Matching Items (145)
Description
One of the fundamental questions in molecular biology is how genes and the control of their expression give rise to so many diverse phenotypes in nature. The mRNA molecule plays a key role in this process as it directs the spatial and temporal expression of genetic information contained in the DNA molecule to precisely instruct biological processes in living organisms. The region located between the STOP codon and the poly(A)-tail of the mature mRNA, known as the 3′Untranslated Region (3′UTR), is a key modulator of these activities. It contains numerous sequence elements that are targeted by trans-acting factors that dose gene expression, including the repressive small non-coding RNAs, called microRNAs.
Recent transcriptome data from yeast, worm, plants, and humans has shown that alternative polyadenylation (APA), a mechanism that enables expression of multiple 3′UTR isoforms for the same gene, is widespread in eukaryotic organisms. It is still poorly understood why metazoans require multiple 3′UTRs for the same gene, but accumulating evidence suggests that APA is largely regulated at a tissue-specific level. APA may direct combinatorial variation between cis-elements and microRNAs, perhaps to regulate gene expression in a tissue-specific manner. Apart from a few single gene anecdotes, this idea has not been systematically explored.
This dissertation research employs a systems biology approach to study the somatic tissue dynamics of APA and its impact on microRNA targeting networks in the small nematode C. elegans. In the first aim, tools were developed and applied to isolate and sequence mRNA from worm intestine and muscle tissues, which revealed pervasive tissue-specific APA correlated with microRNA regulation. The second aim provides genetic evidence that two worm genes use APA to escape repression by microRNAs in the body muscle. Finally, in aim three, mRNA from five additional somatic worm tissues was sequenced and their 3′ends mapped, allowing for an integrative study of APA and microRNA targeting dynamics in worms. Together, this work provides evidence that APA is a pervasive mechanism operating in somatic tissues of C. elegans with the potential to significantly rearrange their microRNA regulatory networks and precisely dose their gene expression.
Recent transcriptome data from yeast, worm, plants, and humans has shown that alternative polyadenylation (APA), a mechanism that enables expression of multiple 3′UTR isoforms for the same gene, is widespread in eukaryotic organisms. It is still poorly understood why metazoans require multiple 3′UTRs for the same gene, but accumulating evidence suggests that APA is largely regulated at a tissue-specific level. APA may direct combinatorial variation between cis-elements and microRNAs, perhaps to regulate gene expression in a tissue-specific manner. Apart from a few single gene anecdotes, this idea has not been systematically explored.
This dissertation research employs a systems biology approach to study the somatic tissue dynamics of APA and its impact on microRNA targeting networks in the small nematode C. elegans. In the first aim, tools were developed and applied to isolate and sequence mRNA from worm intestine and muscle tissues, which revealed pervasive tissue-specific APA correlated with microRNA regulation. The second aim provides genetic evidence that two worm genes use APA to escape repression by microRNAs in the body muscle. Finally, in aim three, mRNA from five additional somatic worm tissues was sequenced and their 3′ends mapped, allowing for an integrative study of APA and microRNA targeting dynamics in worms. Together, this work provides evidence that APA is a pervasive mechanism operating in somatic tissues of C. elegans with the potential to significantly rearrange their microRNA regulatory networks and precisely dose their gene expression.
ContributorsBlazie, Stephen M (Author) / Mangone, Marco (Thesis advisor) / LaBaer, Josh (Committee member) / Lake, Doug (Committee member) / Newfeld, Stuart (Committee member) / Arizona State University (Publisher)
Created2016
Description
The Performance Based Studies Research Group (PBSRG) has developed industry-tested leadership and management techniques that have been proven to increase organizational performance. The Leadership Society of Arizona (LSA) has worked closely with PBSRG to develop an educational framework that introduces these leadership concepts to college students. LSA is now endeavoring to make this curriculum more accessible for K-12 students and educators. As part of a thesis creative project, the author has developed a strategy to connect with and enable local high schools, teachers, and students to engage with the professional industry and higher education. This strategy will allow LSA to connect with up to 150 high school students over the summer of 2016. By making this education easily accessible, the author has accomplished a milestone in the larger effort encompassed by LSA. The course chosen to present to high school students is an abridged variation of the Barrett Honors College course "Deductive Logic: Leadership and Management Techniques". The class framework is designed to instantiate a self-sustaining program for future summer school courses. The summer school course will allow high school students to learn, understand, and apply college level concepts into their education, work, and personal lives. The development of the framework for the program encompasses networking/partnering efforts, marketing package creation, and the delivery of the summer school course over the months of June and July in 2016.
