This thesis project is part of a larger collaboration documenting the history of the ASU Biodesign Clinical Testing Laboratory (ABCTL). There are many different aspects that need to be considered when transforming to a clinical testing laboratory. This includes the different types of tests performed in the laboratory. In addition to the diagnostic polymerase chain reaction (PCR) test that is performed detecting the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), antibody testing is also performed in clinical laboratories. Antibody testing is used to detect a previous infection. Antibodies are produced as part of the immune response against SARS-CoV-2. There are many different forms of antibody tests and their sensitives and specificities have been examined and reviewed in the literature. Antibody testing can be used to determine the seroprevalence of the disease which can inform policy decisions regarding public health strategies. The results from antibody testing can also be used for creating new therapeutics like vaccines. The ABCTL recognizes the shifting need of the community to begin testing for previous infections of SARS-CoV-2 and is developing new forms of antibody testing that can meet them.

The purpose of this research is to exploit the neglect of specific populations and diseases in Latin America through an epidemiological literature review. As a small part of a larger publication, the foci of this research was the infectious disease, helminthiasis. Using manually indexed abstracts from the National Library of Medicine database in PubMed, 4,594 papers were synthesized and then processed for further review. Of those papers, 29 provided information about helminths in indigenous populations. These papers were reviewed and used in prevalence data extraction and variable analysis. The main conclusion was to reveal the fact that from an entire health database less than 30 papers provided information about the persistence of helminths in indigenous communities of Latin America. Not only that but the few papers that could be analyzed had consistently high prevalence ratios.

This study attempts to answer the following questions: Is civic engagement a social activity among 18-25-year-old college students? How are opinions regarding civic and political engagement impacted by social settings? How are civic and political engagement atmospheres impacted by social distancing and isolation protocol? In this study, the researcher hypothesized that civic and political engagement are social activities, so they are therefore susceptible to changing social context. Since the COVID-19 pandemic disrupted typical social interaction through social distancing and isolation protocol, the researcher hypothesized that it also altered mechanisms of civic and political engagement. Political engagement would be more prevalent among students who participate with others even in pandemic conditions that may otherwise decrease close contact and social interactions. These findings seem to disagree with the literature that suggests young people are supplanting voting with other forms of engagement (Zukin et al., 2006). Rather, the “complexity” denoted in interviews and in reports of engagements on the pre- and post-election surveys suggests that young people are voting as well as dedicating their time to other activities. Voting does seem to be a social activity according to the interviews, poll observations, and the surveys. This is consistent with the literature regarding social norms and group predictors. However, this social aspect of engagement seems to manifest in a wider variety of formats that originally thought. Finally, students continued to engage in the context of the pandemic that surrounded the election in question. It seems that the formats through which students engaged have expanded to maintain the connections that are crucial to civic participation.

With the passage of the Affordable Care Act, the health system in the United States is now being further challenged. There is bipartisan debate on how it can be reconstructed: one party states that the government plays too big of a role, while the other believes it plays too little. Regardless, Americans want change. Reconstruction is not a new topic by any means, and other countries have been forced to do so due to political violence. This paper explores the history and current healthcare organizations of Japan, Iraq, and Afghanistan. These countries have all encountered major political turmoil, which has led to the rebuilding of their respective healthcare systems. Though the United States is not facing political violence that will necessitate reorganization, the examination of nations that have been forced to do so offers lessons applicable to the healthcare system in the US.

The SARS-CoV-2 (Covid-19) virus has had severe impacts on college students' ways of life. To examine how students were coping and perceiving the Covid-19 pandemic, a secondary analysis of an online survey across the three Arizona public universities investigated students’ knowledge about Covid-19, engagement with preventive strategies, pandemic preparedness and gauged their risk-perception. Results from our analysis indicate that the students were knowledgeable about Covid-19 and were changing their habits and engaging with preventive measures. Results further suggest that students were prepared for the pandemic in terms of resources and were exhibiting high-risk perceptions. The data also revealed that students who were being cautious and engaging with preventive behaviors had a higher risk-perception than individuals who were not. As for individuals who were prepared for the pandemic in terms of supplies, their risk perception was similar to those who did not have supplies. Individuals who were prepared and capable of providing a single caretaker to tend to their sick household members and isolate them in a separate room had a higher risk perception than those who could not. These results can help describe how college students will react to a future significant event, what resources students may be in need of, and how universities can take additional steps to keep their students safe and healthy. The results from this study and recommendations will provide for a stronger and more understanding campus community during times of distress and can improve upon already established university protocols for health crises and even natural disasters.

The heat treatment of lithic raw material is a globally dispersed technology that improves the flaking quality of toolstone. While not all types of stone respond to heat treatment, many forms of microcrystalline silicates do, including jasper. Here, we aim to better understand how Perkinsville jasper responds to heat treatment. Perkinsville jasper occurs in the Perkinsville Valley of Yavapai County, Arizona, and was utilized prehistorically by the Prescott, Sinagua, and Hohokam cultures. For our study, we collected seven boulders of jasper from private land (with permission) in Yavapai County. These boulders were flintknapped into 74 spalls which were subsequently heated in an electric kiln using 20 treatment protocols with systematically varying combinations of maximum temperature and maximum heating times. Afterward, we compared multiple quantitative and qualitative characteristics of unheated and heated flakes taken from the same nodule pre- and post-heat treatment. Our heating protocol allows us to determine an ‘optimal heating context’ for Perkinsville jasper and to better understand how variation in time and temperature influences flaking quality of the stone. Lastly, this research develops an experimental reference dataset that can be used by other researchers studying raw material use and heat treatment in the Southwest United States.

