Description

Mutations in ribosomal RNA (rRNA) have traditionally been detected by the primer extension assay, which is a tedious and multistage procedure. Here, we describe a simple and straightforward fluorescence assay

Mutations in ribosomal RNA (rRNA) have traditionally been detected by the primer extension assay, which is a tedious and multistage procedure. Here, we describe a simple and straightforward fluorescence assay based on binary deoxyribozyme (BiDz) sensors. The assay uses two short DNA oligonucleotides that hybridize specifically to adjacent fragments of rRNA, one of which contains a mutation site. This hybridization results in the formation of a deoxyribozyme catalytic core that produces the fluorescent signal and amplifies it due to multiple rounds of catalytic action.

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    Date Created
    • 2015-08-19
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.1261/rna.052613.115
    • Identifier Type
      International standard serial number
      Identifier Value
      1355-8382
    • Identifier Type
      International standard serial number
      Identifier Value
      1469-9001
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    Gerasimova, Y. V., Yakovchuk, P., Dedkova, L. M., Hecht, S. M., & Kolpashchikov, D. M. (2015). Expedited quantification of mutant ribosomal RNA by binary deoxyribozyme (BiDz) sensors. Rna, 21(10), 1834-1843. doi:10.1261/rna.052613.115

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