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- Creators: School of Molecular Sciences
- Creators: Department of English
- Resource Type: Text
Description
The act of writing music criticism comes with numerous difficulties inherently involved in describing the ineffable qualities of audio in the medium of the written word. My music criticism podcast, Here and Now with Steve Jozef, offers a method of reviewing albums that sidesteps these problems by interweaving the criticism with clips of the songs discussed. This format holds the critic accountable to root their opinion in tangible components in the music, and makes clear the direct line between the qualities of the music at hand and the reviewer's verdict on that music. I wrote, recorded, edited, and distributed 13 episodes of the podcast over the course of six months; I've selected four of those episodes to represent my work for the purposes of the thesis. Their scripts, as well as links to the full episodes, are included with this paper. In addition, I thoroughly researched modern academic thought on the nature, purpose, and future of popular music criticism. This research helped me refine a conception of my ideal theoretical audience for the podcast, forced me to confront and assess my music evaluation criteria through various lenses, and develop the goal of creating a pattern of empathetic listening amongst my listeners. Over the course of developing the project, I ran into several obstacles in using copyrighted material on the show without permission from the copyright holders. Thus the podcast also became a case study in evaluating modern copyright law and its application, and demonstrating what needs to change about both for music criticism to sustain in this form.
ContributorsJozef, Steven James (Author) / Wells, Christopher (Thesis director) / Duerden, Sarah (Committee member) / Tinapple, David (Committee member) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Breast cancer is the leading cause of cancer-related deaths of women in the united states. Traditionally, Breast cancer is predominantly treated by a combination of surgery, chemotherapy, and radiation therapy. However, due to the significant negative side effects associated with these traditional treatments, there has been substantial efforts to develop alternative therapies to treat cancer. One such alternative therapy is a peptide-based therapeutic cancer vaccine. Therapeutic cancer vaccines enhance an individual's immune response to a specific tumor. They are capable of doing this through artificial activation of tumor specific CTLs (Cytotoxic T Lymphocytes). However, in order to artificially activate tumor specific CTLs, a patient must be treated with immunogenic epitopes derived from their specific cancer type. We have identified that the tumor associated antigen, TPD52, is an ideal target for a therapeutic cancer vaccine. This designation was due to the overexpression of TPD52 in a variety of different cancer types. In order to start the development of a therapeutic cancer vaccine for TPD52-related cancers, we have devised a two-step strategy. First, we plan to create a list of potential TPD52 epitopes by using epitope binding and processing prediction tools. Second, we plan to attempt to experimentally identify MHC class I TPD52 epitopes in vitro. We identified 942 potential 9 and 10 amino acid epitopes for the HLAs A1, A2, A3, A11, A24, B07, B27, B35, B44. These epitopes were predicted by using a combination of 3 binding prediction tools and 2 processing prediction tools. From these 942 potential epitopes, we selected the top 50 epitopes ranked by a combination of binding and processing scores. Due to the promiscuity of some predicted epitopes for multiple HLAs, we ordered 38 synthetic epitopes from the list of the top 50 epitope. We also performed a frequency analysis of the TPD52 protein sequence and identified 3 high volume regions of high epitope production. After the epitope predictions were completed, we proceeded to attempt to experimentally detected presented TPD52 epitopes. First, we successful transduced parental K562 cells with TPD52. After transduction, we started the optimization process for the immunoprecipitation protocol. The optimization of the immunoprecipitation protocol proved to be more difficult than originally believed and was the main reason that we were unable to progress past the transduction of the parental cells. However, we believe that we have identified the issues and will be able to complete the experiment in the coming months.
