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This 15-week long course is designed to introduce students, specifically in Arizona, to basic sustainability and conservation principles in the context of local reptile wildlife. Throughout the course, the students work on identifying the problem, creating visions for the desired future, and finally developing a strategy to help with reptile species survival in the valley. Research shows that animals in the classroom have led to improved academic success for students. Thus, through creating this course I was able to combine conservation and sustainability curriculum with real-life animals whose survival is directly being affected in the valley. My hope is that this course will help students identify a newfound passion and call to action to protect native wildlife. The more awareness and actionable knowledge which can be brought to students in Arizona about challenges to species survival the more likely we are to see a change in the future and a stronger sense of urgency for protecting wildlife. In order to accomplish these goals, the curriculum was developed to begin with basic concepts of species needs such as food and shelter and basic principles of sustainability. As the course progresses the students analyze current challenges reptile wildlife faces, like urban sprawl, and explore options to address these challenges. The course concludes with a pilot pitch where students present their solution projects to the school.
As climate change continues to escalate natural hazards around the globe, certain communities feel the impacts of these disasters more so than others. After Hurricane Maria devastated communities in 2017, Puerto Rico struggled to respond to the needs of its citizens, particularly those in rural areas. Many of the regions affected did not have resilient community structures in place to be able to withstand the systemic ripple effects of the hurricane. However, various community endeavors have developed post-Hurricane Maria to foster community collaboration and resiliency, including the development of agricultural tourism, otherwise known as agritourism. <br/>Although agritourism has begun to develop in rural regions of Puerto Rico, including the municipalities of Utuado, Ciales, Florida, and Jayuya, a systems-understanding is lacking of the current agritourism situation in the region and its related capacities, limitations, and opportunities of agritourism. To address this gap, a spatially explicit understanding and map of the underlying tourism infrastructure is needed to support the development of sustainable agritourism in Utuado, Jayuya, Ciales, and Florida municipalities in Puerto Rico. <br/>This report spatially represents the current state of tourism opportunities in the region as a result of asking “What are the spatial networks of gastronomy, accommodations, farms, and attractions that support the development of agritourism in Utuado, Jayuya, Ciales and Florida municipalities in Puerto Rico?” Three steps lead to the spatial representation starting with developing a comprehensive inventory. Second, we visualize the spatial map through Google Maps. Lastly, we explore the larger context of the report through an ArcGIS Storymap. The inventory will help with better understanding the number and variety of tourism resources available. The spatial visualization will help with understanding the distribution of resources and explore potential connections between resources and what relationships could be fostered in the future. Lastly, the ArcGIS Storymap will serve as a framework for outlining the future development of the SARE project. Overall, this report outlines the spatial maps of tourism resources and provides a tool to be used by community partners, tourists, and project partners.
The synergistic effects between Vorinostat and Tamoxifen observed through a phase II study on breast cancer patients resistant to hormone therapy may involve more than the modulation of ER-alpha to reverse Tamoxifen resistance in ERBC cells. RT-qPCR of genes expressed in Tamoxifen resistant cells, trefoil factor 1(TFF1) and v-myc avian myelocytomatosis viral oncogene homolog (MYC), were evaluated along with ESR1 and Diablo as a control. MYC was observed to have increased expression in the treated cells, whereas the other genes had a decrease in their expression levels after the cells were treated for 3 days with Vorinostat IC30 of 1 µM. As for targeting the AR, MCF7 Tamoxifen sensitive and resistant cells were not affected by the AR antagonists to determine an IC50. The cell viability for all MCF7 sub-clones only decreased for high concentrations of 5.56 µM - 50 µM in Bicalutamide and 16.67 µM – 50 µM of MDV1300. Furthermore, hormone depletion of MCF7 G11 Tamoxifen resistant sub-clones did not show a great response to DHT stimulation or the AR antagonists. In the RT-qPCR, the MCF7 G11 cells showed an increase in mRNA expression for ER, AR, and PR after 4 hours of treatment with estradiol. As for the DHT treatment, ER, AR, PR, and PSA had a minimal increase in the fold change, but the fold change in AR was less than in the estradiol treatment. The Mayo Clinic will investigate the possible usage of AR as a biomarker through immunohistochemistry.