Matching Items (251)
Description
There are two goals for this honors student project: (1) to determine (using an online survey) what college students do and do not know about cooking and preparing foods and (2) to video record short demonstrations of several cooking skills that college students lack the most based on survey responses. Ultimately, this project hopes to help students develop skills they can use in the kitchen to encourage more cooking at home and less eating out, dietary changes that should lead to more healthful meals and a healthier population. Links to cooking videos: https://youtu.be/ufsVYnfoCQM https://youtu.be/aZCIH33ebZ0
ContributorsKarr, Alexandra Rose (Author) / Johnston, Carol (Thesis director) / Jacobs, Mark (Committee member) / School of Nutrition and Health Promotion (Contributor) / W. P. Carey School of Business (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
Many individuals are not following healthy lifestyles as evidenced by the high obesity rates and poor physical activity levels. Many do not know how to eat healthy and exercise effectively, especially given busy schedules and no time to cook or go to the gym. However, people do spend a significant amount of time on their mobile device using social media. This outlet can potentially be used to inspire and teach individuals how to cook healthy simple meals, or find motivation to get off the couch for some exercise. In this study, strategies were identified that would increase the comments, likes, and followers of the investigator's Instagrams, which were designed to motivate followers to live a healthier lifestyle. Initially, 462 posts were assessed retrospectively to determine how the number of likes and comments were influenced by hashtags, category of posts (nutrition, fitness, or inspiration), and/or type of app, PicPlayPost, Instaframe, and the updated Instagram. These results were used in a 1-month prospective study to increase likes and followers to the investigator's Instagrams. In the retrospective study, it was determined that hashtags influenced the number of comments and likes across the categories and that the PicPlayPost app increased the likes in all categories. Hence, for the prospective study, the investigator wrote 1 nutrition and 1 fitness post daily for 28 days using a minimum of 15 hashtags per post. In addition, 3 nutrition posts and 3 fitness posts per week used the PicPlayPost app. This posting strategy increased the number of likes per post by 100%, and the number of followers increased from 369 to 1082 in one month. Interestingly, the nutrition posts received 27% more likes than the fitness posts, and the PicPlayPost app increased comments by 134%. In conclusion, the use of hashtags, PicPlayPost, and Instaframe apps increased the likes and comments on social media. These strategies may increase audience's attention on a certain topic and be used to improve individuals' lifestyles.
ContributorsTomasso, Bernice Grace (Author) / Johnston, Carol (Thesis director) / Dixon, Kathy (Committee member) / Barrett, The Honors College (Contributor) / School of Nutrition and Health Promotion (Contributor)
Created2014-05
Description
Colorectal cancer (CRC) is one of the most highly diagnosed cancers in the United States and accounts for 9.5% of all new cancer cases worldwide. With a 50% five-year prognosis, it is the second highest cancerous cause of death in the U.S. CRC tumors express antigens that are capable of inducing an immune response. The identification of autoantibodies (AAb) against tumor-associated antigens (TAA) may facilitate personalized tumor treatment in the form of targeted immunotherapy. The objective of this study was to observe the AAb expression raised against a 2000 human gene survey in late-stage colorectal cancer using the Nucleic Acid Programmable Protein Arrays (NAPPA). AAbs from serum samples were collected from 80 patients who died within 24 months of their last blood draw and 80 age and gender matched healthy control were profiled using NAPPA. TAA p53, a well-established protein that is one of the most highly mutated across a variety of cancers, was one of the top candidates based on statistical analysis, which, along with its family proteins p63 and p73 (which showed inverse AAb response profiles) warranted further testing via RAPID ELISA. Statistical analysis from these results revealed an inverse differential relationship between p53 and p63, in which p53 seropositivity was higher in patients than in controls, while the opposite was unexpectedly the case for p63. This study involving the AAb immunoprofiling of advanced stage CRC patients is one of the first to shed light on the high-throughput feasibility of immunoproteomic experiments using protein arrays as well as the identification of immunotherapy targets in a more rapid move towards specialized treatment of advanced CRC.
