Description

The relationship between sequence and binding properties of an aptamer for immunoglobulin E (IgE) was investigated using custom DNA microarrays. Single, double and some triple mutations of the aptamer sequence

The relationship between sequence and binding properties of an aptamer for immunoglobulin E (IgE) was investigated using custom DNA microarrays. Single, double and some triple mutations of the aptamer sequence were created to evaluate the importance of specific base composition on aptamer binding. The majority of the positions in the aptamer sequence were found to be immutable, with changes at these positions resulting in more than a 100-fold decrease in binding affinity.

Reuse Permissions
  • application/pdf

    Download count: 0

    Details

    Contributors
    Date Created
    • 2007-12-01
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1093/nar/gkm922
    • Identifier Type
      International standard serial number
      Identifier Value
      1362-4962
    • Identifier Type
      International standard serial number
      Identifier Value
      0305-1048
    Note

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Katilius, E., Flores, C., & Woodbury, N. W. (2007). Exploring the sequence space of a DNA aptamer using microarrays. Nucleic Acids Research, 35(22), 7626-7635. doi:10.1093/nar/gkm922

    Machine-readable links