Cervical cancer is the most common malignancy among women particularly in developing countries, with human papillomavirus (HPV) 16 causing 50% of invasive cervical cancers. A plant-based HPV vaccine is an alternative to the currently available virus-like particle (VLP) vaccines, and would be much less expensive. We optimized methods to express HPV16 L1 protein and purify VLPs from tobacco (Nicotiana benthamiana) leaves transfected with the magnICON deconstructed viral vector expression system.
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- Zahin, Maryam (Author)
- Joh, Joongho (Author)
- Khanal, Sujita (Author)
- Husk, Adam (Author)
- Mason, Hugh (Contributor)
- Warzecha, Heribert (Author)
- Ghim, Shin-je (Author)
- Miller, Donald M. (Author)
- Matoba, Nobuyuki (Author)
- Bennett Jenson, Alfred (Author)
- Immunotherapy, Vaccines and Virotherapy (Contributor)
- Biodesign Institute (Contributor)
- 2016-08-12
- Digital object identifier: 10.1371/journal.pone.0160995
- Identifier TypeInternational standard serial numberIdentifier Value1045-3830
- Identifier TypeInternational standard serial numberIdentifier Value1939-1560
- The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0160995, opens in a new window
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Zahin, M., Joh, J., Khanal, S., Husk, A., Mason, H., Warzecha, H., . . . Jenson, A. B. (2016). Scalable Production of HPV16 L1 Protein and VLPs from Tobacco Leaves. Plos One, 11(8). doi:10.1371/journal.pone.0160995