Description

Recent epigenetic association studies have identified a new gene, ANK1, in the pathogenesis of Alzheimer’s disease (AD). Although strong associations were observed, brain homogenates were used to generate the data,

Recent epigenetic association studies have identified a new gene, ANK1, in the pathogenesis of Alzheimer’s disease (AD). Although strong associations were observed, brain homogenates were used to generate the data, introducing complications because of the range of cell types analyzed. In order to address the issue of cellular heterogeneity in homogenate samples we isolated microglial, astrocytes and neurons by laser capture microdissection from CA1 of hippocampus in the same individuals with a clinical and pathological diagnosis of AD and matched control cases.

application/pdf

Download count: 0

Details

Contributors
Date Created
  • 2017-07-12
Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1371/journal.pone.0177814
    • Identifier Type
      International standard serial number
      Identifier Value
      1045-3830
    • Identifier Type
      International standard serial number
      Identifier Value
      1939-1560

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Mastroeni, D., Sekar, S., Nolz, J., Delvaux, E., Lunnon, K., Mill, J., . . . Coleman, P. D. (2017). ANK1 is up-regulated in laser captured microglia in Alzheimer’s brain; the importance of addressing cellular heterogeneity. Plos One, 12(7). doi:10.1371/journal.pone.0177814

    Machine-readable links