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Since nitrogen (N) is often limiting in permafrost soils, we investigated the N[subscript 2]-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples

Since nitrogen (N) is often limiting in permafrost soils, we investigated the N[subscript 2]-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal soil. Samples from minimally, moderately and extensively thawed sites were taken to a depth of 79 cm to encompass zones above and below the depth of the water table. NifH reads were translated with frameshift correction and 112,476 sequences were clustered at 5% amino acid dissimilarity resulting in 1,631 OTUs.

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    Date Created
    • 2016-11-24
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.3389/fmicb.2016.01894
    • Identifier Type
      International standard serial number
      Identifier Value
      1664-1078

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    Penton, C. R., Yang, C., Wu, L., Wang, Q., Zhang, J., Liu, F., . . . Zhou, J. (2016). NifH-Harboring Bacterial Community Composition across an Alaskan Permafrost Thaw Gradient. Frontiers in Microbiology, 7. doi:10.3389/fmicb.2016.01894

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