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- Creators: LaBaer, Joshua
- Creators: Arizona State University. Libraries
- Creators: Aktipis, C. Athena
Using improv comedy skills to entertain students and keep their attention.
Survey instrument used in Survey of Academic Library Streaming Video Revisited, 2015
Explains the urgent need for libraries to engage in preservation of irreplaceable content on VHS and other obsolete video formats in their collections, and presents a database of titles for which due diligence as required by Section 108 of US Copyright has already been completed.
In spring 2013, the presenters developed a survey on academic library streaming video and distributed it broadly through various discussion and mailing lists.
This is the first large-scale and most comprehensive effort to date to collect data on streaming video funding, licensing, acquisition, and hosting in academic libraries. Its results will provide benchmark data for future explorations of this rapidly expanding approach to video in academic libraries.
Streaming video is becoming a common occurrence on many campuses today. Its fast growth is due in part to the steady growth of online classes and programs. Technology has also played a role in this growth as alternatives for ingesting and accessing content have expanded. Multiple options are now available including in-house approaches, cloud storage, and third party vendors.
This survey collected data on how academic institutions address the day-to-day operations related to streaming video as well as perceived directions for future action.
Survey questions addressed selection and acquisition of video in both hard copy and streaming formats, funding for acquisitions, current and planned hosting interfaces, cataloging and access, and current practice and policy on digitization of hard copy titles for streaming. This session reviews the instrument used, and provides a preliminary look at some of the key data collected.
Streaming video has been an option for academic libraries for nearly a decade. What is the state of streaming video in academic libraries today? How are these libraries acquiring streaming videos? Who makes acquisition decisions? How much staff time does supporting streaming video require?
There are many proteomic applications that require large collections of purified protein, but parallel production of large numbers of different proteins remains a very challenging task. To help meet the needs of the scientific community, we have developed a human protein production pipeline. Using high-throughput (HT) methods, we transferred the genes of 31 full-length proteins into three expression vectors, and expressed the collection as N-terminal HaloTag fusion proteins in Escherichia coli and two commercial cell-free (CF) systems, wheat germ extract (WGE) and HeLa cell extract (HCE). Expression was assessed by labeling the fusion proteins specifically and covalently with a fluorescent HaloTag ligand and detecting its fluorescence on a LabChip[superscript ®] GX microfluidic capillary gel electrophoresis instrument. This automated, HT assay provided both qualitative and quantitative assessment of recombinant protein. E. coli was only capable of expressing 20% of the test collection in the supernatant fraction with ≥20 μg yields, whereas CF systems had ≥83% success rates. We purified expressed proteins using an automated HaloTag purification method. We purified 20, 33, and 42% of the test collection from E. coli, WGE, and HCE, respectively, with yields ≥1 μg and ≥90% purity. Based on these observations, we have developed a triage strategy for producing full-length human proteins in these three expression systems.