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Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension

Strategies are needed to improve repopulation of decellularized lung scaffolds with stromal and functional epithelial cells. We demonstrate that decellularized mouse lungs recellularized in a dynamic low fluid shear suspension bioreactor, termed the rotating wall vessel (RWV), contained more cells with decreased apoptosis, increased proliferation and enhanced levels of total RNA compared to static recellularization conditions. These results were observed with two relevant mouse cell types: bone marrow-derived mesenchymal stromal (stem) cells (MSCs) and alveolar type II cells (C10).

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    Date Created
    • 2015-05-11
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.1371/journal.pone.0126846
    • Identifier Type
      International standard serial number
      Identifier Value
      1045-3830
    • Identifier Type
      International standard serial number
      Identifier Value
      1939-1560

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    Crabbé, A., Liu, Y., Sarker, S. F., Bonenfant, N. R., Barrila, J., Borg, Z. D., . . . Nickerson, C. A. (2015). Recellularization of Decellularized Lung Scaffolds Is Enhanced by Dynamic Suspension Culture. Plos One, 10(5). doi:10.1371/journal.pone.0126846

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