ContributorsWilson, Eric Andrew (Author) / Anderson, Karen (Thesis director) / Borges, Chad (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Transient Receptor Potential (TRP) ion channels are a diverse family of nonselective, polymodal sensors in uni- and multicellular eukaryotes that are implicated in an assortment of biological contexts and human disease. The cold-activated TRP Melastatin-8 (TRPM8) channel, also recognized as the human body's primary cold sensor, is among the few TRP channels responsible for thermosensing. Despite sustained interest in the channel, the mechanisms underlying TRPM8 activation, modulation, and gating have proved challenging to study and remain poorly understood. In this thesis, I offer data collected on various expression, extraction, and purification conditions tested in E. Coli expression systems with the aim to optimize the generation of a structurally stable and functional human TRPM8 pore domain (S5 and S6) construct for application in structural biology studies. These studies, including the biophysical technique nuclear magnetic spectroscopy (NMR), among others, will be essential for elucidating the role of the TRPM8 pore domain in in regulating ligand binding, channel gating, ion selectively, and thermal sensitivity. Moreover, in the second half of this thesis, I discuss the ligation-independent megaprimer PCR of whole-plasmids (MEGAWHOP PCR) cloning technique, and how it was used to generate chimeras between TRPM8 and its nearest analog TRPM2. I review steps taken to optimize the efficiency of MEGAWHOP PCR and the implications and unique applications of this novel methodology for advancing recombinant DNA technology. I lastly present preliminary electrophysiological data on the chimeras, employed to isolate and study the functional contributions of each individual transmembrane helix (S1-S6) to TRPM8 menthol activation. These studies show the utility of the TRPM8\u2014TRPM2 chimeras for dissecting function of TRP channels. The average current traces analyzed thus far indicate that the S2 and S3 helices appear to play an important role in TRPM8 menthol modulation because the TRPM8[M2S2] and TRPM8[M2S3] chimeras significantly reduce channel conductance in the presence of menthol. The TRPM8[M2S4] chimera, oppositely, increases channel conductance, implying that the S4 helix in native TRPM8 may suppress menthol modulation. Overall, these findings show that there is promise in the techniques chosen to identify specific regions of TRPM8 crucial to menthol activation, though the methods chosen to study the TRPM8 pore independent from the whole channel may need to be reevaluated. Further experiments will be necessary to refine TRPM8 pore solubilization and purification before structural studies can proceed, and the electrophysiology traces observed for the chimeras will need to be further verified and evaluated for consistency and physiological significance.
ContributorsWaris, Maryam Siddika (Author) / Van Horn, Wade (Thesis director) / Redding, Kevin (Committee member) / School of Molecular Sciences (Contributor) / Department of English (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Musical interpretation is challenging when one's goal is to evoke an emotional response from an audience. In order to develop a well-informed interpretation of Mozart's Fantasia in D minor K. 397, a study was conducted on the historical background of the piece and various performances by well-regarded performers. Fantasias are written works, but improvisatory by nature. Mozart's fantasias were influenced by C. P. E. Bach's, which included sudden changes in emotion. An Emil Gilels performance provided a classically trained approach, while Mitsuko Uchida's performance provided an emotional approach. Colin Tilney and John Irving performances elucidated the sound of the instruments that Mozart would have been composing with. Altogether, the research culminated in an interpretation of the D minor Fantasia that endeavored to capture the essence of fantasy, improvisation and emotion.
ContributorsMo, Gina Nan (Author) / Emmery, Laura (Thesis director) / Creviston, Hannah (Committee member) / Department of Psychology (Contributor) / School of International Letters and Cultures (Contributor) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Volunteer motivation and satisfaction were assessed at Project CURE, a nonprofit that collects, sorts, tests, and ships donated medical supplies and equipment to healthcare facilities in developing countries. This research was the result of a yearlong partnership between the Community Action Research Experiences (CARE) program and Project CURE. Volunteers at Project CURE were surveyed (N=147) after completing a volunteer session to assess their motivation for volunteering, satisfaction with their experience, and any recommendations they had for improving the volunteer program. Five categories of motivating factors were assessed and it was found that the Values and Understanding categories were the strongest motivating factors. Overall, volunteers rated their experience highly, but the results indicated a number of small changes that Project CURE could make to better meet volunteers' needs, and better communicate the impact of volunteers' work, which could pave the way to increases in the numbers of volunteer hours Project CURE receives and increased quality of volunteer work.
