ASU Electronic Theses and Dissertations
This collection includes most of the ASU Theses and Dissertations from 2011 to present. ASU Theses and Dissertations are available in downloadable PDF format; however, a small percentage of items are under embargo. Information about the dissertations/theses includes degree information, committee members, an abstract, supporting data or media.
In addition to the electronic theses found in the ASU Digital Repository, ASU Theses and Dissertations can be found in the ASU Library Catalog.
Dissertations and Theses granted by Arizona State University are archived and made available through a joint effort of the ASU Graduate College and the ASU Libraries. For more information or questions about this collection contact or visit the Digital Repository ETD Library Guide or contact the ASU Graduate College at gradformat@asu.edu.
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- All Subjects: Accordion effect
- All Subjects: plasmonic imaging
Description
Mechanical properties of cells are important in maintaining physiological functions of biological systems. Quantitative measurement and analysis of mechanical properties can help understand cellular mechanics and its functional relevance and discover physical biomarkers for diseases monitoring and therapeutics.
This dissertation presents a work to develop optical methods for studying cell mechanics which encompasses four applications. Surface plasmon resonance microscopy based optical method has been applied to image intracellular motions and cell mechanical motion. This label-free technique enables ultrafast imaging with extremely high sensitivity in detecting cell deformation. The technique was first applied to study intracellular transportation. Organelle transportation process and displacement steps of motor protein can be tracked using this method. The second application is to study heterogeneous subcellular membrane displacement induced by membrane potential (de)polarization. The application can map the amplitude and direction of cell deformation. The electromechanical coupling of mammalian cells was also observed. The third application is for imaging electrical activity in single cells with sub-millisecond resolution. This technique can fast record actions potentials and also resolve the fast initiation and propagation of electromechanical signals within single neurons. Bright-field optical imaging approach has been applied to the mechanical wave visualization that associated with action potential in the fourth application. Neuron-to-neuron viability of membrane displacement was revealed and heterogeneous subcellular response was observed.
All these works shed light on the possibility of using optical approaches to study millisecond-scale and sub-nanometer-scale mechanical motions. These studies revealed ultrafast and ultra-small mechanical motions at the cellular level, including motor protein-driven motions and electromechanical coupled motions. The observations will help understand cell mechanics and its biological functions. These optical approaches will also become powerful tools for elucidating the interplay between biological and physical functions.
This dissertation presents a work to develop optical methods for studying cell mechanics which encompasses four applications. Surface plasmon resonance microscopy based optical method has been applied to image intracellular motions and cell mechanical motion. This label-free technique enables ultrafast imaging with extremely high sensitivity in detecting cell deformation. The technique was first applied to study intracellular transportation. Organelle transportation process and displacement steps of motor protein can be tracked using this method. The second application is to study heterogeneous subcellular membrane displacement induced by membrane potential (de)polarization. The application can map the amplitude and direction of cell deformation. The electromechanical coupling of mammalian cells was also observed. The third application is for imaging electrical activity in single cells with sub-millisecond resolution. This technique can fast record actions potentials and also resolve the fast initiation and propagation of electromechanical signals within single neurons. Bright-field optical imaging approach has been applied to the mechanical wave visualization that associated with action potential in the fourth application. Neuron-to-neuron viability of membrane displacement was revealed and heterogeneous subcellular response was observed.
All these works shed light on the possibility of using optical approaches to study millisecond-scale and sub-nanometer-scale mechanical motions. These studies revealed ultrafast and ultra-small mechanical motions at the cellular level, including motor protein-driven motions and electromechanical coupled motions. The observations will help understand cell mechanics and its biological functions. These optical approaches will also become powerful tools for elucidating the interplay between biological and physical functions.
ContributorsYang, Yunze (Author) / Tao, Nongjian (Thesis advisor) / Wang, Shaopeng (Committee member) / Goryll, Michael (Committee member) / Si, Jennie (Committee member) / Arizona State University (Publisher)
Created2016
Description
Vertical take-off and landing (VTOL) systems have become a crucial component of aeronautical and commercial applications alike. Quadcopter systems are rather convenient to analyze and design controllers for, owing to symmetry in body dynamics. In this work, a quadcopter model at hover equilibrium is derived, using both high and low level control. The low level control system is designed to track reference Euler angles (roll, pitch and yaw) as shown in previous work [1],[2]. The high level control is designed to track reference X, Y, and Z axis states [3].
The objective of this paper is to model, design and simulate platooning (separation) control for a fleet of 6 quadcopter units, each comprising of high and low level control systems, using a leader-follower approach. The primary motivation of this research is to examine the ”accordion effect”, a phenomenon observed in leader-follower systems due to which positioning or spacing errors arise in follower vehicles due to sudden changes in lead vehicle velocity. It is proposed that the accordion effect occurs when lead vehicle information is not directly communicated with the rest of the
system [4][5]
.
In this paper, the effect of leader acceleration feedback is observed for the quadcopter platoon. This is performed by first designing a classical platoon controller for a nominal case, where communication within the system is purely ad-hoc (i.e from one quadcopter to it’s immediate successor in the fleet). Steady state separation/positioning errors for each member of the fleet are observed and documented during simulation. Following this analysis, lead vehicle acceleration is provided to the controller (as a feed forward term), to observe the extent of it’s effect on steady state separation, specifically along tight maneuvers. Thus the key contribution of this work is a controller that stabilizes a platoon of quadcopters in the presence of the accordion effect, when employing a leader-follower approach. The modeling shown in this paper builds on previous research to design a low costquadcopter platform, the Mark 3 copter [1]. Prior to each simulation, model nonlinearities and hardware constants are measured or derived from the Mark 3 model, in an effort to observe the working of the system in the presence of realistic hardware constraints. The system is designed in compliance with Robot Operating System (ROS) and the Micro Air Vehicle Link (MAVLINK) communication protocol.
ContributorsSrinivasan, Anshuman (Author) / Rodriguez, Armando A. (Thesis advisor) / Si, Jennie (Committee member) / Tsakalis, Konstantinos (Committee member) / Arizona State University (Publisher)
Created2021