Matching Items (62)
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This study examines the impact of spatial landscape configuration (e.g., clustered, dispersed) on land-surface temperatures (LST) over Phoenix, Arizona, and Las Vegas, Nevada, USA. We classified detailed land-cover types via object-based image analysis (OBIA) using Geoeye-1 at 3-m resolution (Las Vegas) and QuickBird at 2.4-m resolution (Phoenix). Spatial autocorrelation (local

This study examines the impact of spatial landscape configuration (e.g., clustered, dispersed) on land-surface temperatures (LST) over Phoenix, Arizona, and Las Vegas, Nevada, USA. We classified detailed land-cover types via object-based image analysis (OBIA) using Geoeye-1 at 3-m resolution (Las Vegas) and QuickBird at 2.4-m resolution (Phoenix). Spatial autocorrelation (local Moran’s I ) was then used to test for spatial dependence and to determine how clustered or dispersed points were arranged. Next, we used Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) data acquired over Phoenix (daytime on 10 June and nighttime on 17 October 2011) and Las Vegas (daytime on 6 July and nighttime on 27 August 2005) to examine day- and nighttime LST with regard to the spatial arrangement of anthropogenic and vegetation features. Local Moran’s I values of each land-cover type were spatially correlated to surface temperature. The spatial configuration of grass and trees shows strong negative correlations with LST, implying that clustered vegetation lowers surface temperatures more effectively. In contrast, clustered spatial arrangements of anthropogenic land-cover types, especially impervious surfaces and open soil, elevate LST. These findings suggest that city planners and managers should, where possible, incorporate clustered grass and trees to disperse unmanaged soil and paved surfaces, and fill open unmanaged soil with vegetation. Our findings are in line with national efforts to augment and strengthen green infrastructure, complete streets, parking management, and transit-oriented development practices, and reduce sprawling, unwalkable housing development.

ContributorsMyint, Soe Win (Author) / Zheng, Baojuan (Author) / Talen, Emily (Author) / Fan, Chao (Author) / Kaplan, Shari (Author) / Middel, Ariane (Author) / Smith, Martin (Author) / Huang, Huei-Ping (Author) / Brazel, Anthony J. (Author)
Created2015-06-29
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This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface

This study seeks to determine the role of land architecture—the composition and configuration of land cover—as well as cadastral/demographic/economic factors on land surface temperature (LST) and the surface urban heat island effect of Phoenix, Arizona. It employs 1 m National Agricultural Imagery Program data of land-cover with 120mLandsat-derived land surface temperature, decomposed to 30 m, a new measure of configuration, the normalized moment of inertia, and U.S. Census data to address the question for two randomly selected samples comprising 523 and 545 residential neighborhoods (census blocks) in the city. The results indicate that, contrary to most other studies, land configuration has a stronger influence on LST than land composition. In addition, both land configuration and architecture combined with cadastral, demographic, and economic variables, capture a significant amount of explained variance in LST. The results indicate that attention to land architecture in the development of or reshaping of neighborhoods may ameliorate the summer extremes in LST.

ContributorsLi, Xiaoxiao (Author) / Li, Wenwen (Author) / Middel, Ariane (Author) / Harlan, Sharon L. (Author) / Brazel, Anthony J. (Author) / Turner II, B. L. (Author)
Created2015-12-29
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Objectives: To provide novel quantification and advanced measurements of surface temperatures (Ts) in playgrounds, employing multiple scales of data, and provide insight into hot-hazard mitigation techniques and designs for improved environmental and public health.

Methods: We conduct an analysis of Ts in two Metro-Phoenix playgrounds at three scales: neighborhood (1 km

Objectives: To provide novel quantification and advanced measurements of surface temperatures (Ts) in playgrounds, employing multiple scales of data, and provide insight into hot-hazard mitigation techniques and designs for improved environmental and public health.

