Background: The USDA Fresh Fruit and Vegetable Program (FFVP) provides accessibility, variety, and nutrition education to increase exposure to fresh F/V to school children. The aim of this study examines Arizona FFVP’s nutrition education frequency and delivery in relation to the effects by the COVID-19 pandemic for SY 2019-2020, SY 2020-2021, and SY 2021 - 2022. Methods: A retrospective cross-sectional analysis of 57 Arizona school food managers (FSM) (and alike positions) (N=143; n=57; 18%(10) used Spanish; 82%(47) used English; FFVP site-level FSM = 81%(41); FFVP Non-FSM = 19% (11); 88% (50) rural; 12%(7) urban) during SY2019 – 2020, SY2020 – 2021, and SY2021 - 2022. Participants were surveyed for their perspective of FFVP service, nutrition education, and partnerships. The statistical analysis used quantitative and qualitative content variables presented as percentages. Results: Nutrition education were consistently delivered twice a week. FFVP service delivered most commonly 2 days/week (TF1 at 63% (26); TF2 at 59% (20),TF3 at 54% (19); TF 4 at 53% (19)). FFVP nutrition education was most frequently delivered 2days/week (TF1 at 55% (18); TF2 at 55% (18); TF3 at 54% (15); TF4 at 54% (20)). Teachers were most responsible for delivering nutrition education weekly in classrooms TF1 at 55%(21), TF2 at 44%(16), TF3 at 38% (15), TF4 at 45%. Most frequent method to select nutrition education was based on produce served that week (TF1 at 40% (23), TF2 at 36% (21), TF 3 at 39% (21),TF4 at 40% (24)) and day TF1 at 37% (21), TF2 at 36% (21), TF3 at 35% (19), TF4 42% (25). Conclusion: FSM and alike positions are important to FFVP services. Arizona FFVP schools were able provide consistent nutrition education even through a nationwide school closure caused by COVID-19 pandemic.

The glycation of plasma proteins leading to the production of advanced glycation end products (AGEs) and subsequent damage is a driving factor in the pathophysiology of diabetic complications. The overall research objective was to elucidate the mechanisms by which birds prevent protein glycation in the presence of naturally high plasma glucose concentrations. This was accomplished through the specific purpose of examining the impact of temperature and glucose concentration on the percent glycation of chicken serum albumin (CSA) in comparison to human serum albumin (HSA). Purified CSA and HSA solutions prepared at four different glucose concentrations (0 mM, 5.56 mM, 11.11 mM, and 22.22 mM) were incubated at three different temperatures (37.0°C, 39.8°C, and 41.4°C) on separate occasions for seven days with aliquots extracted on days 0, 3, and 7. Samples were analyzed by LC-ESI-MS for percent glycation of albumin. The statistically significant interaction between glucose concentration, temperature, albumin type, and time as determined by four-way repeated measures ANOVA (p = 0.032) indicated that all independent variables interacted to affect the mean percent glycation of albumin. As glucose concentration increased, the percent glycation of both HSA and CSA increased over time at all temperatures. In addition, HSA was glycated to a greater extent than CSA at the two higher glucose concentrations examined for all temperature conditions. Temperature differentially affected percent glycation of HSA and CSA wherein glycation increased with rising temperatures for HSA but not CSA. The results of this study suggest an inherent difference between the human and chicken albumin that contributes to the observed differences in glycation. Further research is needed to characterize this inherent difference in an effort to elucidate the mechanism by which birds protect plasma proteins from glycation. Future related work has the potential to lead to the development of novel therapies to prevent or reverse protein glycation prior to the formation of AGEs in humans, thus preventing the development and devastating effects of numerous diabetic complications.