ASU Electronic Theses and Dissertations
This collection includes most of the ASU Theses and Dissertations from 2011 to present. ASU Theses and Dissertations are available in downloadable PDF format; however, a small percentage of items are under embargo. Information about the dissertations/theses includes degree information, committee members, an abstract, supporting data or media.
In addition to the electronic theses found in the ASU Digital Repository, ASU Theses and Dissertations can be found in the ASU Library Catalog.
Dissertations and Theses granted by Arizona State University are archived and made available through a joint effort of the ASU Graduate College and the ASU Libraries. For more information or questions about this collection contact or visit the Digital Repository ETD Library Guide or contact the ASU Graduate College at gradformat@asu.edu.
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ano-oscillators, are developed to measure the binding-induced charge change instead of the mass change, which enables quantification of the binding kinetics for both large and small molecules.
In particular, the nano-oscillator approach provides a unique capability to image individual nanoparticles and measure the size and charge of each nanoparticle simultaneously. This approach is applied to measure one of the smallest biological particles - single protein molecules. By tracking the oscillation of each protein molecule, the size, charge, and mobility are measured in real-time with high precision. This capability also allows to monitor the conformation and charge changes of single protein molecules upon ligand binding. Measuring the size and charge of single proteins opens a new revenue to protein analysis and disease biomarker detection at the single molecule level.
The virion
ano-oscillators and the single protein approach employ a scheme where a particle is tethered to the surface with a polymer molecule. The dynamics of the particle is governed by two important forces: One is entropic force arising from the conformational change of the molecular tether, and the other is solvent damping on the particle and the molecule. The dynamics is studied by varying the type of the tether molecule, size of the particle, and viscosity of the solvent. The findings provide insights into single molecule studies using not only tethered particles, but also other approaches, including force spectroscopy using atomic force microscopy and nanopores.