Matching Items (84)
Description
Anaerobic Digestion (AD) typically stabilizes 40-60% of influent wastewater sludge. Improving the methane yield in wastewater may produce enough energy to power some wastewater treatment processes, while the production of volatile-fatty acids (VFAs) generates economic incentives for yard waste pre-fermentation. In this research, pre-fermenters consisting of inocula composed of media; cellulose, lantana, or grass; and rabbit cecotrope were fed various concentrations of plant matter. The contents of these pre-fermenters were the influent for respective anaerobic digesters. The microbial consortium derived for the lignocellulosic pretreatment with common yard waste in Arizona successfully increased methane production in AD, while producing additional VFAs during pretreatment in all systems. The performance of the system appeared to depend on plant matter loading and operating time, with a higher plant loading increasing the VFA production and a longer operating time increasing soluble chemical oxygen demand (COD) in pre-fermentation, and therefore the methane production in AD increased. The pre-fermenter with the highest plant matter loading and longest operating time –1.44 g plant matter per day at a 9.6% influent concentration and 193 days of total operating time– produced 10,000 mg COD/L of VFA, and its reactor produced about 460 mL methane (CH4) per day, which was almost twice the production of the control AD at 250 mL CH4 per day. This research uses yard waste that would previously be disposed of in landfill to increase valuable product production in AD. The potential value added to wastewater treatment plant (WWTP) processes by these methods could incentivize the expansion of wastewater treatment, thereby increasing sanitation access. The use of net-neutral biogas as a fuel source for WWTPs is additionally an incremental solution for reducing carbon equivalents present in the atmosphere, thereby reducing the greenhouse gas effect.
ContributorsPittman, Smith (Author) / Rittmann, Bruce (Thesis director) / Young, Michelle (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / School of Sustainable Engineering & Built Envirnmt (Contributor)
Created2022-05
Description
While most household surfactants are biodegradable in aerobic conditions, their presence in a microbiological treatment process can lead to the proliferation of antimicrobial-resistance genes (ARG) in bacteria, such as Pseudomonas aeruginosa. Surfactants can be cationic, anionic, or zwitterionic, and these different classes may have different effects on the proliferation of ARG. This study evaluated how the three classes of surfactants affected the microbial community’s structure and ARG in O2-based membrane biofilm reactors (O2-MBfRs) that provided at least 98% surfactant removal. Cationic cetrimonium bromide (CTAB) had by far the strongest impact with highest ARG abundance in the biofilm. In particular, Pseudomonas and Stenotrophomonas, the two main genera in the biofilm treating CTAB, were highly correlated to the abundance of ARG for efflux pumps and antibiotic inactivation. CTAB also promoted potential of horizontal gene transfer (HGT) of ARG. Combining results from the metabolome and metagenome identified four possible pathways for CTAB biodegradation. Of special important is a new pathway: β-carbon oxidation of CTAB to produce betaine. An insufficient nitrogen source could lead to irreversible ARB and ARG enrichment in the MBfR biofilm. Finally, a two-stage O2-MBfR successfully removed a high concentration (730 mg/L) of CTAB: Partial CTAB removal in the Lead reactor relieved inhibition in the Lag reactor. Metagenomic analysis also revealed that the Lag reactor was enriched in genes for CTAB and metabolite oxygenation.