ContributorsStepanek, Rachel (Author) / Reesing, Amy (Thesis director) / Dumka, Larry (Committee member) / School of Molecular Sciences (Contributor) / School of Human Evolution and Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
The purpose of this study was to create an evaluation plan for the Preventing Abusive Conduct (PAC/Peace) program, within the community-based organization Chrysalis. PAC/Peace is a program that provides treatment for domestic abuse offenders through psycho-educational counseling groups. This study analyzed evaluation methods used by similar programs to determine which methods were successful, as well as what criteria were used to evaluate these programs. A literature review was conducted on similar studies to find which measures should be used to determine whether a treatment program met the desired goals for its participants. The literature review, along with interviews with Chrysalis staff were used as resources to create a cost-effective evaluation plan for Chrysalis to measure the effectiveness of their offender treatment program. The results indicated a two part evaluation plan would be the most beneficial for Chrysalis at this time. The first part consists of official reports of recidivism collected at one year, and three years post-graduation from the program. The second is utilization of The Revised Conflict Tactics Scale as an offender self-reporting measure, administered at program intake and exit, as well as one year post-graduation. When implemented, the results of this evaluation will give Chrysalis insight to which aspects of the treatment are working, and which aspects might be improved upon. The results acquired from the evaluation may also help PAC/Peace to become an evidence-based program.
ContributorsScouras, Natasha Elexa (Author) / Low, Sabina (Thesis director) / Dumka, Larry (Committee member) / School of Social Transformation (Contributor) / School of Molecular Sciences (Contributor) / School of Human Evolution and Social Change (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Netflix has positioned itself at the forefront of the future of television with its original programming, which has been rolled out in greater and more frequent amounts just in the last couple of years. The streaming service has already experimented with creativity in ways most other shows and creators haven't, playing with the pacing of overall seasons as well as the length of episodes. So, too, Netflix has been at the forefront of increasing visibility for minority characters on television. Many of its shows incorporate racially diverse casts and depict lots of LGBTQ characters, a refreshingly realistic view of the world that many of its viewers have always lived in but haven't yet witnessed on television. Visibility and representation are critical concepts for analyzing minority characters on television. It is important for diverse characters to be seen, first and foremost, but also to be seen in positive or at least realistic lights. Care must be taken to avoid fulfilling stereotypes or tropes, and attention must be paid to what has happened to other characters who have come before. However, many of Netflix's portrayals of these characters, particularly bisexual characters, leave much to be desired. With the original dramas House of Cards, Hemlock Grove, Orange is the New Black, and Sense8, all of which include characters who identify as or behave bisexually, Netflix has been reluctant to use the specific word bisexual to describe characters, and many don't even identify their sexuality with a synonym for the term. Many of the bisexual characters that I identified died or were killed on the shows, and nearly all of them fulfilled stereotypes or tropes in some way. There were multiple scenes of threesomes or other distinctly kinky sexual encounters, which served to exoticize bisexuality and distance it from the more normatively viewed identities of heterosexuality and homosexuality. Ultimately, while Netflix's original programming has offered increased visibility to bisexual characters, it has yet to reflect the real community it seeks to portray. In particular, Netflix's refusal to label characters as bisexual is frustrating and limiting. It can be argued that this is a progressive move toward more ideas of sexual fluidity and a post-modern lack of sexual labels, but there are not enough depictions of identified bisexual characters on television yet for this to make sense. Until bisexual characters and their identities are not invisibilized or stigmatized, more work has to be done to ensure that bisexual people are represented fairly and accurately on television and in all media.
ContributorsKoerth, Kimberly Marie (Author) / Himberg, Julia (Thesis director) / Sturges, Robert (Committee member) / Department of English (Contributor) / College of Public Service and Community Solutions (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / School of Social Transformation (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
We hypothesized that recurrent exposure to a temporal discounting task would habitize participants, so that they become insensitive to framing effects. Temporal discounting is a behavioral trend which describes how people discount the value of a reward dependent on the time until receipt. Participants completed a temporal discounting task weekly for five weeks, to promote formation of a habitual decision strategy. Concomitant with this, we expected that people would shift their decision process from a deliberate, goal-oriented approach that is sensitive to changes in reward outcomes and environmental context, to a simplified, automatic approach that minimizes cognitive effort. We expected that this shift in decision strategy would be evident in a reduced influence of contextual effects on choice outcomes. We tested this hypothesis by leveraging two framing effects \u2014 the date/delay effect and the decimal effect. Consistent with our hypothesis, we find that the date/delay effect is significant on week 1, shows significant changes in week 1 to week 5, and is no longer significant on week 5. The results for the decimal effects were not significant. We discuss these results with respect to the cognitive processes that underlie temporal discounting and self-control.
