ASU Electronic Theses and Dissertations
This collection includes most of the ASU Theses and Dissertations from 2011 to present. ASU Theses and Dissertations are available in downloadable PDF format; however, a small percentage of items are under embargo. Information about the dissertations/theses includes degree information, committee members, an abstract, supporting data or media.
In addition to the electronic theses found in the ASU Digital Repository, ASU Theses and Dissertations can be found in the ASU Library Catalog.
Dissertations and Theses granted by Arizona State University are archived and made available through a joint effort of the ASU Graduate College and the ASU Libraries. For more information or questions about this collection contact or visit the Digital Repository ETD Library Guide or contact the ASU Graduate College at gradformat@asu.edu.
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Description
Mast cells, components of the immune system, promote allergic symptoms such as itching, sneezing, and increased intestinal motility. Although mast cells have a detrimental role in allergies, they might have unrecognized physiological functions. Indeed, mast cells have been reported to protect against lethal envenomation. I hypothesized that mast cells have a protective role in the defense against toxins. Because toxin-induced diarrheal diseases are one of the top five causes of mortality in children worldwide (induced by cholera toxin, for example), I tested the role of mast cells in sensing relevant dietary toxins. My goals were to a) establish an in vitro model of mast cell activation using foodborne toxins and b) determine the mast cell transcriptional programs induced by these toxins. To establish the in vitro model, I generated mast cells from murine bone marrow precursors and cultured them in mast cell-specific media for 5 weeks. Mature mast cells were then stimulated with toxins from phylogenetically distinct origins. I found that, surprisingly, no toxin was able to induce significant cell death, even after 24h of culturing, suggesting that mast cells are resistant to the toxic effects of these compounds. To assess mast cell activation, I quantified the levels of TNF-α 6h after toxin exposure. None of the toxins were able to induce TNF-α production by mast cells, suggesting that toxins might not induce inflammation in mast cells. However, I found that mast cells induced expression of activation-related transcripts like Il1b, Tpsab1, Alox5, Egr1, Tnfa and Hdc in response to cholera toxin, when compared with controls. Mast cells stimulated with retrorsine induced the expression of Tph1, Alox5, Il1b and Hdc. Deoxynivalenol induced Ltc4, Il6, Tpsab1, Tnfa, Hdc, and Alox5 while okadaic acid induced Il6, Tnfa, Tph1, Alox5, Egr1, Il1b and Hdc expression. Aconitine only induced Il6, Hdc, and Tpsab1. Lastly, Ochratoxin A induced expression of Il1b, Il6, Tpsab1, Egr1 and Hdc. Altogether, these results suggest that mast cells directly sense and respond to food toxins, which was unknown. How exactly mast cells contribute to toxin defenses will be crucial to investigate as they impact both toxin-induced and inflammatory diseases.
ContributorsGalarza, Mayka (Author) / Borges Florsheim, Esther (Thesis advisor) / Lucas, Alexandra (Committee member) / Mana, Miyeko (Committee member) / Arizona State University (Publisher)
Created2023
Description
This study tested hypotheses derived from general strain theory, including whether the disposition for crime (e.g., trait anger and low self-control) and prosocial
coping moderate the link between state-based negative emotions and maladaptive coping.
A 2x2 factorial vignette was embedded in a survey instrument and administered to a
university-based sample (N = 503). The results from the regression models support
different parts of general strain theory, such as the direct effect of strain on negative
emotions, the effect of negative emotions on aggressive coping, and the mediating effect
of negative emotions. Finally, the effect of some negative emotions on aggressive coping
were moderated by trait anger, low self-control, and prosocial coping. The findings of
this study support the argument that dispositions to maladaptive coping amplify the
effects of negative emotion and underscore the importance of establishing prosocial
coping resources.
