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- Creators: ASU Marketing Hub
- Creators: Pellegrino, Laura
- Creators: Nelson, Garrett
Description
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
ContributorsOberthuer, Dominik (Author) / Knoska, Juraj (Author) / Wiedorn, Max O. (Author) / Beyerlein, Kenneth R. (Author) / Bushnell, David A. (Author) / Kovaleva, Elena G. (Author) / Heymann, Michael (Author) / Gumprecht, Lars (Author) / Kirian, Richard (Author) / Barty, Anton (Author) / Mariani, Valerio (Author) / Tolstikova, Aleksandra (Author) / Adriano, Luigi (Author) / Awel, Salah (Author) / Barthelmess, Miriam (Author) / Dorner, Katerina (Author) / Xavier, P. Lourdu (Author) / Yefanov, Oleksandr (Author) / James, Daniel (Author) / Nelson, Garrett (Author) / Wang, Dingjie (Author) / Calvey, George (Author) / Chen, Yujie (Author) / Schmidt, Andrea (Author) / Szczepek, Michael (Author) / Frielingsdorf, Stefan (Author) / Lenz, Oliver (Author) / Snell, Edward (Author) / Robinson, Philip J. (Author) / Sarler, Bozidar (Author) / Belsak, Grega (Author) / Macek, Marjan (Author) / Wilde, Fabian (Author) / Aquila, Andrew (Author) / Boutet, Sebastien (Author) / Liang, Mengning (Author) / Hunter, Mark S. (Author) / Scheerer, Patrick (Author) / Lipscomb, John D. (Author) / Weierstall, Uwe (Author) / Kornberg, Roger D. (Author) / Spence, John (Author) / Pollack, Lois (Author) / Chapman, Henry N. (Author) / Bajt, Sasa (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor)
Created2017-03-16
Description
X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LCLS), SLAC National Laboratory, exposing single inorganic core-shell nanoparticles to femtosecond hard-X-ray pulses. Each facetted nanoparticle consisted of a crystalline gold core and a differently shaped palladium shell. Scattered intensities were observed up to about 7 nm resolution. Analysis of the scattering patterns revealed the size distribution of the samples, which is consistent with that obtained from direct real-space imaging by electron microscopy. Scattering patterns resulting from single particles were selected and compiled into a dataset which can be valuable for algorithm developments in single particle scattering research.
ContributorsLi, Xuanxuan (Author) / Chiu, Chun-Ya (Author) / Wang, Hsiang-Ju (Author) / Kassemeyer, Stephan (Author) / Botha, Sabine (Author) / Shoeman, Robert L. (Author) / Lawrence, Robert (Author) / Kupitz, Christopher (Author) / Kirian, Richard (Author) / James, Daniel (Author) / Wang, Dingjie (Author) / Nelson, Garrett (Author) / Messerschmidt, Marc (Author) / Boutet, Sebastien (Author) / Williams, Garth J. (Author) / Hartman, Elisabeth (Author) / Jafarpour, Aliakbar (Author) / Foucar, Lutz M. (Author) / Barty, Anton (Author) / Chapman, Henry (Author) / Liang, Mengning (Author) / Menzel, Andreas (Author) / Wang, Fenglin (Author) / Basu, Shibom (Author) / Fromme, Raimund (Author) / Doak, R. Bruce (Author) / Fromme, Petra (Author) / Weierstall, Uwe (Author) / Huang, Michael H. (Author) / Spence, John (Author) / Schlichting, Ilme (Author) / Hogue, Brenda (Author) / Liu, Haiguang (Author) / ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / College of Liberal Arts and Sciences (Contributor) / School of Molecular Sciences (Contributor) / Department of Physics (Contributor) / School of Life Sciences (Contributor)
Created2017-04-11
Description
Single particle diffractive imaging data from Rice Dwarf Virus (RDV) were recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). RDV was chosen as it is a well-characterized model system, useful for proof-of-principle experiments, system optimization and algorithm development. RDV, an icosahedral virus of about 70 nm in diameter, was aerosolized and injected into the approximately 0.1 μm diameter focused hard X-ray beam at the CXI instrument of LCLS. Diffraction patterns from RDV with signal to 5.9 Ångström were recorded. The diffraction data are available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development, the contents of which are described here.
