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We previously reported a recombinant protein production system based on a geminivirus replicon that yields high levels of vaccine antigens and monoclonal antibodies in plants. The bean yellow dwarf virus

We previously reported a recombinant protein production system based on a geminivirus replicon that yields high levels of vaccine antigens and monoclonal antibodies in plants. The bean yellow dwarf virus (BeYDV) replicon generates massive amounts of DNA copies, which engage the plant transcription machinery. However, we noticed a disparity between transcript level and protein production, suggesting that mRNAs could be more efficiently utilized. In this study, we systematically evaluated genetic elements from human, viral, and plant sources for their potential to improve the BeYDV system.

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    Date Created
    • 2016-02-24
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.3389/fpls.2016.00200
    • Identifier Type
      International standard serial number
      Identifier Value
      1664-462X

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    Diamos, A. G., Rosenthal, S. H., & Mason, H. S. (2016). 5′ and 3′ Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves. Frontiers in Plant Science, 7. doi:10.3389/fpls.2016.00200

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