Keywords: emotions, general strain theory, low self-control, maladaptive coping
ContributorsWood, Margaret Tynan (Author) / Reisig, Michael D. (Thesis advisor) / Young, Jacob T.N. (Committee member) / Telep, Cody W. (Committee member) / Arizona State University (Publisher)
Created2023
Description
Evolutionary theory provides a rich framework for understanding cancer dynamics across scales of biological organization. The field of cancer evolution has largely been divided into two domains, comparative oncology - the study of cancer across the tree of life, and tumor evolution. This work provides a theoretical framework to unify these subfields with the intent that an understanding of the evolutionary dynamics driving cancer risk at one scale can inform the understanding of the dynamics on another scale. The evolution of multicellular life and the unique vulnerabilities in the cellular mechanisms that underpin it explain the ubiquity of cancer prevalence across the tree of life. The breakdown in cellular cooperation and communication that were required for multicellular life define the hallmarks of cancer. As divergent life histories drove speciation events, it similarly drove divergences in fundamental cancer risk across species. An understanding of the impact that species’ life history theory has on the underlying network of multicellular cooperation and somatic evolution allows for robust predictions on cross-species cancer risk. A large-scale veterinary cancer database is utilized to validate many of the predictions on cancer risk made from life history evolution. Changing scales to the cellular level, it lays predictions on the fate of somatic mutations and the fitness benefits they confer to neoplastic cells compared to their healthy counterparts. The cancer hallmarks, far more than just a way to unify the many seemingly unique pathologies defined as cancer, is a powerful toolset to understand how specific mutations may change the fitness of somatic cells throughout carcinogenesis and tumor progression. Alongside highlighting the significant advances in evolutionary approaches to cancer across scales, this work provides a lucid confirmation that an understanding of both scales provides the most complete portrait of evolutionary cancer dynamics.
ContributorsCompton, Zachary Taylor (Author) / Maley, Carlo C. (Thesis advisor) / Aktipis, Athena (Committee member) / Buetow, Kenneth (Committee member) / Nedelcu, Aurora (Committee member) / Compton, Carolyn (Committee member) / Arizona State University (Publisher)
Created2023
Description
Blalock’s (1967) minority threat perspective is one of the most empirically investigated theories of crime control in criminological literature. A large body of research has tested this perspective and established a link between minority context and increased criminal justice controls. The perceived threat mechanisms hypothesized to facilitate this link, however, have received relatively scant attention. In addition, no multidimensional scale of perceived minority threat has been developed. These oversights have significantly impeded the advancement of research testing the empirical validity and generalizability of Blalock’s premises across racial and ethnic groups.
Against this backdrop, this dissertation extends prior work by conducting three separate but interrelated studies. The first study focuses on the development and validation of a multidimensional Perceived Latino Threat Scale (PLTS). The second study investigates how the PLTS can inform the relationship between Latino context and punitive border control sentiment. The third and final study assesses the psychometrics of another multidimensional scale of perceived threat—the Perceived Black Threat Scale (PBTS), and examines the structural invariance and distinctness of the PBTS and PLTS.
Using data collected from two college samples, I relied on a variety of different methods across the three empirical studies, including confirmatory factor analyses, bivariate and partial correlation analyses, and ordinary least squares regression. Overall, the findings suggest that both the PLTS and PBTS are multidimensional constructs that are structurally invariant and empirically distinct. In addition, perceived Latino threat significantly influenced punitive border control sentiment, but did not surface as a mediating mechanism linking ethnic context to immigration attitudes. Furthermore, whereas objective Latino population context did not demonstrate significant effects on either perceived Latino threat or punitive border control sentiment, the results emphasized perceived Latino context as a key moderator in the relationship between perceived Latino threat and punitive border control sentiment. Thus, the findings support the multidimensionality of perceived threat, as well as the hypothesized link between perceived threat and punitive controls, but raises key concerns about the generalizability of Blalock’s perspective to explain the threat-control process of Latinos. Implications for theory and research are discussed.
