Full metadata
Title
Optimizing Transfection, Expression, and Purification of the Human Transient Receptor Potential Melastatin 8 (hTRPM8) from HEK293 cells
Description
Transient Receptor Potential Melastatin 8 (TRPM8) is a non-selective cation channel notable as a primary cold sensor in humans. It is also involved in a variety of (patho)physiological events including pain sensation, chronic cough, diabetes, obesity, and cancer. TRPM8 is modulated by a variety of stimuli including pH, temperature, cooling agents, voltage, lipid, and other proteins. However, the molecular mechanism underlining its function has not yet clear raising the need for isolated proteins to be well-characterized. Over 20 years, E. coli has been a heterologous expression system of interest due to its low cost and high yield. However, the lack of post-translational modifications and chaperone may cause a misfolding or affect protein function. Mammalian expression system addresses these drawbacks and is a good candidate for the functional study of complex human protein. Here I describe my research in optimizing the transfection, expression, and purification of the human TRPM8 from adherent Human Embryonic Kidney (HEK293) cells which can be used for small-scale studies including, but not limited to, planar lipid bilayer electrophysiology.
Date Created
2017-12
Contributors
- Nguyen, Hoang Phuong My (Author)
- Van Horn, Wade (Thesis director)
- Wang, Xu (Committee member)
- Hilton, Jacob (Committee member)
- School of Life Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- Computing and Informatics Program (Contributor)
- Barrett, The Honors College (Contributor)
Topical Subject
Resource Type
Extent
36 pages
Language
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2017-2018
Handle
https://hdl.handle.net/2286/R.I.45649
Level of coding
minimal
Cataloging Standards
System Created
- 2017-10-30 02:50:58
System Modified
- 2021-08-11 04:09:57
- 2 years 8 months ago
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