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Polymerases that synthesize artificial genetic polymers hold great promise for advancing future applications in synthetic biology. However, engineering natural polymerases to replicate unnatural genetic polymers is a challenging problem. Here

Polymerases that synthesize artificial genetic polymers hold great promise for advancing future applications in synthetic biology. However, engineering natural polymerases to replicate unnatural genetic polymers is a challenging problem. Here we present droplet-based optical polymerase sorting (DrOPS) as a general strategy for expanding polymerase function that employs an optical sensor to monitor polymerase activity inside the microenvironment of a uniform synthetic compartment generated by microfluidics.

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    Date Created
    • 2016-04-05
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    Identifier
    • Digital object identifier: 10.1038/ncomms11235
    • Identifier Type
      International standard serial number
      Identifier Value
      2041-1723
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    Larsen, A. C., Dunn, M. R., Hatch, A., Sau, S. P., Youngbull, C., & Chaput, J. C. (2016). A general strategy for expanding polymerase function by droplet microfluidics. Nature Communications, 7, 11235. doi:10.1038/ncomms11235

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