ContributorsZheng, Chenwei (Author) / Rittmann, Bruce (Thesis advisor) / Delgado, Anca (Committee member) / Krajmalnik-Brown, Rosa (Committee member) / Lai, Yen-Jung (Committee member) / Arizona State University (Publisher)
Created2023
Description
Due to the use of fertilizers, concentrations of harmful nitrate have increased in groundwater and surface waters globally in the last century. Water treatment plants primarily use separation techniques for nitrate treatment, but these technologies create a high nitrate concentration brine that is costly to dispose of. This dissertation focuses on catalytic hydrogenation, an emerging technology capable of reducing nitrate to nitrogen gas using hydrogen gas (H2). This technology reduces nitrate at rates >95% and is an improvement over technologies used at water treatment plants, because the nitrate is chemically transformed with harmless byproducts and no nitrate brine. The goal of this dissertation is to upgrade the maturity of catalytic nitrate hydrogenation systems by overcoming several barriers hindering the scale-up of this technology. Objective 1 is to compare different methods of attaching the bimetallic catalyst to a hollow-fiber membrane surface to find a method that results in 1) minimized catalyst loss, and 2) repeatable nitrate removal over several cycles. Results showed that the In-Situ MCfR-H2 deposition was successful in reducing nitrate at a rate of 1.1 min-1gPd-1 and lost less than 0.05% of attached Pd and In cumulatively over three nitrate treatment cycles. Objective 2 is to synthesize catalyst-films with varied In3+ precursor decorated over a Pd0 surface to show the technology can 1) reliably synthesize In-Pd catalyst-films with varied bimetallic ratios, and 2) optimize nitrate removal activity by varying In-Pd ratio. Results showed that nitrate removal activity was optimized with a rate constant of 0.190 mg*min-1L-1 using a catalyst-film with a 0.045 In-Pd ratio. Objective 3 is to perform nitrate reduction in a continuous flow reactor for two months to determine if nitrate removal activity can be sustained over extended operation and identify methods to overcome catalyst deactivation. Results showed that a combination of increased hydraulic residence time and reduced pH was successful in increasing the nitrate removal and decreasing harmful nitrite byproduct selectivity to 0%. These objectives increased the technology readiness of this technology by enabling the reuse of the catalyst, maximizing nitrate reduction activity, and achieving long-term nitrate removal.
ContributorsLevi, Juliana (Author) / Westerhoff, Paul (Thesis advisor) / Rittmann, Bruce (Thesis advisor) / Garcia-Segura, Sergi (Committee member) / Wong, Michael (Committee member) / Lind Thomas, Mary Laura (Committee member) / Emady, Heather (Committee member) / Arizona State University (Publisher)
Created2023
Description
In order to optimize the ability of Geobacter sulfurreducens to produce electrical current and remediate wastewater, several physiological challenges must be overcome. The accumulation of protons at the electrode surface of a microbial fuel cell (MFC) decreases the pH, and, thus, the ability of the bacteria to maintain baseline metabolic conditions. To evaluate the extent to which this pH change hinders performance, the buffer concentration supplied to G. sulfurreducens reactors was varied. The resulting biofilms were subjected to chronoamperometry, cyclic voltammetry, and confocal microscopy to determine metabolic function and biofilm thickness. Biofilms grown with a 30-mM bicarbonate buffer experienced limitations on cell function and current output due to proton accumulation, while 90- and 150-mM conditions alleviated these limitations most of the measurements. Based on the current output, estimated biofilm thickness, and the medium-rate and slow-rate scan rate cyclic voltammetry, benefits exist for buffer concentrations greater than 30 mM. If the kinetics of G. sulfurreducens electron transfer are optimized, the potential of the technique to be implemented for energy recovery is improved.
ContributorsCoulam, Jordan (Author) / Torres, Cesar (Thesis advisor) / Delgado, Anca (Committee member) / Rittmann, Bruce (Committee member) / Arizona State University (Publisher)
Created2024
Description
The dissimilatory reduction of nitrate, or denitrification, offers the potential of a sustainable, cost effective method for the non-disruptive mitigation of earthquake-induced soil liquefaction. Worldwide, trillions of dollars of infrastructure are at risk for liquefaction damage in earthquake prone regions. However, most techniques for remediating liquefiable soils are either not applicable to sites near existing infrastructure, or are prohibitively expensive. Recently, laboratory studies have shown the potential for biogeotechnical soil improvement techniques such as microbially induced carbonate precipitation (MICP) to mitigate liquefaction potential in a non-disruptive manner. Multiple microbial processes have been identified for MICP, but only two have been extensively studied. Ureolysis, the most commonly studied process for MICP, has been shown to quickly and efficiently induce carbonate precipitation on particle surfaces and at particle contacts to improve the stiffness, strength, and dilatant behavior of liquefiable soils. However, ureolysis also produces copious amounts of ammonium, a potentially toxic byproduct. The second process studied for MICP, denitrification, has been shown to precipitate carbonate, and hence improve soil properties, much more slowly than ureolysis. However, the byproducts of denitrification, nitrogen and carbon dioxide gas, are non-toxic, and present the added benefit of rapidly desaturating the treated soil. Small amounts of desaturation have been shown to increase the cyclic resistance, and hence the liquefaction resistance, of liquefiable soils. So, denitrification offers the potential to mitigate liquefaction as a two-stage process, with desaturation providing short term mitigation, and MICP providing long term liquefaction resistance. This study presents the results of soil testing, stoichiometric modeling, and microbial ecology characterization to better characterize the potential use of denitrification as a two-stage process for liquefaction mitigation.
