Three-dimensional models of human intestinal epithelium mimic the differentiated form and function of parental tissues often not exhibited by two-dimensional monolayers and respond to Salmonella in key ways that reflect in vivo infections. To further enhance the physiological relevance of three-dimensional models to more closely approximate in vivo intestinal microenvironments encountered by Salmonella, we developed and validated a novel three-dimensional co-culture infection model of colonic epithelial cells and macrophages using the NASA Rotating Wall Vessel bioreactor.
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- Digital object identifier: 10.1038/s41526-017-0011-2
- Identifier TypeInternational standard serial numberIdentifier Value2373-8065
- The final version of this article, as published in Npj Microgravity, can be viewed online at: http://www.nature.com/articles/s41526-017-0011-2, opens in a new window
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Barrila, J., Yang, J., Crabbé, A., Sarker, S. F., Liu, Y., Ott, C. M., . . . Nickerson, C. A. (2017). Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. Npj Microgravity, 3(1). doi:10.1038/s41526-017-0011-2