Colorimetric assays are an important tool in point-of-care testing that offers several advantages to traditional testing methods such as rapid response times and inexpensive costs. A factor that currently limits the portability and accessibility of these assays are methods that can objectively determine the results of these assays. Current solutions consist of creating a test reader that standardizes the conditions the strip is under before being measured in some way. However, this increases the cost and decreases the portability of these assays. The focus of this study is to create a machine learning algorithm that can objectively determine results of colorimetric assays under varying conditions. To ensure the flexibility of a model to several types of colorimetric assays, three models were trained on the same convolutional neural network with different datasets. The images these models are trained on consist of positive and negative images of ETG, fentanyl, and HPV Antibodies test strips taken under different lighting and background conditions. A fourth model is trained on an image set composed of all three strip types. The results from these models show it is able to predict positive and negative results to a high level of accuracy.

Although relatively new technology, machine learning has rapidly demonstrated its many uses. One potential application of machine learning is the diagnosis of ailments in medical imaging. Ideally, through classification methods, a computer program would be able to identify different medical conditions when provided with an X-ray or other such scan. This would be very beneficial for overworked doctors, and could act as a potential crutch to aid in giving accurate diagnoses. For this thesis project, five different machine-learning algorithms were tested on two datasets containing 5,856 lung X-ray scans labeled as either “Pneumonia” or “Normal”. The goal was to determine which algorithm achieved the highest accuracy, as well as how preprocessing the data affected the accuracy of the models. The following supervised-learning methods were tested: support vector machines, logistic regression, decision trees, random forest, and a convolutional neural network. Each model was adjusted independently in order to achieve maximum performance before accuracy metrics were generated to pit the models against each other. Additionally, the effect of resizing images on model performance was investigated. Overall, a convolutional neural network proved to be the superior model for pneumonia detection, with a 91% accuracy. After resizing to 28x28, CNN accuracy decreased to 85%. The random forest model performed second best. The 28x28 PneumoniaMNIST dataset achieved higher accuracy using traditional machine learning models than the HD Chest X-Ray dataset. Resizing the Chest X-ray images had minimal effect on traditional model performance when resized to 28x28 or larger.