Against this backdrop, this dissertation extends prior work by conducting three separate but interrelated studies. The first study focuses on the development and validation of a multidimensional Perceived Latino Threat Scale (PLTS). The second study investigates how the PLTS can inform the relationship between Latino context and punitive border control sentiment. The third and final study assesses the psychometrics of another multidimensional scale of perceived threat—the Perceived Black Threat Scale (PBTS), and examines the structural invariance and distinctness of the PBTS and PLTS.
Using data collected from two college samples, I relied on a variety of different methods across the three empirical studies, including confirmatory factor analyses, bivariate and partial correlation analyses, and ordinary least squares regression. Overall, the findings suggest that both the PLTS and PBTS are multidimensional constructs that are structurally invariant and empirically distinct. In addition, perceived Latino threat significantly influenced punitive border control sentiment, but did not surface as a mediating mechanism linking ethnic context to immigration attitudes. Furthermore, whereas objective Latino population context did not demonstrate significant effects on either perceived Latino threat or punitive border control sentiment, the results emphasized perceived Latino context as a key moderator in the relationship between perceived Latino threat and punitive border control sentiment. Thus, the findings support the multidimensionality of perceived threat, as well as the hypothesized link between perceived threat and punitive controls, but raises key concerns about the generalizability of Blalock’s perspective to explain the threat-control process of Latinos. Implications for theory and research are discussed.
ContributorsInfante, Arynn A (Author) / Wang, Xia (Thesis advisor) / Pardini, Dustin (Thesis advisor) / Spohn, Cassia (Committee member) / Arizona State University (Publisher)
Created2020
Description
Decay of plant litter represents an enormous pathway for carbon (C) into the atmosphere but our understanding of the mechanisms driving this process is particularly limited in drylands. While microbes are a dominant driver of litter decay in most ecosystems, their significance in drylands is not well understood and abiotic drivers such as photodegradation are commonly perceived to be more important. I assessed the significance of microbes to the decay of plant litter in the Sonoran Desert. I found that the variation in decay among 16 leaf litter types was correlated with microbial respiration rates (i.e. CO2 emission) from litter, and rates were strongly correlated with water-vapor sorption rates of litter. Water-vapor sorption during high-humidity periods activates microbes and subsequent respiration appears to be a significant decay mechanism. I also found that exposure to sunlight accelerated litter decay (i.e. photodegradation) and enhanced subsequent respiration rates of litter. The abundance of bacteria (but not fungi) on the surface of litter exposed to sunlight was strongly correlated with respiration rates, as well as litter decay, implying that exposure to sunlight facilitated activity of surface bacteria which were responsible for faster decay. I also assessed the response of respiration to temperature and moisture content (MC) of litter, as well as the relationship between relative humidity and MC. There was a peak in respiration rates between 35-40oC, and, unexpectedly, rates increased from 55 to 70oC with the highest peak at 70oC, suggesting the presence of thermophilic microbes or heat-tolerant enzymes. Respiration rates increased exponentially with MC, and MC was strongly correlated with relative humidity. I used these relationships, along with litter microclimate and C loss data to estimate the contribution of this pathway to litter C loss over 34 months. Respiration was responsible for 24% of the total C lost from litter – this represents a substantial pathway for C loss, over twice as large as the combination of thermal and photochemical abiotic emission. My findings elucidate two mechanisms that explain why microbial drivers were more significant than commonly assumed: activation of microbes via water-vapor sorption and high respiration rates at high temperatures.
