Description

Butyrylcholinesterase (BChE) is an enzyme with broad substrate and ligand specificities and may function as a generalized bioscavenger by binding and/or hydrolyzing various xenobiotic agents and toxicants, many of which

Butyrylcholinesterase (BChE) is an enzyme with broad substrate and ligand specificities and may function as a generalized bioscavenger by binding and/or hydrolyzing various xenobiotic agents and toxicants, many of which target the central and peripheral nervous systems. Variants of BChE were rationally designed to increase the enzyme’s ability to hydrolyze the psychoactive enantiomer of cocaine. These variants were cloned, and then expressed using the magnICON transient expression system in plants and their enzymatic properties were investigated.

Reuse Permissions
  • application/pdf

    Download count: 0

    Details

    Contributors
    Date Created
    • 2017-09-05
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1038/s41598-017-10571-z
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
    Note

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Larrimore, K. E., Kazan, I. C., Kannan, L., Kendle, R. P., Jamal, T., Barcus, M., . . . Mor, T. S. (2017). Plant-expressed cocaine hydrolase variants of butyrylcholinesterase exhibit altered allosteric effects of cholinesterase activity and increased inhibitor sensitivity. Scientific Reports, 7. doi:10.1038/s41598-017-10571-z

    Machine-readable links