Methods: We conduct an analysis of Ts in two Metro-Phoenix playgrounds at three scales: neighborhood (1 km resolution), microscale (6.8 m resolution), and touch-scale (1 cm resolution). Data were derived from two sources: airborne remote sensing (neighborhood and microscale) and in situ (playground site) infrared Ts (touch-scale). Metrics of surface-to-air temperature deltas (Ts–a) and scale offsets (errors) are introduced.

Results: Select in situ Ts in direct sunlight are shown to approach or surpass values likely to result in burns to children at touch-scales much finer than Ts resolved by airborne remote sensing. Scale offsets based on neighbourhood and microscale ground observations are 3.8 ◦C and 7.3 ◦C less than the Ts–a at the 1 cm touch-scale, respectively, and 6.6 ◦C and 10.1 ◦C lower than touch-scale playground equipment Ts, respectively. Hence, the coarser scales underestimate high Ts within playgrounds. Both natural (tree) and artificial (shade sail) shade types are associated with significant reductions in Ts.

Conclusions: A scale mismatch exists based on differing methods of urban Ts measurement. The sub-meter touch-scale is the spatial scale at which data must be collected and policies of urban landscape design and health must be executed in order to mitigate high Ts in high-contact environments such as playgrounds. Shade implementation is the most promising mitigation technique to reduce child burns, increase park usability, and mitigate urban heating.

ContributorsVanos, Jennifer K. (Author) / Middel, Ariane (Author) / McKercher, Grant R. (Author) / Kuras, Evan R. (Author) / Ruddell, Benjamin L. (Author)
Created2015-11-10
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In this study, we demonstrate the effectiveness of a cancer type specific FrAmeShifT (FAST) vaccine. A murine breast cancer (mBC) FAST vaccine and a murine pancreatic cancer (mPC) FAST vaccine were tested in the 4T1 breast cancer syngeneic mouse model. The mBC FAST vaccine, both with and without check point

In this study, we demonstrate the effectiveness of a cancer type specific FrAmeShifT (FAST) vaccine. A murine breast cancer (mBC) FAST vaccine and a murine pancreatic cancer (mPC) FAST vaccine were tested in the 4T1 breast cancer syngeneic mouse model. The mBC FAST vaccine, both with and without check point inhibitors (CPI), significantly slowed tumor growth, reduced pulmonary metastasis and increased the cell-mediated immune response. In terms of tumor volumes, the mPC FAST vaccine was comparable to the untreated controls. However, a significant difference in tumor volume did emerge when the mPC vaccine was used with CPI. The collective data indicated that the immune checkpoint blockade therapy was only beneficial with suboptimal neoantigens. More importantly, the FAST vaccine, though requiring notably less resources, performed similarly to the personalized version of the frameshift breast cancer vaccine in the same mouse model. Furthermore, because the frameshift peptide (FSP) array provided a strong rationale for a focused vaccine, the FAST vaccine can theoretically be expanded and translated to any human cancer type. Overall, the FAST vaccine is a promising treatment that would provide the most benefit to patients while eliminating most of the challenges associated with current personal cancer vaccines.
ContributorsMurphy, Sierra Nicole (Author) / Johnston, Stephen (Thesis director) / Peterson, Milene (Committee member) / School of Mathematical and Statistical Sciences (Contributor) / School of Molecular Sciences (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
The goal of this paper is to discuss the most efficient method to achieve early detection in lung cancers by reducing the occurrences of false-positive readings. Imaging techniques (computed tomography screenings) have greater impact than non-imaging techniques in early detection for lung cancer. On the other hand,