ContributorsDunn, Melissa Anne (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Industrial, Systems (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Socks + Boxers began as a simple idea on a trip through Victoria's Secret as a solution to the lack of choice in quality and affordable undergarments for men. Currently, there is no central hub for men's socks and underwear. Customers shopping for men's undergarments have few choices currently: purchase an inexpensive, average quality, predetermined pack of Hanes, Fruit of the Loom, or other common undergarment brand from a store like Walmart or Target; shop for individual pieces of expensive designer underwear at a high-end department store such as Nordstrom; or, finally, purchase slightly above average quality, but fairly expensive, brand name undergarments at physical stores such as American Eagle, Urban Outfitters, or Abercrombie & Fitch, or online stores such as MeUndies. Socks + Boxers seeks to combine the accessibility and reliability of common undergarment brands, the quality and luxury-feel of high-end undergarments, and the concept of choice provided by stores that sell men's undergarment lines into a single business. We also plan to tap into the booming subscription services industry and create a way for customers to easily update and replenish their undergarment wardrobe on a regular basis with exactly what they want. In order to start out on the right foot and begin developing this business plan from the ground up, we began researching and developing a Business Model Canvas, a tool that breaks out necessary pieces of a successful business plan into easy to understand blocks. We took a critical look at the problem at hand, its potential solutions, the value the solutions provide, how we plan to start, grow, and nourish our customer base, and much more. The different pieces of this business model puzzle all come together in the following pages.
ContributorsBernat, Johnathon (Co-author) / Braaten, Joshua (Co-author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Department of Finance (Contributor) / Department of Information Systems (Contributor) / School of Accountancy (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
With the help of some Information Measurement Theory (IMT), Kashiwagi Solutions Model (KSM), and deductive logic background, supply chain managers can start utilizing a new way to effectively and efficiently negotiate contracts. Developed by Dr. Dean Kashiwagi, the Best Value Approach has been 98% successful with over 1,800 projects for the past 20 years. The process gives vendors/suppliers the power to use their expertise. In return for not having to follow the rules set by the client/buyer, the vendor must show documentation and plans of risk management, value added processes, and metrics.
ContributorsPhan, Alice (Co-author) / Holtzman, Krista (Co-author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / School of International Letters and Cultures (Contributor) / Department of Supply Chain Management (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Since 1994, the Performance Based Studies Research Group at Arizona State University has utilized an approach to industry called Best Value (BV). Since its origin, this approach has been used in 1860 tests creating $6.4 billion dollars of projects and services delivered, at a customer satisfaction rating of 95%. Best Value (BV) is rooted in simplicity, and seeks to help organizations hire experts, plan ahead, minimize risk, optimize resources, and optimize resources. This is accomplished largely through the use of a tool the PBSRG calls the Kashiwagi Solution Model (KSM). Kashiwagi Solution Models can be used across every industry from construction to Wall Street to help achieve sustainable success in what is perhaps the most efficient and effective manner available today. Using Best Value (BV) and the Kashiwagi Solution Model (KSM), the author identified groups on Wall Street and throughout the world who deal in a unique entity called "Over-The-Counter (OTC) Derivatives". More specifically, this paper focuses on the current status and ramifications of derivative contracts that two parties enter with the sole intention of speculating. KSMs are used in Information Measurement Theory, which seeks to take seemingly complex subjects and simplify them into terms that everyone can understand. This document uses Information Measurement Theory to explain what OTC derivatives are in the simplest possible way, so that little prior knowledge of finance is required to understand the material. Through research and observation, KSMs can be used to identify the characteristics of groups who deal in OTC derivatives, which contributed to the financial crisis in 2008 and have grown in size and complexity. This document uses dominant information in order to see the potential problems within the OTC derivatives market from 30,000 feet, and offer solutions to those problems. Keywords: simplicity, best value approach, identify characteristics, dominant information
ContributorsBills, Andrew Marius (Author) / Kashiwagi, Dean (Thesis director) / Kashiwagi, Jacob (Committee member) / Rivera, Alfredo (Committee member) / Department of Finance (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05