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Attitudes and habits are extremely resistant to change, but a disruption of the magnitude of the COVID-19 pandemic has the potential to bring long-term, massive societal changes. During the pandemic, people are being compelled to experience new ways of interacting, working, learning, shopping, traveling, and eating meals. Going forward, a critical question is whether these experiences will result in changed behaviors and preferences in the long term. This paper presents initial findings on the likelihood of long-term changes in telework, daily travel, restaurant patronage, and air travel based on survey data collected from adults in the United States in Spring 2020. These data suggest that a sizable fraction of the increase in telework and decreases in both business air travel and restaurant patronage are likely here to stay. As for daily travel modes, public transit may not fully recover its pre-pandemic ridership levels, but many of our respondents are planning to bike and walk more than they used to. These data reflect the responses of a sample that is higher income and more highly educated than the US population. The response of these particular groups to the COVID-19 pandemic is perhaps especially important to understand, however, because their consumption patterns give them a large influence on many sectors of the economy.
Universities host a large, young and diverse population that commutes to the same location every day, which makes them ideally suited for public transportation ridership. However, at many universities in the US, this potential for high levels of transit ridership is not being maximized. This research aims to identify the areas where Valley Metro’s public transit service to ASU’s Tempe campus is over- and under-performing in comparison with the overall public transportation service to the entire Phoenix metro area. The hypothesis states that proximity to campus and the convenience of using public transportation would be the two main factors in determining the success of an area’s public transportation service. ASU’s Parking & Transit Services provided confidential data with the addresses of all the students and employees who purchased a parking pass, transit pass and bike registration. With these data, the public transportation mode share for commuters to ASU in each census block group was calculated and compared to the mode share for the general public, which was based on US Census data. The difference between the public transit mode shares of ASU pass holders vs. commuting by the general public was then computed and analyzed to identify areas as hot and cold spots. These heat maps are then compared to the hypothesized factors of proximity to campus and the convenience of public transportation in terms of the light rail line, park-and-ride lots, and number of transfers needed to connect to campus. The transfers were estimated using origin and destination survey data provided by Valley Metro. Results show that the convenience of public transportation was a driving factor in explaining where the transit mode share to ASU is higher than that of the general public, whereas the proximity to campus had little impact on the areas with high ASU-specific transit mode shares. There is an absence of hot spots directly around the campus which is explained by the combination of both high transit share for the non-ASU population and the large share of ASU students and employees using active transportation and free circulator buses this close to campus. These findings are significant specifically to ASU because the university can learn where the transit service is performing well and where it is underperforming. Using these findings, ASU PTS can adjust its pricing, policies, services and infrastructure and work with Valley Metro and the City of Tempe to improve the ridership for both students and employees. Future research can compare more factors to further interpret what leads to success for transit service to university campuses.
This dissertation aims to 1) develop new strategy to identify high affinity nucleic acid aptamers against biological ligand; and 2) explore highly orthogonal RNA riboregulators in vivo for constructing multi-input gene circuits with NOT logic. With the aid of a DNA nanoscaffold, pairs of hetero-bivalent aptamers for human alpha thrombin were identified with ultra-high binding affinity in femtomolar range with displaying potent biological modulations for the enzyme activity. The newly identified bivalent aptamers enriched the aptamer tool box for future therapeutic applications in hemostasis, and also the strategy can be potentially developed for other target molecules. Secondly, by employing a three-way junction structure in the riboregulator structure through de-novo design, we identified a family of high-performance RNA-sensing translational repressors that down-regulates gene translation in response to cognate RNAs with remarkable dynamic range and orthogonality. Harnessing the 3WJ repressors as modular parts, we integrate them into biological circuits that execute universal NAND and NOR logic with up to four independent RNA inputs in Escherichia coli.
Fluorescent labeling is of paramount importance to biological studies of proteins. For the development of new extrinsic small fluorophores, a series of tryptophan analogues has been designed and synthesized. Their pdCpA derivatives have been synthesized for tRNA activation and in vitro protein synthesis. The photophysical properties of the tryptophan (Trp) analogues have been examined, some of which can be selectively monitored even in the presence of multiple native tryptophan residues. Further, some of the Trp analogues form efficient FRET pairs with acceptors such as acridon-2-ylalanine (Acd) or L-(7-hydroxycoumarin-4-yl)ethylglycine (HCO) for the selective study of conformational changes in proteins.
Molecules which can bind with high sequence selectivity to a chosen target in a gene sequence are of interest for the development of gene therapy, diagnostic devices for genetic analysis, and as molecular tools for nucleic acid manipulations. Stereoselective synthesis of different alanyl nucleobase amino acids is described. Their pdCpA derivatives have been synthesized for tRNA activation and site-specific incorporation into the DNA-binding protein RRM1 of hnRNP LL. It is proposed that the nucleobase moieties in the protein may specifically recognize base sequence in the i-motif DNA through H-bonding and base-stacking interactions.
The mitochondrial respiratory chain accumulates more oxidative damage than any other organelle within the cell. Dysfunction of this organelle is believed to drive the progression of many diseases, thus mitochondria are an important potential drug target. Reactive oxygen species (ROS) are generated when electrons from the respiratory chain escape and interact with oxygen. ROS can react with proteins, lipids or DNA causing cell death. For the development of effective neuroprotective drugs, a series of N-hydroxy-4-pyridones have been designed and synthesized as CoQ10 analogues. All the analogues synthesized were evaluated for their ability to quench lipid peroxidation and reactive oxygen species (ROS).
especially given the increasing numbers of residents choosing to bike and walk. Sharing
the roads with automobiles, these alternative road users are particularly vulnerable to
sustain serious injuries. With this in mind, it is important to identify the factors that
influence the severity of bicyclist and pedestrian injuries in automobile collisions. This
study uses traffic collision data gathered from California Highway Patrol’s Statewide
Integrated Traffic Records System (SWITRS) to predict the most important
determinants of injury severity, given that a collision has occurred. Multivariate binomial
logistic regression models were created for both pedestrian and bicyclist collisions, with
bicyclist/pedestrian/driver characteristics and built environment characteristics used as
the independent variables. Results suggest that bicycle infrastructure is not an important
predictor of bicyclist injury severity, but instead bicyclist age, race, sobriety, and speed
played significant roles. Pedestrian injuries were influenced by pedestrian and driver age
and sobriety, crosswalk use, speed limit, and the type of vehicle at fault in the collision.
Understanding these key determinants that lead to severe and fatal injuries can help
local communities implement appropriate safety measures for their most susceptible
road users.