ContributorsMunke, Anna (Author) / Andreasson, Jakob (Author) / Aquila, Andrew (Author) / Awel, Salah (Author) / Ayyer, Kartik (Author) / Barty, Anton (Author) / Bean, Richard J. (Author) / Berntsen, Peter (Author) / Bielecki, Johan (Author) / Boutet, Sebastien (Author) / Bucher, Maximilian (Author) / Chapman, Henry N. (Author) / Daurer, Benedikt J. (Author) / DeMirci, Hasan (Author) / Elser, Veit (Author) / Fromme, Petra (Author) / Hajdu, Janos (Author) / Hantke, Max F. (Author) / Higashiura, Akifumi (Author) / Hogue, Brenda (Author) / Hosseinizadeh, Ahmad (Author) / Kim, Yoonhee (Author) / Kirian, Richard (Author) / Reddy, Hemanth K. N. (Author) / Lan, Ti-Yen (Author) / Larsson, Daniel S. D. (Author) / Liu, Haiguang (Author) / Loh, N. Duane (Author) / Maia, Filipe R. N. C. (Author) / Mancuso, Adrian P. (Author) / Muhlig, Kerstin (Author) / Nakagawa, Atsushi (Author) / Nam, Daewoong (Author) / Nelson, Garrett (Author) / Nettelblad, Carl (Author) / Okamoto, Kenta (Author) / Ourmazd, Abbas (Author) / Rose, Max (Author) / van der Schot, Gijs (Author) / Schwander, Peter (Author) / Seibert, M. Marvin (Author) / Sellberg, Jonas A. (Author) / Sierra, Raymond G. (Author) / Song, Changyong (Author) / Svenda, Martin (Author) / Timneanu, Nicusor (Author) / Vartanyants, Ivan A. (Author) / Westphal, Daniel (Author) / Wiedom, Max O. (Author) / Williams, Garth J. (Author) / Xavier, Paulraj Lourdu (Author) / Soon, Chun Hong (Author) / Zook, James (Author) / College of Liberal Arts and Sciences (Contributor, Contributor) / School of Molecular Sciences (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / School of Life Sciences (Contributor) / Infectious Diseases and Vaccinology (Contributor) / Department of Physics (Contributor)
Created2016-08-01
Description
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
ContributorsNogly, Przemyslaw (Author) / Panneels, Valerie (Author) / Nelson, Garrett (Author) / Gati, Cornelius (Author) / Kimura, Tetsunari (Author) / Milne, Christopher (Author) / Milathianaki, Despina (Author) / Kubo, Minoru (Author) / Wu, Wenting (Author) / Conrad, Chelsie (Author) / Coe, Jesse (Author) / Bean, Richard (Author) / Zhao, Yun (Author) / Bath, Petra (Author) / Dods, Robert (Author) / Harimoorthy, Rajiv (Author) / Beyerlein, Kenneth R. (Author) / Rheinberger, Jan (Author) / James, Daniel (Author) / Deponte, Daniel (Author) / Li, Chufeng (Author) / Sala, Leonardo (Author) / Williams, Garth J. (Author) / Hunter, Mark S. (Author) / Koglin, Jason E. (Author) / Berntsen, Peter (Author) / Nango, Eriko (Author) / Iwata, So (Author) / Chapman, Henry N. (Author) / Fromme, Petra (Author) / Frank, Matthias (Author) / Abela, Rafael (Author) / Boutet, Sebastien (Author) / Barty, Anton (Author) / White, Thomas A. (Author) / Weierstall, Uwe (Author) / Spence, John (Author) / Neutze, Richard (Author) / Schertler, Gebhard (Author) / Standfuss, Jorg (Author) / College of Liberal Arts and Sciences (Contributor) / Department of Physics (Contributor) / Department of Chemistry and Biochemistry (Contributor) / Biodesign Institute (Contributor) / Applied Structural Discovery (Contributor) / School of Molecular Sciences (Contributor)
Created2016-08-22
Description
When renowned cartographer and mountaineer Brad Washburn visited the Grand Canyon in 1969, he discovered that existing maps of the area were "inadequate" for either popular or scholarly use. Never one to be deterred, Washburn set about making one. This is the story of his 7-year-long effort, done in close collaboration with the National Geographic Society, Switzerland's Federal Office of Topography, and scores of supporting characters, to satisfy his desire to produce the best map of the canyon -- more precise, more detailed, more beautiful -- than anything that had come before.