ContributorsSt Amand, Jesse Dean (Author) / McClure, Samuel (Thesis director) / Sanabria, Federico (Committee member) / School of Molecular Sciences (Contributor) / Department of Physics (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
This is a constructed language made for fictional creatures that will be featured in a novel. The creatures are feral goblins, and they are based on Irish mythology. The novel that the goblins and the language will be featured in is titled MaddConn, and it was written by Jon Bendera. It is an urban fantasy novel. The goblins have a language in the novel because there are a number of goblin characters throughout the story. One of the main characters, Maddi, has to survive amongst the feral goblins for a portion of the novel while another main character, Connor, works his way to her. She has multiple close encounters with them, and also has to find her way around their territory, so she comes into contact with the language many times. Although the language in the story was created to have developed prior to humanity, this constructed language was made to look somewhat similar to human language. As it will be involved in fiction that will be read by humans, it needs to be easily understood by humans. The language reveals features of the goblin culture and anatomy. For instance, it can be seen in the language that their shallow, wide mouths and thin lips influence their phonology. Their physiology influences much of their language. Likewise, they have three fingers on each hand, which influences their number system. As the goblins and their language developed before humans and because they are not human creatures, the typology and word order do not follow all of the correlations found in human languages. All in all, the lexicon includes many words that have to do with three of their favorite things: violence, food, and work.
ContributorsMartinez, Randi Nicole (Author) / Van Gelderen, Elly (Thesis director) / Maring, Heather (Committee member) / Schreyer, Christine (Committee member) / Department of English (Contributor, Contributor, Contributor) / Barrett, The Honors College (Contributor)
Created2017-05
Description
Orbiting space debris is an active issue that affects the capability of space launch for future satellites, probes, and space shuttles, and it will become a nearly insurmountable problem without action. Debris of varying sizes and speeds orbit the Earth at a range of heights above the atmosphere and need to be removed to avoid damage to crucial equipment of active orbiting satellites including the International Space Station. Finding a feasible solution to space debris removal requires that several facets be covered to become a reality; these include being aware of the problem in magnitude and source. This literature assessment covers the magnitude of space debris in low-earth and geosynchronous orbit as well as collision events which have increased the amount of space debris. There have been efforts made by several space agencies to control the amount of space debris added to orbit by current and future launches over the last decade \u2014 serving as a temporary fix before removal can be executed. This paper explores known removal efforts through mitigation, projects conceived and tested by DARPA, related space policies and laws, CubeSat technology, and the cataloguing of known space debris. To make space debris removal a reality, roadblocks need to be removed to acquire permission from states or countries for space missions. For example, these restrictions are in place to protect the assets of several countries and organizations. Guidelines set to curb the growth of space debris fail to prevent the growth due to the restrictions for ownership rights making them not as effective. This paper covers space policy and laws, the economy, satellite ownership, international conflict, status of space debris, and the overall feasibility of space debris removal. It will then discuss currently proposed solutions for the removal of space debris. Finally, this paper attempts to weight the advantages and disadvantages of the idea that space debris removal should include the opportunity to recycle materials. For example, defunct satellites and other discarded space crafts could be used for future launches. It will conclude with a personal exploration of what materials can be recycled, what chemical processes can be used to break down materials, and how to combine recycling and chemical processes for space-based recycling stations between Earth and the moon. The overall question that drives the search for making space debris removal a reality is whether it is feasible in multiple areas including technologically, legally, monetarily, and physically.
ContributorsBreden, Elizabeth Catherine (Author) / Foy, Joseph (Thesis director) / Thoesen, Andrew (Committee member) / Maximon, Leonard (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2017-05