With the passage of the Affordable Care Act, the health system in the United States is now being further challenged. There is bipartisan debate on how it can be reconstructed: one party states that the government plays too big of a role, while the other believes it plays too little. Regardless, Americans want change. Reconstruction is not a new topic by any means, and other countries have been forced to do so due to political violence. This paper explores the history and current healthcare organizations of Japan, Iraq, and Afghanistan. These countries have all encountered major political turmoil, which has led to the rebuilding of their respective healthcare systems. Though the United States is not facing political violence that will necessitate reorganization, the examination of nations that have been forced to do so offers lessons applicable to the healthcare system in the US.

Bermuda Land Snails make up a genus called Poecilozonites that is endemic to Bermuda and is extensively present in its fossil record. These snails were also integral to the creation of the theory of punctuated equilibrium. The DNA of mollusks is difficult to sequence because of a class of proteins called mucopolysaccharides that are present in high concentrations in mollusk tissue, and are not removed with standard DNA extraction methods. They inhibit Polymerase Chain Reactions (PCRs) and interfere with Next Generation Sequencing methods. This paper will discuss the DNA extraction methods that were designed to remove the inhibitory proteins that were tested on another gastropod species (Pomacea canaliculata). These were chosen because they are invasive and while they are not pulmonates, they are similar enough to Bermuda Land Snails to reliably test extraction methods. The methods that were tested included two commercially available kits: the Qiagen Blood and Tissue Kit and the Omega Biotek Mollusc Extraction Kit, and one Hexadecyltrimethylammonium Bromide (CTAB) Extraction method that was modified for use on mollusk tissue. The Blood and Tissue kit produced some DNA, the mollusk kit produced almost none, and the CTAB Extraction Method produced the highest concentrations on average, and may prove to be the most viable option for future extractions. PCRs attempted with the extracted DNA have all failed, though it is likely due to an issue with reagents. Further spectrographic analysis of the DNA from the test extractions has shown that they were successful at removing mucopolysaccharides. When the protocol is optimized, it will be used to extract DNA from the tissue from six individuals from each of the two extant species of Bermuda Land Snails. This DNA will be used in several experiments involving Next Generation Sequencing, with the goal of assembling a variety of genome data. These data will then be used to a construct reference genome for Bermuda Land Snails. The genomes generated by this project will be used in population genetic analyses between individuals of the same species, and between individuals of different species. These analyses will then be used to aid in conservation efforts for the species.

Media witnessing and storytelling for environmental justice (EJ) provide an avenue to understand the relationships between “multiple realities of environmental injury” and to analyze “fleeting phenomena with lasting form; thereby transforming phenomena that are experienced in a plurality of lives into publicly recognized history” (Houston, 2012, 419, 422). This creates opportunities to challenge and eradicate the oppressive structures that deem certain individuals and groups disposable and ultimately protect the possessive investment in whiteness. Therefore, for the purposes of EJ, media witnessing creates space for dynamic, citizen-based storytelling which can undermine narratives that promote the life versus economy framework that has perpetuated oppression, injustice, and state sanctioned violence. Media witnessing in an EJ context demonstrates the potential for collective understanding and action, political opportunities, and healing.<br/>This paper is an analysis of the process of media witnessing in regards to the Flint Water Crisis and the construction of the Dakota Access Pipeline (DAPL) and will apply an EJ lens to this phenomenon. It will discuss how media witnessing in response to these two crises can be used as a precedent for understanding and utilizing this framework and digital storytelling to address the crises of 2020, primarily the COVID-19 pandemic and racial injustice. It will then examine how the intersectionality of race, gender, and age has implications for future media witnessing and storytelling in the context of EJ movements. Finally, it will explain how media witnessing can motivate holistic policymaking in the favor of EJ initiatives and the health and wellbeing of all Americans, as well as how such policymaking and initiatives must acknowledge the double-edged sword that is social media.

The COVID-19 pandemic places significant strain on the U.S. healthcare system due to the high number of coronavirus cases. During the pandemic, there was much unknown about the virus, its course of the disease, COVID-19 diagnosis, treatments, or other imperative information needed to contain the virus. Resources within the healthcare system, such as PPE and healthcare workers, were in short supply and exacerbated the difficulty of managing the viral outbreak. Peer-reviewed articles suggest that telehealth, the application of electronic information and telecommunication technologies in healthcare, proved useful in public health and clinical care during the 2020 public health emergency due to a novel virus. The scoping review broadly assessed themes of telehealth’s strengths and weaknesses during the COVID-19 pandemic. These findings could suggest how virtual medicine may be a helpful tool to improve access in addition to the quality of care in the future of medicine. Assessments of case studies suggest that telehealth helped provide care to large patient volumes by aiding with communication, data collection, triage, remote patient monitoring, and critical care. Limitations of expanding telehealth subsequent to the pandemic include, but not limited to, a lack of national standards for practice and restrictions of utility for certain populations. Populations may include those with low socioeconomic status, specific cultural practices, and beliefs, or physical and cognitive ability barriers. Outlining the benefits and limitations of telehealth may suggest how virtual medicine can provide valuable in day-to-day medical practices and other pathogenic outbreaks.