Methods: We have designed a multiplexed magnetics programmable bead ELISA (MagProBE) to profile the immune responses of the proteins from 11 high-risk HPV types and 2 low-risk types—106 genes in total. HPV genes were optimized for human expression and either built with PCR or commercially purchased, and cloned into the Gateway-compatible pANT7_cGST vector for in vitro transcription/translation (IVTT) in a MagProBE array. Anti-GST antibody (Ab) labeling was then used to measure gene expression.
Results: 53/106 (50%) HPV genes have been cloned and tested for expression of protein. 91% of HPV proteins expressed at levels above the background control (MFI = 2288), and the mean expression was MFI = 4318. Codon-optimized genes have also shown a 20% higher expression over non-codon optimized genes.
Conclusion: Although this research is ongoing, it suggests that gene optimization may improve IVTT expression of HPV proteins in human HeLa lysate. Once the remaining HPV proteins have been expression confirmed, the cDNA for each gene will be printed onto slides and tested in serologic assays to identify potential Ab biomarkers to CIN3.
Managing a work-home balance is a daunting task for any parent. It is often difficult to take leave from work to care for one’s family due to financial barriers, which simultaneously poses a threat to family development. Although many countries have parental leave policies in place to account for this, effectiveness of these policies vary by country. This study aims to find to what extent parental leave has an impact on the quality of life. In this study, quality of life was investigated by the rank of the country on the Happiness Index and through the lens of achieving sustainable family development, which was subsequently described to be reflected by a country’s governmental resources provided during parental leave, as well as the country’s Gender Inequality Index. Through a cross-cultural review of literature, it was found that there seems to be an indirect, complex correlation of parental leave to the quality of life, and external factors such as sociocultural ideals, gender inequality, and varying workplace practices have greater significance on quality of life.
The SARS-CoV-2 (Covid-19) virus has had severe impacts on college students' ways of life. To examine how students were coping and perceiving the Covid-19 pandemic, a secondary analysis of an online survey across the three Arizona public universities investigated students’ knowledge about Covid-19, engagement with preventive strategies, pandemic preparedness and gauged their risk perception. Results from our analysis indicate that the students were knowledgeable about Covid-19 and were changing their habits and engaging with preventive measures. Results further suggest that students were prepared for the pandemic in terms of resources and were exhibiting high-risk perceptions. The data also revealed that students who were being cautious and engaging with preventive behaviors had a higher risk-perception than individuals who were not. As for individuals who were prepared for the pandemic in terms of supplies, their risk perception was similar to those who did not have supplies. Individuals who were prepared and capable of providing a single caretaker to tend to their sick household members and isolate them in a separate room had a higher risk perception than those who could not. These results can help describe how college students will react to a future significant event, what resources students may be in need of, and how universities can take additional steps to keep their students safe and healthy. The results from this study and recommendations will provide for a stronger and more understanding campus community during times of distress and can improve upon already established university protocols for health crises and even natural disasters.
This paper will cover a variety of stable isotope systems, both light and heavy, that are used to interpret isotopic analysis in two different disciplines: bioarchaeology and forensic anthropology. To begin, I will give short histories of both bioarchaeology and forensic anthropology, including what is considered to be the beginning of the disciplines as well as the founders of said disciplines. Following the histories of the disciplines, there will be a short background in isotopes and isotopic analysis, including an introduction to isoscapes and how isotopic data can be collected for further interpretation. There will then be an introduction to light isotopes, focusing on the ones used for this thesis, which will lead into the background of each light isotope. Following the light isotopes is an introduction to the heavy isotopes and the backgrounds of each of the heavy isotopes. Finally, this thesis will end in the conclusions section.
Natural gas development in the Northern Appalachian region has skyrocketed dramatically over the past decade. Correspondingly to the unprecedented growth rate of the natural gas industry, population health risks have shifted dramatically in response to both aerial and water pollution. With energy as a key input in all sectors of Appalachian life, the Pennsylvania region serves as a fascinating case study where clusters of unconventional gas drilling wells intersect varying population densities and governing laws to create different levels of health risks. Studies have found that horizontal hydraulic fracking corresponds to an increased risk of upper respiratory symptoms (URS), low birth weights, premature births, and certain cancers (White et al., 2009). Also, zoning and local planning laws are policy tools local governments can use to directly influence community wellbeing (Diez-Roux, 2011). This study will focus on the spatial relationship between upper respiratory symptoms (URS), a key volatile health benchmark, and the zoning/planning laws that the Oil and Natural Gas Industry must adhere to. Our project seeks to provide a preliminary understanding of the interplay between different natural gas zoning laws and the resulting health implication risks that appear in the Marcellus shale region of Pennsylvania. This is necessary to appropriately regulate and monitor hydraulic fracking. To get a better understanding of this phenomenon, spatial autocorrelation and analysis of variance statistics are integrated to generate a surface-level understanding of areas impacted by natural gas development. To guide the creation of our models, we geographically process the unconventional well locations, upper respiratory symptom health utilization, and zoning law data to develop insights that policymakers can take into consideration. Regionally, natural gas has become an integrated part of the energy sector and a driver of local economic development. The patterns drawn from this assessment provide a novel way of understanding the population health risks posed by different zoning ordinance models.