Description

Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an

Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated.

Reuse Permissions
  • application/pdf

    Download count: 0

    Details

    Contributors
    Date Created
    • 2016-12-15
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1063/1.4972069
    • Identifier Type
      International standard serial number
      Identifier Value
      2329-7778

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Kupitz, C., Olmos, J. L., Holl, M., Tremblay, L., Pande, K., Pandey, S., . . . Schmidt, M. (2017). Structural enzymology using X-ray free electron lasers. Structural Dynamics, 4(4), 044003. doi:10.1063/1.4972069

    Machine-readable links