ContributorsO'Donnell, Sean (Author) / Kavazanjian, Edward (Thesis advisor) / Rittmann, Bruce (Committee member) / Houston, Sandra (Committee member) / Arizona State University (Publisher)
Created2016
Description
N-nitrosodimethylamine (NDMA) is a probable human carcinogen and drinking water disinfection by-product. NDMA forms as the product of reactions between chloramines and precursor compounds in water. This dissertation aims to provide insight into the removal of NDMA precursors, their nature, and a method to aid in their identification. Watershed-derived precursors accounted for more of and greater variability to NDMA formation upon chloramination than polymer-derived precursors in environmental samples. Coagulation polymers are quaternary amines, which have low NDMA yield but high use rates. Watershed-derived precursors were removed up to 90% by sorption to activated carbon, but activated carbon exhibited much less (<10%) sorption of polymer-derived precursors. Combined with literature NDMA molar yields of model anthropogenic compounds, where anthropogenic chemicals in some cases have NDMA yields >90% and biological compounds always have yields <2%, trace, organic, amine containing, anthropogenic chemicals were implicated as the most likely source of NDMA precursors in the watershed. Although activated carbon removes these precursors well, identification of individual compounds may result in more cost effective mitigation strategies. Therefore, I developed a method to isolate NDMA precursors from other organic matter into methanol to facilitate their identification. Optimization of the method resulted in a median recovery of NDMA precursors of 82% from 10 surface waters and one wastewater. The method produces 1,000X concentrated NDMA precursors and, in collaboration with the University of Colorado Center for Environmental Mass Spectrometry, time of flight mass spectrometry (TOF-MS) was performed on multiple treated wastewater and raw drinking water isolates. During TOF-MS, tertiary amines can cleave to form a neutral loss and an R group ion that is dependent on the original structure and I wrote a software program to “trawl” exported TOF-MS spectra for the diagnostic neutral loss resulting from fragmentation of tertiary amines. Methadone was identified as one new NDMA precursor that occurs at concentrations that form physiologically relevant levels of NDMA in surface water and wastewater. The approach used here to identify NDMA precursors is adaptable to other unknown disinfection by-product precursors given that a functional group is known that can 1)control sorption and 2)produce a predictable diagnostic fragment.
ContributorsHanigan, David (Author) / Westerhoff, Paul (Thesis advisor) / Rittmann, Bruce (Committee member) / Herckes, Pierre (Committee member) / Arizona State University (Publisher)
Created2015
Description
Microbial electrochemical cells (MXCs) offer an alternative to methane production in anaerobic water treatment and the recapture of energy in waste waters. MXCs use anode respiring bacteria (ARB) to oxidize organic compounds and generate electrical current. In both anaerobic digestion and MXCs, an anaerobic food web connects the metabolisms of different microorganisms, using hydrolysis, fermentation and either methanogenesis or anode respiration to break down organic compounds, convert them to acetate and hydrogen, and then convert those intermediates into either methane or current. In this dissertation, understanding and managing the interactions among fermenters, methanogens, and ARB were critical to making developments in MXCs. Deep sequencing technologies were used in order to identify key community members, understand their role in the community, and identify selective pressures that drove the structure of microbial communities. This work goes from developing ARB communities by finding and using the best partners to managing ARB communities with undesirable partners. First, the foundation of MXCs, namely the ARB they rely on, was expanded by identifying novel ARB, the genus Geoalkalibacter, and demonstrating the presence of ARB in 7 out of 13 different environmental samples. Second, a new microbial community which converted butyrate to electricity at ~70% Coulombic efficiency was assembled and demonstrated that mixed communities can be used to assemble efficient ARB communities. Third, varying the concentrations of sugars and ethanol fed to methanogenic communities showed how increasing ED concentration drove decreases in methane production and increases in both fatty acids and the propionate producing genera Bacteroides and Clostridium. Finally, methanogenic batch cultures, fed glucose and sucrose, and exposed to 0.15 – 6 g N-NH4+ L-1 showed that increased NH4+ inhibited methane production, drove fatty acid and lactate production, and enriched Lactobacillales (up to 40% abundance) above 4 g N-NH4+ L-1. Further, 4 g N-NH4+ L-1 improved Coulombic efficiencies in MXCs fed with glucose and sucrose, and showed that MXC communities, especially the biofilm, are more resilient to high NH4+ than comparable methanogenic communities. These developments offer new opportunities for MXC applications, guidance for efficient operation of MXCs, and insights into fermentative microbial communities.
