To date, it has been difficult to elucidate the role of tau in learning and memory during adulthood due to developmental compensation of other microtubule associated proteins in Tau knockout (KO) mice. Here, we generated an adeno-associated virus (AAV) expressing a doxycycline (doxy)-inducible short-hairpin (sh) RNA targeted to tau, and…
To date, it has been difficult to elucidate the role of tau in learning and memory during adulthood due to developmental compensation of other microtubule associated proteins in Tau knockout (KO) mice. Here, we generated an adeno-associated virus (AAV) expressing a doxycycline (doxy)-inducible short-hairpin (sh) RNA targeted to tau, and stereotaxically and bilaterally injected 7-month-old C57BL/6 mice with either the AAV-shRNAtau or an AAV expressing a scramble shRNA sequence. Seven days after the injections, all animals were administered doxy for thirty-five days to induce expression of shRNAs, after which they were tested in the open field, rotarod and Morris water maze (MWM) to assess anxiety like behavior, motor coordination and spatial reference memory, respectively. Our results show that reducing tau in the adult hippocampus produces significant impairments in motor coordination, endurance and spatial memory. Tissue analyses shows that tau knockdown reduces hippocampal dendritic spine density and the levels of BDNF and synaptophysin, two proteins involved in memory formation and plasticity. Our approach circumvents the developmental compensation issues observed in Tau KO models and shows that reducing tau levels during adulthood impairs cognition.
Does a consistent mentor help a youth transition into a secure and independent adult life? To answer this, I have used a grounded theory methodology to research elements of the foster care system. Through academic research, I audited the landscape of mentoring within foster care and then helped in the…
Does a consistent mentor help a youth transition into a secure and independent adult life? To answer this, I have used a grounded theory methodology to research elements of the foster care system. Through academic research, I audited the landscape of mentoring within foster care and then helped in the designing of a framework for a foster youth mentoring service could look like and have researched the areas in which a mentor can benefit a youth. I have listened to and recorded the stories of three college students with lived foster care experience so that I could learn from those who have firsthand experience. After using these methods, the findings showed that a consistent mentor figure is a key factor in the transition into a secure and independent adult life.
