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We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also

We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.

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    Date Created
    • 2016-09-20
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.3791/54463
    • Identifier Type
      International standard serial number
      Identifier Value
      1940-087X
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    Ishchenko, A., Cherezov, V., & Liu, W. (2016). Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. Journal of Visualized Experiments, (115). doi:10.3791/54463

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