ContributorsSzeto, Emily (Author) / LaBaer, Joshua (Thesis director) / Qiu, Ji (Committee member) / Demirkan, Gokhan (Committee member) / Barrett, The Honors College (Contributor) / T. Denny Sanford School of Social and Family Dynamics (Contributor)
Created2014-12
Description
Height is an essential measure for the evaluation of an adult's health, and especially important for the measure of children's health.4 Lasers have been popularly used in many areas such as construction, engineering, government for defense purposes, and for equestrians. Lasers are depended on for their accuracy, reliability, and ease of use.8, 9 It can be reasoned then that lasers should be a reliable way of measuring height, proving to be accurate and easy to use. Currently, stadiometers are the standard way of measuring height. For the study a laser tool was created to measure the volunteers' heights. Volunteers were recruited from a total of four various public sites in different cities. Participants were categorized into three groups, children (ages 2-12), adolescents (13-18), and adults (19+). A total of 128 participants were measured. Results showed a strong positive correlation between measurements of the stadiometer and the laser (figure 3). While there were limitations to this study, results show that a laser may be a validated tool to measure height accurately
ContributorsGarcia-Turner, Vanessa Marie (Author) / Mayol-Kreiser, Sandra (Thesis director) / Johnston, Carol (Committee member) / Barrett, The Honors College (Contributor) / School of Nutrition and Health Promotion (Contributor) / Department of Psychology (Contributor)
Created2014-05
Description
The focus of this project was to look at alternative treatments for endocrine resistant breast cancer (ERBC), which are breast cancers that have become resistant to hormone therapies such as Tamoxifen or aromatase inhibitors. The first part of this project involves investigating the relationship between histone de-acetylase inhibitor Vorinostat and Tamoxifen in MCF7 G11 cells, Tamoxifen resistant sub-clones, according to the PSOC Time grant. The second part involves targeting the androgen receptor (AR) in MCF7 sub-clones with AR antagonists, Bicalutamide and MDV3100, and investigating the possible usage of AR as a biomarker, due to over-expression of AR in ERBC, in accordance with the Mayo ASU Seed Grant.
The synergistic effects between Vorinostat and Tamoxifen observed through a phase II study on breast cancer patients resistant to hormone therapy may involve more than the modulation of ER-alpha to reverse Tamoxifen resistance in ERBC cells. RT-qPCR of genes expressed in Tamoxifen resistant cells, trefoil factor 1(TFF1) and v-myc avian myelocytomatosis viral oncogene homolog (MYC), were evaluated along with ESR1 and Diablo as a control. MYC was observed to have increased expression in the treated cells, whereas the other genes had a decrease in their expression levels after the cells were treated for 3 days with Vorinostat IC30 of 1 µM. As for targeting the AR, MCF7 Tamoxifen sensitive and resistant cells were not affected by the AR antagonists to determine an IC50. The cell viability for all MCF7 sub-clones only decreased for high concentrations of 5.56 µM - 50 µM in Bicalutamide and 16.67 µM – 50 µM of MDV1300. Furthermore, hormone depletion of MCF7 G11 Tamoxifen resistant sub-clones did not show a great response to DHT stimulation or the AR antagonists. In the RT-qPCR, the MCF7 G11 cells showed an increase in mRNA expression for ER, AR, and PR after 4 hours of treatment with estradiol. As for the DHT treatment, ER, AR, PR, and PSA had a minimal increase in the fold change, but the fold change in AR was less than in the estradiol treatment. The Mayo Clinic will investigate the possible usage of AR as a biomarker through immunohistochemistry.
The synergistic effects between Vorinostat and Tamoxifen observed through a phase II study on breast cancer patients resistant to hormone therapy may involve more than the modulation of ER-alpha to reverse Tamoxifen resistance in ERBC cells. RT-qPCR of genes expressed in Tamoxifen resistant cells, trefoil factor 1(TFF1) and v-myc avian myelocytomatosis viral oncogene homolog (MYC), were evaluated along with ESR1 and Diablo as a control. MYC was observed to have increased expression in the treated cells, whereas the other genes had a decrease in their expression levels after the cells were treated for 3 days with Vorinostat IC30 of 1 µM. As for targeting the AR, MCF7 Tamoxifen sensitive and resistant cells were not affected by the AR antagonists to determine an IC50. The cell viability for all MCF7 sub-clones only decreased for high concentrations of 5.56 µM - 50 µM in Bicalutamide and 16.67 µM – 50 µM of MDV1300. Furthermore, hormone depletion of MCF7 G11 Tamoxifen resistant sub-clones did not show a great response to DHT stimulation or the AR antagonists. In the RT-qPCR, the MCF7 G11 cells showed an increase in mRNA expression for ER, AR, and PR after 4 hours of treatment with estradiol. As for the DHT treatment, ER, AR, PR, and PSA had a minimal increase in the fold change, but the fold change in AR was less than in the estradiol treatment. The Mayo Clinic will investigate the possible usage of AR as a biomarker through immunohistochemistry.