ContributorsFry, Michael (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-02-28
Description
In 1985, Arizona Governor Bruce Babbitt and several others officially registered the Grand Canyon Trust as a non-profit organization dedicated to defending the natural integrity of the Grand Canyon. But the Trust realized early on that issues don’t stop at the boundaries of Grand Canyon National Park. So in 1987, we expanded the scope of our work to encompass the entire Colorado Plateau, of which the Grand Canyon stands as the centerpiece. GIS at the Trust helps tell the historic and current conservation story through advanced cartography, interactive web mapping, and spatial analysis. Using art and science, we design maps that illustrate physical characteristics, cultural values, proposals and conservation actions, and vulnerabilities across the Colorado Plateau. Our work reaches a broad audience including policy-makers, constituencies, government agencies, and our supporters. This presentation will highlight some of our most recent work in and around Grand Canyon, challenges we face as geographers, and how our maps have been used to further protect the Grand Canyon.
ContributorsSmith, Stephanie (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-03-01
Description
Grand Canyon is a dynamic natural landscape that encodes nearly two billion years of geological history, and which is also situated within a cultural landscape that encodes the names, experiences, and lives of people from ancestral Native Americans to American explorers and settlers to modern visitors from across the nation and around the world. Place-based ways of teaching integrate the natural and the cultural attributes of a place or region such as Grand Canyon to facilitate learning. For the last century, Grand Canyon National Park has offered interpretive programs and resources to visitors that hew to this place-based philosophy, enabling millions of Park visitors to make intellectual and emotional connections to the landscape and its natural and cultural history. Geological and educational research have contributed to the interpretive mission of the Park with new research-based resources such as the Trail of Time Exhibition. Even more recently, advances in visualization and instructional technology have brought the pedagogical power of Grand Canyon to the online realm through immersive, interactive virtual field trips (iVFTs), which have the potential to enable many millions more to explore and learn from the natural and cultural landscapes of Grand Canyon, including its most physically inaccessible places. Current research is directed toward rendering iVFTs ever more authentic and place-based, while also enhancing the accessibility and effectiveness of in-person field experiences for visitors and students at Grand Canyon.
ContributorsSemken, Steven (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-03-01
Description
Over 700 known deaths have occurred in Grand Canyon from the first river exploration by John Wesley Powell in 1869 to present day. Causes range from suicides to accidental drownings, heatstroke, snake bite, flash floods, aircraft collisions, crashes and even murder. Based on the book ‘Over the Edge: Death in Grand Canyon’ by Michael Ghiglieri and Thomas Myers, this map illustrates the geography of deathly incidents. It uses a pan-sharpening technique to create a crisp, vibrant combination of layer tints and hillshades. The colours are defined to allow the map to be viewed in normal two dimensional viewing but in 3D when viewed using chromadepth glasses. The map provides a dramatic, visually engaging illustration of a unique dataset and maintain the first geocoded display of the complete record of deaths in Grand Canyon. In so doing, it illustrates the development and application of novel cartographic approaches. Vignettes describing the incidents bring the quantity of death into perspective through the telling of short individual stories, some fantastic, some tragic. The presentation will discuss the map’s creation in 2012, a recent update, and also the response to its publication. There were some very real issues faced in portraying an often sensitive subject matter, and some of the failures in this respect and the lessons learned will be explored.
ContributorsField, Kenneth (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-03-01
DescriptionIn this presentation, Spindler will describe the work of designing and building the 100 Years of Grand collaborative digital archive and the associated challenges of item selection, description, copyright, and project coordination. He will also demonstrate the digital archive and present examples of key items of Grand Canyon history.
ContributorsSpindler, Rob (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-03-01
Description
The passage of the Grand Canyon Protection Act (1992) and the completion of the Glen Canyon Dam Environmental Impact Statement (1996) ushered in a new era of environmental monitoring and research of the Colorado River corridor in Grand Canyon. Technological advancements in surveying and mapping systems over this period have made it possible to map larger areas with an increasing level of precision and accuracy. All of these mapping efforts rely on an accurate geodetic control network along the rim and inner canyon corridor. Examples of mapping efforts include aerial photographic, topographic, and bathymetric missions. Aerial overflights of the entire canyon corridor have been conducted in 2002, 2009, and 2013 and the high-resolution orthophographs and photogrammetrically-derived topography form the base data set for a number of investigations. From 2009 to 2017, over 160 miles of channel have been mapped using multibeam bathymetry. The bathymetric maps reveal the form of the Channel bed and allow researchers to asses flow operations from Glen Canyon dam on the sediment resources within the Colorado River ecosystem.
ContributorsKaplinski, Matt (Author, Speaker) / ASU Marketing Hub (Videographer)
Created2019-03-01