ContributorsMiceli, Joseph (Author) / Torres, César I (Thesis advisor) / Krajmalnik-Brown, Rosa (Thesis advisor) / Rittmann, Bruce (Committee member) / Arizona State University (Publisher)
Created2015
Description
To date, the production of algal biofuels is not economically sustainable due to the cost of production and the low cost of conventional fuels. As a result, interest has been shifting to high value products in the algae community to make up for the low economic potential of algal biofuels. The economic potential of high-value products does not however, eliminate the need to consider the environmental impacts. The majority of the environmental impacts associated with algal biofuels overlap with algal bioproducts in general (high-energy dewatering) due to the similarities in their production pathways. Selecting appropriate product sets is a critical step in the commercialization of algal biorefineries.
This thesis evaluates the potential of algae multiproduct biorefineries for the production of fuel and high-value products to be economically self-sufficient and still contribute to climate change mandates laid out by the government via the Energy Independence and Security Act (EISA) of 2007. This research demonstrates:
1) The environmental impacts of algal omega-3 fatty acid production can be lower than conventional omega-3 fatty acid production, depending on the dewatering strategy.
2) The production of high-value products can support biofuels with both products being sold at prices comparable to 2016 prices.
3) There is a tradeoff between revenue and fuel production
4) There is a tradeoff between the net energy ratio of the algal biorefinery and the economic viability due to the lower fuel production in a multi-product model that produces high-value products and diesel vs. the lower economic potential from a multi-product model that just produces diesel.
This work represents the first efforts to use life cycle assessment and techno-economic analysis to assess the economic and environmental sustainability of an existing pilot-scale biorefinery tasked with the production of high-value products and biofuels. This thesis also identifies improvements for multiproduct algal biorefineries that will achieve environmentally sustainable biofuel and products while maintaining economic viability.
This thesis evaluates the potential of algae multiproduct biorefineries for the production of fuel and high-value products to be economically self-sufficient and still contribute to climate change mandates laid out by the government via the Energy Independence and Security Act (EISA) of 2007. This research demonstrates:
1) The environmental impacts of algal omega-3 fatty acid production can be lower than conventional omega-3 fatty acid production, depending on the dewatering strategy.
2) The production of high-value products can support biofuels with both products being sold at prices comparable to 2016 prices.
3) There is a tradeoff between revenue and fuel production
4) There is a tradeoff between the net energy ratio of the algal biorefinery and the economic viability due to the lower fuel production in a multi-product model that produces high-value products and diesel vs. the lower economic potential from a multi-product model that just produces diesel.
This work represents the first efforts to use life cycle assessment and techno-economic analysis to assess the economic and environmental sustainability of an existing pilot-scale biorefinery tasked with the production of high-value products and biofuels. This thesis also identifies improvements for multiproduct algal biorefineries that will achieve environmentally sustainable biofuel and products while maintaining economic viability.
ContributorsBarr, William James (Author) / Landis, Amy E. (Thesis advisor) / Westerhoff, Paul (Thesis advisor) / Rittmann, Bruce (Committee member) / Khanna, Vikas (Committee member) / Arizona State University (Publisher)
Created2016
Description
The production and applications of engineered nanomaterials (ENM) has increased rapidly in the last decade, with release of ENM to the environment through the sewer system and municipal wastewater treatment plants (WWTPs) being of concern. Currently, the literature on ENM release from WWTPs and removal of ENM by WWTPs is insufficient and disorganized. There is little quantitative data on the removal of multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO), or few-layer graphene (FLG), from wastewater onto biomass. The removal of pristine and oxidized MWCNTs (O-MWCNTs), graphene oxide (GO), few-layer graphene (FLG) and Tween™ 20-coated Ag ENM by the interaction with biomass were determined by programmable thermal analysis (PTA) and UV-Vis spectrophotometry. The removal of pristine and O-MWCNTs was 96% from the water phase via aggregation and 30-min settling in presence or absence of biomass with an initial MWCNT concentration of 25 mg/L. The removal of 25 mg/L GO was 65% with biomass concentration at or above 1,000 mg TSS/L. The removal of 1 mg/L FLG was 16% with 50 mg TSS/L. The removal of Tween™ 20 Ag ENM with concentration from 0.97 mg/L to 2.6 mg/L was from 11% to 92% with biomass concentration of 500 mg TSS/L to 3,000 mg TSS/L, respectively.