ContributorsVorachitti, Merica (Author) / LaBaer, Joshua (Thesis director) / Anderson, Karen (Committee member) / Gonzalez, Laura (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
Description
Background. Research suggests that fish oil can be used as an intervention to increase clotting times and reduce the risk of cardiovascular diseases.
Objective. The purpose of this study is to examine the effects of fish oil supplementation on blood coagulation parameters in adults with blood type A (BTA) compared to blood type O (BTO) over an eight-week intervention.
Design. The study was a randomized, double-blind dietary intervention using healthy adults with blood types A or O. A total of 18 participants completed the study. Subjects were randomized into two groups: an experimental group (fish oil) made up of 7 BTO and 4 BTA adults, and a control group (coconut oil) made up of 4 BTO and 3 BTA adults. Non-fasting blood was drawn and analyzed for prothrombin time (PT), activated partial thromboplastin time (PTT), and international normalized ratio (INR) at weeks 0 and 8. A food frequency questionnaire was completed at week 0, and anthropometric data collected at weeks 0 and 8.
Results. Baseline PTT results differed significantly between blood types, 28.1±1.4 seconds and 29.7±1.3 seconds for BTA and BTO respectively (p<0.05). Physical activity differed significantly between the experimental and control group at baseline, 53.9±26.8 METS and 86.0±41.9 METS, respectively (p<0.05). In the Fish oil group, prothrombin time increased for BTA vs. BTO, 0.18±0.29 seconds vs -0.11±0.31 seconds respectively (p<0.10indicating a statistical trend). There were no other differences between groups for the other outcome variables.
Conclusion. Fish oil supplementation prolonged clotting time in BTA adults and may be a useful strategy in this population for reducing cardiovascular disease risk. More research is needed to verify and expand these results.
Objective. The purpose of this study is to examine the effects of fish oil supplementation on blood coagulation parameters in adults with blood type A (BTA) compared to blood type O (BTO) over an eight-week intervention.
Design. The study was a randomized, double-blind dietary intervention using healthy adults with blood types A or O. A total of 18 participants completed the study. Subjects were randomized into two groups: an experimental group (fish oil) made up of 7 BTO and 4 BTA adults, and a control group (coconut oil) made up of 4 BTO and 3 BTA adults. Non-fasting blood was drawn and analyzed for prothrombin time (PT), activated partial thromboplastin time (PTT), and international normalized ratio (INR) at weeks 0 and 8. A food frequency questionnaire was completed at week 0, and anthropometric data collected at weeks 0 and 8.
Results. Baseline PTT results differed significantly between blood types, 28.1±1.4 seconds and 29.7±1.3 seconds for BTA and BTO respectively (p<0.05). Physical activity differed significantly between the experimental and control group at baseline, 53.9±26.8 METS and 86.0±41.9 METS, respectively (p<0.05). In the Fish oil group, prothrombin time increased for BTA vs. BTO, 0.18±0.29 seconds vs -0.11±0.31 seconds respectively (p<0.10indicating a statistical trend). There were no other differences between groups for the other outcome variables.
Conclusion. Fish oil supplementation prolonged clotting time in BTA adults and may be a useful strategy in this population for reducing cardiovascular disease risk. More research is needed to verify and expand these results.
ContributorsRyder, Ashley Brooke (Author) / Johnston, Carol (Thesis director) / Lespron, Christy (Committee member) / Barrett, The Honors College (Contributor) / School of Nutrition and Health Promotion (Contributor)
Created2014-05
Description
Early humans adapted to eating cooked food with increased energy density and absorption of macronutrients. However, in modern times many suffer from diseases like obesity and type 2 diabetes which can result from too much energy being absorbed from food. This study measures glucose responses to a high glycemic meal with a side dish of raw or cooked vegetables. There was a slight trend for raw vegetables to have decreased postprandial blood glucose responses when compared to cooked vegetables.
