Description

Viral protein U (Vpu) is a type-III integral membrane protein encoded by Human Immunodeficiency Virus-1 (HIV- 1). It is expressed in infected host cells and plays several roles in viral

Viral protein U (Vpu) is a type-III integral membrane protein encoded by Human Immunodeficiency Virus-1 (HIV- 1). It is expressed in infected host cells and plays several roles in viral progeny escape from infected cells, including down-regulation of CD4 receptors. But key structure/function questions remain regarding the mechanisms by which the Vpu protein contributes to HIV-1 pathogenesis. Here we describe expression of Vpu in bacteria, its purification and characterization.

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    Date Created
    • 2017-02-22
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.1371/journal.pone.0172529
    • Identifier Type
      International standard serial number
      Identifier Value
      1045-3830
    • Identifier Type
      International standard serial number
      Identifier Value
      1939-1560

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    Deb, A., Johnson, W. A., Kline, A. P., Scott, B. J., Meador, L. R., Srinivas, D., . . . Mor, T. S. (2017). Bacterial expression, correct membrane targeting and functional folding of the HIV-1 membrane protein Vpu using a periplasmic signal peptide. Plos One, 12(2). doi:10.1371/journal.pone.0172529

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