A database of ENM removal by biomass was established by analyzing data from published papers, and non-linear solid-liquid distribution functions were built into the database. A conventional activated sludge (CAS) model was built based on a membrane bioreactor (MBR) model from a previous paper. An iterative numeric approach was adapted to the CAS model to calculate the result of non-linear adsorption of ENM by biomass in the CAS process. Kinetic studies of the CAS model showed the model performance changed mostly in the first 10 days after changing influent chemical oxygen demand (COD) concentration, and reached a steady state after 11 days. Over 60% of ENMs which have distribution coefficients in the database reached higher than 50% removal by the CAS model under general operational conditions. This result suggests that traditional WWTP which include the CAS process can remove many known types of ENMs in certain degree.
A database of ENM removal by biomass was established by analyzing data from published papers, and non-linear solid-liquid distribution functions were built into the database. A conventional activated sludge (CAS) model was built based on a membrane bioreactor (MBR) model from a previous paper. An iterative numeric approach was adapted to the CAS model to calculate the result of non-linear adsorption of ENM by biomass in the CAS process. Kinetic studies of the CAS model showed the model performance changed mostly in the first 10 days after changing influent chemical oxygen demand (COD) concentration, and reached a steady state after 11 days. Over 60% of ENMs which have distribution coefficients in the database reached higher than 50% removal by the CAS model under general operational conditions. This result suggests that traditional WWTP which include the CAS process can remove many known types of ENMs in certain degree.
ContributorsYu, Zhicheng (Author) / Westerhoff, Paul (Thesis advisor) / Rittmann, Bruce (Committee member) / Herckes, Pierre (Committee member) / Arizona State University (Publisher)
Created2015
Description
The unicellular cyanobacterium Synechocystis sp. PCC 6803 contains a NiFe-type bidirectional hydrogenase that is capable of using reducing equivalents to reduce protons and generate H¬2. In order to achieve sustained H2 production using this cyanobacterium many challenges need to be overcome. Reported H2 production from Synechocystis is of low rate and often transient. Results described in this dissertation show that the hydrogenase activity in Synechocystis is quite different during periods of darkness and light. In darkness, the hydrogenase enzyme acts in a truly bidirectional way and a particular H2 concentration is reached that depends upon the amount of biomass involved in H2 production. On the other hand, in the presence of light the enzyme shows only transient H2 production followed by a rapid and constitutive H2 oxidation. H2 oxidation and production were measured from a variety of Synechocystis strains in which components of the photosynthetic or respiratory electron transport chain were either deleted or inhibited. It was shown that the light-induced H2 oxidation is dependent on the activity of cytochrome b6f and photosystem I but not on the activity of photosystem II, indicating a channeling of electrons through cytochrome b6f and photosystem I. Because of the sequence similarities between subunits of NADH dehydrogenase I in E. coli and subunits of hydrogenase in Synechocystis, NADH dehydrogenase I was considered as the most likely candidate to mediate the electron transfer from hydrogenase to the membrane electron carrier plastoquinone, and a three-dimensional homology model with the associated subunits shows that structurally it is possible for the subunits of the two complexes to assemble. Finally, with the aim of improving the rate of H2 production in Synechocystis by using a powerful hydrogenase enzyme, a mutant strain of Synechocystis was created in which the native hydrogenase was replaced with the hydrogenase from Lyngbya aestuarii BL J, a strain with higher capacity for H2 production. H2 production was detected in this Synechocystis mutant strain, but only in the presence of external reductants. Overall, this study emphasizes the importance of redox partners in determining the direction of H2 flux in Synechocystis.
ContributorsDatta, Īpsitā (Author) / Vermaas, Willem Fj (Thesis advisor) / Garcia-Pichel, Ferran (Committee member) / Rittmann, Bruce (Committee member) / Jones, Anne K (Committee member) / Arizona State University (Publisher)
Created2015