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Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The Performance Based Studies Research Group (PBSRG) has developed industry-tested leadership and management techniques that have been proven to increase organizational performance. The Leadership Society of Arizona (LSA) has worked closely with PBSRG to develop an educational framework that introduces these leadership concepts to college students. LSA is now endeavoring to make this curriculum more accessible for K-12 students and educators. As part of a thesis creative project, the author has developed a strategy to connect with and enable local high schools, teachers, and students to engage with the professional industry and higher education. This strategy will allow LSA to connect with up to 150 high school students over the summer of 2016. By making this education easily accessible, the author has accomplished a milestone in the larger effort encompassed by LSA. The course chosen to present to high school students is an abridged variation of the Barrett Honors College course "Deductive Logic: Leadership and Management Techniques". The class framework is designed to instantiate a self-sustaining program for future summer school courses. The summer school course will allow high school students to learn, understand, and apply college level concepts into their education, work, and personal lives. The development of the framework for the program encompasses networking/partnering efforts, marketing package creation, and the delivery of the summer school course over the months of June and July in 2016.
ContributorsDunn, Melissa Anne (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Industrial, Systems (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
With the help of some Information Measurement Theory (IMT), Kashiwagi Solutions Model (KSM), and deductive logic background, supply chain managers can start utilizing a new way to effectively and efficiently negotiate contracts. Developed by Dr. Dean Kashiwagi, the Best Value Approach has been 98% successful with over 1,800 projects for the past 20 years. The process gives vendors/suppliers the power to use their expertise. In return for not having to follow the rules set by the client/buyer, the vendor must show documentation and plans of risk management, value added processes, and metrics.
ContributorsPhan, Alice (Co-author) / Holtzman, Krista (Co-author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / School of International Letters and Cultures (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Since 1994, the Performance Based Studies Research Group at Arizona State University has utilized an approach to industry called Best Value (BV). Since its origin, this approach has been used in 1860 tests creating $6.4 billion dollars of projects and services delivered, at a customer satisfaction rating of 95%. Best Value (BV) is rooted in simplicity, and seeks to help organizations hire experts, plan ahead, minimize risk, optimize resources, and optimize resources. This is accomplished largely through the use of a tool the PBSRG calls the Kashiwagi Solution Model (KSM). Kashiwagi Solution Models can be used across every industry from construction to Wall Street to help achieve sustainable success in what is perhaps the most efficient and effective manner available today. Using Best Value (BV) and the Kashiwagi Solution Model (KSM), the author identified groups on Wall Street and throughout the world who deal in a unique entity called "Over-The-Counter (OTC) Derivatives". More specifically, this paper focuses on the current status and ramifications of derivative contracts that two parties enter with the sole intention of speculating. KSMs are used in Information Measurement Theory, which seeks to take seemingly complex subjects and simplify them into terms that everyone can understand. This document uses Information Measurement Theory to explain what OTC derivatives are in the simplest possible way, so that little prior knowledge of finance is required to understand the material. Through research and observation, KSMs can be used to identify the characteristics of groups who deal in OTC derivatives, which contributed to the financial crisis in 2008 and have grown in size and complexity. This document uses dominant information in order to see the potential problems within the OTC derivatives market from 30,000 feet, and offer solutions to those problems. Keywords: simplicity, best value approach, identify characteristics, dominant information
ContributorsBills, Andrew Marius (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Rivera, Alfredo (Committee member) / Department of Finance (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05