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There are many proteomic applications that require large collections of purified protein, but parallel production of large numbers of different proteins remains a very challenging task. To help meet the

There are many proteomic applications that require large collections of purified protein, but parallel production of large numbers of different proteins remains a very challenging task. To help meet the needs of the scientific community, we have developed a human protein production pipeline. Using high-throughput (HT) methods, we transferred the genes of 31 full-length proteins into three expression vectors, and expressed the collection as N-terminal HaloTag fusion proteins in Escherichia coli and two commercial cell-free (CF) systems, wheat germ extract (WGE) and HeLa cell extract (HCE).

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Date Created
  • 2014-08-01
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  • Text
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    Identifier
    • Digital object identifier: 10.1002/pro.2484
    • Identifier Type
      International standard serial number
      Identifier Value
      0961-8368
    • Identifier Type
      International standard serial number
      Identifier Value
      1469-896X
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    Saul, Justin, Petritis, Brianne, Sau, Sujay, Rauf, Femina, Gaskin, Michael, Ober-Reynolds, Benjamin, Mineyev, Irina, Magee, Mitch, Chaput, John, Qiu, Ji, & LaBaer, Joshua (2014). Development of a full-length human protein production pipeline. PROTEIN SCIENCE, 23(8), 1123-1135. http://dx.doi.org/10.1002/pro.2484

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