ASU Electronic Theses and Dissertations
This collection includes most of the ASU Theses and Dissertations from 2011 to present. ASU Theses and Dissertations are available in downloadable PDF format; however, a small percentage of items are under embargo. Information about the dissertations/theses includes degree information, committee members, an abstract, supporting data or media.
In addition to the electronic theses found in the ASU Digital Repository, ASU Theses and Dissertations can be found in the ASU Library Catalog.
Dissertations and Theses granted by Arizona State University are archived and made available through a joint effort of the ASU Graduate College and the ASU Libraries. For more information or questions about this collection contact or visit the Digital Repository ETD Library Guide or contact the ASU Graduate College at gradformat@asu.edu.
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Description
Mechanical properties of cells are important in maintaining physiological functions of biological systems. Quantitative measurement and analysis of mechanical properties can help understand cellular mechanics and its functional relevance and discover physical biomarkers for diseases monitoring and therapeutics.
This dissertation presents a work to develop optical methods for studying cell mechanics which encompasses four applications. Surface plasmon resonance microscopy based optical method has been applied to image intracellular motions and cell mechanical motion. This label-free technique enables ultrafast imaging with extremely high sensitivity in detecting cell deformation. The technique was first applied to study intracellular transportation. Organelle transportation process and displacement steps of motor protein can be tracked using this method. The second application is to study heterogeneous subcellular membrane displacement induced by membrane potential (de)polarization. The application can map the amplitude and direction of cell deformation. The electromechanical coupling of mammalian cells was also observed. The third application is for imaging electrical activity in single cells with sub-millisecond resolution. This technique can fast record actions potentials and also resolve the fast initiation and propagation of electromechanical signals within single neurons. Bright-field optical imaging approach has been applied to the mechanical wave visualization that associated with action potential in the fourth application. Neuron-to-neuron viability of membrane displacement was revealed and heterogeneous subcellular response was observed.
All these works shed light on the possibility of using optical approaches to study millisecond-scale and sub-nanometer-scale mechanical motions. These studies revealed ultrafast and ultra-small mechanical motions at the cellular level, including motor protein-driven motions and electromechanical coupled motions. The observations will help understand cell mechanics and its biological functions. These optical approaches will also become powerful tools for elucidating the interplay between biological and physical functions.
This dissertation presents a work to develop optical methods for studying cell mechanics which encompasses four applications. Surface plasmon resonance microscopy based optical method has been applied to image intracellular motions and cell mechanical motion. This label-free technique enables ultrafast imaging with extremely high sensitivity in detecting cell deformation. The technique was first applied to study intracellular transportation. Organelle transportation process and displacement steps of motor protein can be tracked using this method. The second application is to study heterogeneous subcellular membrane displacement induced by membrane potential (de)polarization. The application can map the amplitude and direction of cell deformation. The electromechanical coupling of mammalian cells was also observed. The third application is for imaging electrical activity in single cells with sub-millisecond resolution. This technique can fast record actions potentials and also resolve the fast initiation and propagation of electromechanical signals within single neurons. Bright-field optical imaging approach has been applied to the mechanical wave visualization that associated with action potential in the fourth application. Neuron-to-neuron viability of membrane displacement was revealed and heterogeneous subcellular response was observed.
All these works shed light on the possibility of using optical approaches to study millisecond-scale and sub-nanometer-scale mechanical motions. These studies revealed ultrafast and ultra-small mechanical motions at the cellular level, including motor protein-driven motions and electromechanical coupled motions. The observations will help understand cell mechanics and its biological functions. These optical approaches will also become powerful tools for elucidating the interplay between biological and physical functions.
ContributorsYang, Yunze (Author) / Tao, Nongjian (Thesis advisor) / Wang, Shaopeng (Committee member) / Goryll, Michael (Committee member) / Si, Jennie (Committee member) / Arizona State University (Publisher)
Created2016
Description
This thesis addresses the design and control of three phase inverters. Such inverters are
used to produce three-phase sinusoidal voltages and currents from a DC source. They
are critical for injecting power from renewable energy sources into the grid. This is
especially true since many of these sources of energy are DC sources (e.g. solar
photovoltaic) or need to be stored in DC batteries because they are intermittent (e.g. wind
and solar). Two classes of inverters are examined in this thesis. A control-centric design
procedure is presented for each class. The first class of inverters is simple in that they
consist of three decoupled subsystems. Such inverters are characterized by no mutual
inductance between the three phases. As such, no multivariable coupling is present and
decentralized single-input single-output (SISO) control theory suffices to generate
acceptable control designs. For this class of inverters several families of controllers are
addressed in order to examine command following as well as input disturbance and noise
attenuation specifications. The goal here is to illuminate fundamental tradeoffs. Such
tradeoffs include an improvement in the in-band command following and output
disturbance attenuation versus a deterioration in out-of-band noise attenuation.
A fundamental deficiency associated with such inverters is their large size. This can be
remedied by designing a smaller core. This naturally leads to the second class of inverters
considered in this work. These inverters are characterized by significant mutual
inductances and multivariable coupling. As such, SISO control theory is generally not
adequate and multiple-input multiple-output (MIMO) theory becomes essential for
controlling these inverters.
used to produce three-phase sinusoidal voltages and currents from a DC source. They
are critical for injecting power from renewable energy sources into the grid. This is
especially true since many of these sources of energy are DC sources (e.g. solar
photovoltaic) or need to be stored in DC batteries because they are intermittent (e.g. wind
and solar). Two classes of inverters are examined in this thesis. A control-centric design
procedure is presented for each class. The first class of inverters is simple in that they
consist of three decoupled subsystems. Such inverters are characterized by no mutual
inductance between the three phases. As such, no multivariable coupling is present and
decentralized single-input single-output (SISO) control theory suffices to generate
acceptable control designs. For this class of inverters several families of controllers are
addressed in order to examine command following as well as input disturbance and noise
attenuation specifications. The goal here is to illuminate fundamental tradeoffs. Such
tradeoffs include an improvement in the in-band command following and output
disturbance attenuation versus a deterioration in out-of-band noise attenuation.
A fundamental deficiency associated with such inverters is their large size. This can be
remedied by designing a smaller core. This naturally leads to the second class of inverters
considered in this work. These inverters are characterized by significant mutual
inductances and multivariable coupling. As such, SISO control theory is generally not
adequate and multiple-input multiple-output (MIMO) theory becomes essential for
controlling these inverters.
ContributorsSarkar, Aratrik (Author) / Rodriguez, Armando A. (Thesis advisor) / Si, Jennie (Committee member) / Tsakalis, Konstantinos (Committee member) / Arizona State University (Publisher)
Created2015