ContributorsTomes, Alexander (Author) / Day, Thomas (Thesis advisor) / Garcia-Pichel, Ferran (Committee member) / Ball, Becky (Committee member) / Hall, Sharon (Committee member) / Roberson, Robert (Committee member) / Arizona State University (Publisher)
Created2020
Description
The desire to start a family is something millions of people around the globe strive to achieve. However, many factors such as the societal changes in family planning due to increasing maternal age, use of birth control, and ever-changing lifestyles have increased the number of infertility cases seen in the United States each year. Infertility can manifest as a prolonged inability to conceive, or inability to carry a pregnancy full-term. Modern advancements in the field of reproductive medicine have begun to promote the use of Assisted Reproductive Technologies (ART) to circumvent reduced fertility in both men and women. Implementation of techniques such as In Vitro Fertilization, Intracytoplasmic Sperm Injection, and Pre-Implantation Genetic Testing have allowed many couples to conceive. There is continual effort being made towards developing more effective and personalized fertility treatments. This often begins in the form of animal research—a fundamental step in biomedical research. This dissertation examines infertility as a medical condition through the characterization of normal reproductive anatomy and physiology in the introductory overview of reproduction. Specific pathologies of male and female-factor infertility are described, which necessitates the use of ARTs. The various forms of ARTs currently utilized in a clinical setting are addressed including history, preparations, and protocols for each technology. To promote continual advancement of the field, both animal studies and human trials provide fundamental stepping-stones towards the execution of new techniques and protocols. Examples of research conducted for the betterment of human reproductive medicine are explored, including an animal study conducted in mice exploring the role of tyramine in ovulation. With the development and implementation of new technologies and protocols in the field, this also unearths ethical dilemmas that further complicate the addition of new technologies in the field. Combining an extensive review in assisted reproduction, research and clinical fieldwork, this study investigates the history and development of novel research conducted in reproductive medicine and explores the broader implications of new technologies in the field.
ContributorsPeck, Shelbi Marie (Author) / Baluch, Debra P (Thesis advisor) / Maienschein, Jane (Thesis advisor) / Sweazea, Karen (Committee member) / Ellison, Karin (Committee member) / Arizona State University (Publisher)
Created2021
Description
The purpose of this study is to examine how sex influences the physical demandof weapons used in homicide. The study focuses on two research questions using data
from Newark, New Jersey: (1) Does sex influence the use of a weapon that is more or
less physically demanding to commit homicide? and (2) Does the sex dyad of the
offender and victim influence using a weapon that is more or less physically demanding?
The descriptive analysis shows significant relationships between the sex dyad of the
offender and victim in homicide and the level of physical demand used to perpetrate
homicide. The logistic multinomial regression analysis shows suspect sex and suspect
and victim sex dyads significantly predict the physical demand of the weapons used to
perpetrate homicide compared to those who utilized weapons of low physical demand.
The results support the need to challenge traditional perspectives regarding the role of sex
in criminal decision-making and seek to integrate more intersectional and gendered
explanations into neoclassical theories of criminal behavior. Theoretical implications and
future avenues of research are also discussed.
ContributorsAlvarez, Gabriel (Author) / Pizarro, Jesenia M. (Thesis advisor) / Messing, Jill T. (Committee member) / Wallace, Danielle (Committee member) / Arizona State University (Publisher)
Created2022
Description
Eusocial insect colonies have often been imagined as “superorganisms” exhibiting tight homeostasis at the colony level. However, colonies lack the tight spatial and organizational integration that many multicellular, unitary organisms exhibit. Precise regulation requires rapid feedback, which is often not possible when nestmates are distributed across space, making decisions asynchronously. Thus, one should expect poorer regulation in superorganisms than unitary organisms.Here, I investigate aspects of regulation in collective foraging behaviors that involve both slow and rapid feedback processes. In Chapter 2, I examine a tightly coupled system with near-instantaneous signaling: teams of weaver ants cooperating to transport massive prey items back to their nest. I discover that over an extreme range of scenarios—even up vertical surfaces—the efficiency per transporter remains constant. My results suggest that weaver ant colonies are maximizing their total intake rate by regulating the allocation of transporters among loads. This is an exception that “proves the rule;” the ant teams are recapitulating the physical integration of unitary organisms.
Next, I focus on a process with greater informational constraints, with loose temporal and spatial integration. In Chapter 3, I measure the ability of solitarily foraging Ectatomma ruidum colonies to balance their collection of protein and carbohydrates given different nutritional environments. Previous research has found that ant species can precisely collect a near-constant ratio between these two macronutrients, but I discover these studies were using flawed statistical approaches. By developing a quantitative measure of regulatory effect size, I show that colonies of E. ruidum are relatively insensitive to small differences in food source nutritional content, contrary to previously published claims.
