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Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application

Antibodies are essential for structural determinations and functional studies of membrane proteins, but antibody generation is limited by the availability of properly-folded and purified antigen. We describe the first application of genetic immunization to a structurally diverse set of membrane proteins to show that immunization of mice with DNA alone produced antibodies against 71% (n = 17) of the bacterial and viral targets. Antibody production correlated with prior reports of target immunogenicity in host organisms, underscoring the efficiency of this DNA-gold micronanoplex approach.

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    Date Created
    • 2016-02-24
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  • Text
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    Identifier
    • Digital object identifier: 10.1038/srep21925
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
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    Hansen, D. T., Robida, M. D., Craciunescu, F. M., Loskutov, A. V., Dörner, K., Rodenberry, J., . . . Sykes, K. F. (2016). Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization. Scientific Reports, 6(1). doi:10.1038/srep21925

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