ContributorsWilkins, Christine Marie (Author) / Johnston, Carol (Thesis director) / Jacobs, Mark (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor)
Created2014-05
Description
A coincidence reporter construct, consisting of the p21-promoter and two luciferase genes (Firefly and Renilla), was constructed for the screening of drugs that might inhibit Olig2's tumorigenic role in glioblastoma. The reporter construct was tested using an Olig2 inhibitor, HSP990, as well as short hairpin RNA targeting Olig2. Further confirmatory analysis is needed before the reporter cell line is ready for high-throughput screening at the NIH and lead compound selection.
ContributorsCusimano, Joseph Michael (Author) / LaBaer, Joshua (Thesis director) / Mangone, Marco (Committee member) / Mehta, Shwetal (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2014-05
Description
The pathogenesis of type 1 diabetes (T1D) is still not fully understood in the scientific community. Evidence has shown that viral infections are one of the important environmental factors associated with the disease development. Seven of the top T1D related viruses were selected to study the prevalence of viral humoral response in T1D patients using our innovative protein array platform called Nucleic Acid Programmable Protein Array (NAPPA). In this study, each viral gene was individually captured using various PCR based techniques, cloned into a protein expression vector, and assembled as the first version of T1D viral protein array. Humoral responses of IgG, IgA, and IgM were examined. Although each class of immunoglobulin generated a wide-range of reactivity, responses to various viral proteins from different proteins were observed. In summary, we captured most of the T1D related viral genes, established viral protein expression on the protein array, and displayed the serum response on the viral protein array. The successful progress will help to fulfill the long term goal of testing the viral infection hypothesis in T1D development.
ContributorsDavis, Amy Darlene (Author) / LaBaer, Joshua (Thesis director) / Qiu, Ji (Committee member) / Desi, Paul (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2013-05
Description
Background: The American Heart Association has created an Official 2012 Hands-Only CPR Instructional Video that is approximately one minute in length and has been viewed over 600,000 times on YouTube. Objective: To evaluate the video's effectiveness in teaching adolescents aged 12-17 hands-only CPR. Methodology: The study took place in the Phoenix metropolitan area. Study participants were recruited from high schools, junior high schools and the Tempe Boys & Girls Club. The 100 study participants took a short, four question survey and watched video on either a laptop computer or video projector. Participants were then subjected to a cardiac arrest mock scenario in which they were tested on calling 911, compression rate, compression depth, and hand placement using a Lifeform CPArlene Recording Manikin. In analysis of the data, subjects were separated into four groups: 12-14 year olds (middle school aged) who had previous CPR training (MSG-T), 12-14 year olds with no previous training (MSG-U), 15-17 year olds (high school aged) who had previous training (HSG-T) and 15-17 year olds with no previous training (HSG-U). Results: Every study participant performed hands-only CPR during the mock scenario. Between the two middle school-aged groups, the MSG-U was more likely to call 911 during the mock scenario (P<0.05). There were no significant differences in compression rate and depth between the MSG-U and MSG-T. Between the two high school-aged groups, the HSG-T was more likely to call 911 during the mock scenario (P<0.05). There was no significant difference in compression rate between the HSG-T and HSG-U groups. The HSG-T compressed the chest significantly deeper than the HSG-U group (P<0.05). The HSG-T was the only group to statistically be on par with the AHA recommended 100 compressions/minute (P<0.05). All other groups were significantly below the 100 compressions/minute standard. No groups were statistically on par with the AHA recommended compression depth of two inches. Conclusion: The Official 2012 Hands-Only Instructional Video should not be used as a definitive training tool to teach school-aged adolescents hands-only CPR. This video, as well as other similar training videos, would be useful as introductory tools for children 12-14 years of age or as a refresher for older children who have received previous training.
ContributorsChoppi, Joseph Anthony (Author) / Johnston, Carol (Thesis director) / Hall, Rick (Committee member) / Gross, Toni (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2013-05