In Chapter 4, I design an automated, micro-RFID ant tracking system to investigate how the foraging behavior of individuals integrates into colony-level nutrient collection. I discover that spatial fidelity to food resources, not individual specialization on particular nutrient types, best predicts individual forager behavior. These findings contradict previously published experiments that did not use rigorous quantitative measures of specialization and confounded the effects of task type and resource location.
ContributorsBurchill, Andrew Taylor (Author) / Pavlic, Theodore P (Thesis advisor) / Pratt, Stephen C (Thesis advisor) / Hölldobler, Bert (Committee member) / Cease, Arianne (Committee member) / Berman, Spring (Committee member) / Arizona State University (Publisher)
Created2022
Description
Programmed cell death plays an important role in a variety of processes that promote the survival of the host organism. Necroptosis, a form of programmed cell death, occurs through a signaling pathway involving receptor-interacting serine-threonine protein kinase 3 (RIPK3). In response to vaccinia virus infection, necroptosis is induced through DNA-induced activator of interferon (DAI), which activates RIPK3, leading to death of the cell and thereby inhibiting further viral replication in host cells. DAI also localizes into stress granules, accumulations of mRNAs that have stalled in translation due to cellular stress. The toxin arsenite, a canonical inducer of stress granule formation, was used in this project to study necroptosis. By initiating necroptosis with arsenite and vaccinia virus, this research project investigated the roles of necroptosis proteins and their potential localization into stress granules. The two aims of this research project were to determine whether stress granules are important for arsenite- and virus-induced necroptosis, and whether the proteins DAI and RIPK3 localize into stress granules. The first aim was investigated by establishing a DAI and RIPK3 expression system in U2OS cells; arsenite treatment or vaccinia virus infection was then performed on the U2OS cells as well as on U2OSΔΔG3BP1/2 cells, which are not able to form stress granules. The second aim was carried out by designing fluorescent tagging for the necroptosis proteins in order to visualize protein localization with fluorescent microscopy. The results show that arsenite induces DAI-dependent necroptosis in U2OS cells and that this arsenite-induced necroptosis likely requires stress granules. In addition, the results show that vaccinia virus induces DAI-dependent necroptosis that also likely requires stress granules in U2OS cells. Furthermore, a fluorescent RIPK3 construct was created that will allowfor future studies on protein localization during necroptosis and can be used to answer questions regarding localization of necroptosis proteins into stress granules. This project therefore contributes to a greater understanding of the roles of DAI and RIPK3 in necroptosis, as well as the roles of stress granules in necroptosis, both of which are important in research regarding viral infection and cellular stress.
ContributorsGogerty, Carolina (Author) / Jacobs, Bertram (Thesis advisor) / Langland, Jeffrey (Committee member) / Jentarra, Garilyn (Committee member) / Arizona State University (Publisher)
Created2021
Description
Police use of force is an issue that generates considerable public interest. Seeing police use of force in person or via video recordings seldom looks good for the viewer. Police must constantly be aware of their reasons for using force, the methods that they employ, and the decisions that they make in using force. Optimal results come from optimal decision-making, but analyzing whether the training that police receive leads to optimal decision-making is not a topic that has been researched often. By utilizing the martial art of Brazilian Jiu-Jitsu, the Mesa Police Department has taught their officers how to maintain broad focus of attention on their environment and more time to analytically decide how and what force modalities to employ in any given situation. This has resulted in significantly less serious physical injuries to officers and citizens and optimal decisions in critical incidents.
ContributorsSipe, Paul (Author) / Becker, David V. (Thesis advisor) / Chiou, Erin (Committee member) / Gutzwiller, Robert (Committee member) / Arizona State University (Publisher)
Created2024