The goal of this paper is to discuss the most efficient method to achieve early detection in lung cancers by reducing the occurrences of false-positive readings. Imaging techniques (computed tomography screenings) have greater impact than non-imaging techniques in early detection for lung cancer. On the other hand, positron emission tomography and non-imaging techniques, such as liquid biopsy, are better at distinguishing cancer stages. Therefore, these techniques are not suitable early detection methods for lung cancer. Based on literature reviews, the combination that is most capable of early lung cancer detection incorporate low-dose computed tomography screenings, thin-section computed tomography screenings, and computer-aided diagnosis. Low-dose computed tomography screenings has lower radiation-associated risks compared to the standard-dose computed tomography. This technique can be used as both at the first examination and the follow-up examinations. Thin-section computed tomography screenings can be used as a supplement to check if there is any nodules that have not yet been discovered. Computer-aided diagnosis is an add-on method to make sure the computed tomography screenings images are being correctly labeled. Identifying other contributing factors to the effectiveness of the early lung cancer detection, such as the amount of forced expiratory volume, forced vital capacity, and the presence of emphysema, could also decrease the percentage of false positive outcomes.
ContributorsChuang, Hao-Yun (Author) / Johnston, Stephen (Thesis director) / Peterson, Milene (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
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The objective of this thesis was to determine whether Zika Virus (ZIKV) can be effectively inactivated by Selective Photonic Disinfection (SEPHODIS) and determine whether key proteins involved in the infection process are preserved, making SEPHODIS a possible source for vaccine development. As of January 2018, there have been 3,720 confirmed

The objective of this thesis was to determine whether Zika Virus (ZIKV) can be effectively inactivated by Selective Photonic Disinfection (SEPHODIS) and determine whether key proteins involved in the infection process are preserved, making SEPHODIS a possible source for vaccine development. As of January 2018, there have been 3,720 confirmed cases of Congenital Zika Syndrome in infants, making a Zika Vaccine a high priority (Mitchell, 2018). SEPHODIS is a process that involves prolonged exposure of an object to a pulsing laser which can render it ineffective. Initially, ZIKV was subjected to laser inactivation for 6 hours, then a plaque assay was performed on both laser-treated and control samples. ZIKV was inactivated two-fold? after laser treatment, when compared with control, as indicated by the plaque assay results. Additionally, both samples were submitted to ELISA to evaluate antigenicity with a panel of monoclonal and human sera. As a second control, virus inactivated by formaldehyde (2%) was used. ELISA results showed that antigenicity of some proteins were preserved while others were probably disturbed. However, ELISA results show that ZIKV envelope protein (E-protein), the protein responsible for viral entry into cells, was effectively preserved after laser-treatment, implying that if laser parameters were tweaked to obtain more complete inactivation, then SEPHODIS may be an appropriate source for the development of a vaccine.
ContributorsViafora, Ataiyo Blue (Author) / Johnston, Stephen (Thesis director) / Tsen, Kong-Thon (Committee member) / School of Life Sciences (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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PD-L1 blockade has shown recent success in cancer therapy and cancer vaccine regimens. One approach for anti-PD-L1 antibodies has been their application as adjuvants for cancer vaccines. Given the disadvantages of such antibodies, including long half-life and adverse events related to their use, a novel strategy using synbodies in place

PD-L1 blockade has shown recent success in cancer therapy and cancer vaccine regimens. One approach for anti-PD-L1 antibodies has been their application as adjuvants for cancer vaccines. Given the disadvantages of such antibodies, including long half-life and adverse events related to their use, a novel strategy using synbodies in place of antibodies can be tested. Synbodies offer a variety of advantages, including shorter half-life, smaller size, and cheaper cost. Peptides that could bind PD-L1 were identified via peptide arrays and used to construct synbodies. These synbodies were tested with inhibition ELISA assays, SPR, and pull down assays. Additional flow cytometry analysis was done to determine the binding specificity of the synbodies to PD-L1 and the ability of those synbodies to inhibit the PD-L1/PD-1 interaction. Although analysis of permeabilized cells expressing PD-L1 indicated that the synbodies could successfully bind PD-L1, those results were not replicated in non-permeabilized cells. Further assays suggested that the binding of the synbodies was non-specific. Other tests were done to see if the synbodies could inhibit the PD-1/PD-L1 interaction. This assay did not yield any conclusive results and further experimentation is needed to determine the efficacy of the synbodies in inhibiting this interaction.
ContributorsMujahed, Tala (Author) / Johnston, Stephen (Thesis director) / Blattman, Joseph (Committee member) / Diehnelt, Chris (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
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The devastating 2014 Ebola virus outbreak in Western Africa demonstrated the lack of therapeutic approaches available for the virus. Although monoclonal antibodies (mAb) and other molecules have been developed that bind the virus, no therapeutic has shown the efficacy needed for FDA approval. Here, a library of 50 peptide based

