The evolution of cooperation is a fundamental problem in biology, especially for non-relatives, where indirect fitness benefits cannot counter within-group inequalities. Multilevel selection models show how cooperation can evolve if it generates a group-level advantage, even when cooperators are disadvantaged within their group. This allows the possibility of group selection, but few examples have been described in nature. Here we show that group selection can explain the evolution of cooperative nest founding in the harvester ant Pogonomyrmex californicus. Through most of this species’ range, colonies are founded by single queens, but in some populations nests are instead founded by cooperative groups of unrelated queens. In mixed groups of cooperative and single-founding queens, we found that aggressive individuals had a survival advantage within their nest, but foundress groups with such non-cooperators died out more often than those with only cooperative members. An agent-based model shows that the between-group advantage of the cooperative phenotype drives it to fixation, despite its within-group disadvantage, but only when population density is high enough to make between-group competition intense. Field data show higher nest density in a population where cooperative founding is common, consistent with greater density driving the evolution of cooperative foundation through group selection.

General ecological thought pertaining to plant biology, conservation, and urban areas has rested on two potentially contradictory underlying assumptions. The first is that non-native plants can spread easily from human developments to “pristine” areas. The second is that native plants cannot disperse through developed areas. Both assume anthropogenic changes to ecosystems create conditions that favor non-native plants and hinder native species. However, it is just as likely that anthropogenic alterations of habitats will favor certain groups of plant species with similar functional traits, whether native or not. Migration of plants can be divided into the following stages: dispersal, germination, establishment, reproduction and spread. Functional traits of species determine which are most successful at each of the stages of invasion or range enlargement. I studied the traits that allow both native and non-native plant species to disperse into freeway corridors, germinate, establish, reproduce, and then disperse along those corridors in Phoenix, Arizona. Field methods included seed bank sample collection and germination, vegetation surveys, and seed trapping. I also evaluated concentrations of plant-available nitrate as a result of localized nitrogen deposition. While many plant species found on the roadsides are either landscape varieties or typical weedy species, some uncommon native species and unexpected non-native species were also encountered. Maintenance regimes greatly influence the amount of vegetative cover and species composition along roadsides. Understanding which traits permit success at various stages of the invasion process indicates whether it is native, non-native, or species with particular traits that are likely to move through the city and establish in the desert. In a related case study conducted in Victoria, Australia, transportation professionals and ecologists were surveyed regarding preferences for roadside landscape design. Roadside design and maintenance projects are typically influenced by different groups of transportation professionals at various stages in a linear project cycle. Landscape architects and design professionals have distinct preferences and priorities compared to other transportation professionals and trained ecologists. The case study reveals the need for collaboration throughout the stages of design, construction and maintenance in order to efficiently manage roadsides for multiple priorities.