The devastating 2014 Ebola virus outbreak in Western Africa demonstrated the lack of therapeutic approaches available for the virus. Although monoclonal antibodies (mAb) and other molecules have been developed that bind the virus, no therapeutic has shown the efficacy needed for FDA approval. Here, a library of 50 peptide based ligands that bind the glycoprotein of the Zaire Ebola virus (GP) were developed. Using whole virus screening of vesicular stomatitis virus pseudotyped with GP, low affinity peptides were identified for ligand construction. In depth analysis showed that two of the peptide based molecules bound the Zaire GP with <100 nM KD. One of these two ligands was blocked by a known neutralizing mAb, 2G4, and showed cross-reactivity to the Sudan GP. This work presents ligands with promise for therapeutic applications across multiple variants of the Ebola virus.
ContributorsRabinowitz, Joshua Avraam (Author) / Diehnelt, Chris (Thesis director) / Johnston, Stephen (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12
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The growing urban heat island (UHI) phenomenon is having detrimental effects on urban populations and the environment, and therefore, must be addressed. The purpose of this research is to investigate possible strategies that could be utilized to reduce the effects of the urban heat island for the city of Phoenix.

The growing urban heat island (UHI) phenomenon is having detrimental effects on urban populations and the environment, and therefore, must be addressed. The purpose of this research is to investigate possible strategies that could be utilized to reduce the effects of the urban heat island for the city of Phoenix. Current strategies, case studies, and the ENVI-Met modeling software were used to finalize conclusions and suggestions to further progress Phoenix's goals in combating its urban heat island. Results from the studies found that there is much potential in reducing daytime and evening temperatures through improving infrastructure by means of increased vegetation in the forms of green roofs and walls, reducing anthropogenic heat release, improving artificial surface coverage, and implementing lasting policies for further development. Results from the ENVI-met microclimate program shows areas for further research in urban heat island mitigation strategies.
Created2016-12
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Both technological and scientific fields continue to revolutionize in a similar fashion; however, a major difference is that high-tech corporations have found models to continue progressions while still keeping product costs low. The main objective was to identify which, if any, components of certain technological models could be used with

Both technological and scientific fields continue to revolutionize in a similar fashion; however, a major difference is that high-tech corporations have found models to continue progressions while still keeping product costs low. The main objective was to identify which, if any, components of certain technological models could be used with the vaccine and pharmaceutical markets to significantly lower their costs. Smartphones and computers were the two main items investigated while the two main items from the scientific standpoint were vaccines and pharmaceuticals. One concept had the ability to conceivably decrease the costs of vaccines and drugs and that was "market competition". If the United States were able to allow competition within the vaccine and drug companies, it would allow for the product prices to be best affected. It would only take a few small companies to generate generic versions of the drugs and decrease the prices. It would force the larger competition to most likely decrease their prices. Furthermore, the PC companies use a cumulative density function (CDF) to effectively divide their price setting in each product cycle. It was predicted that if this CDF model were applied to the vaccine and drug markets, the prices would no longer have to be extreme. The corporations would be able to set the highest price for the wealthiest consumers and then slowly begin to decrease the costs for the middle and lower class. Unfortunately, the problem within the vaccine and pharmaceutical markets was not the lack of innovation or business models. The problem lied with their liberty to choose product costs due to poor U.S. government regulations.
ContributorsCalderon, Gerardo (Author) / Johnston, Stephen (Thesis director) / Diehnelt, Chris (Committee member) / School of Molecular Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-12