While it may be complex, the prevention and treatment of contaminated water is possible. Founded in 2010, 33 Buckets is a registered 501(c)(3) nonprofit organization based out of Tempe, Arizona who partners with vulnerable communities and local partners to provide sustainable access to clean drinking water and WASH (Water and Sanitation for Health) training. Prior to 2018, 33 Buckets had completed drinking water projects in Bangladesh, the Dominican Republic, and Peru. In the summer of 2018, the 33 Buckets team returned to the Cusco region of Peru in an effort to assess more communities in need of clean drinking water infrastructure. In Cusco, 33 Buckets works closely with the Peruvian university, Universidad San Ignacio de Loyola (USIL). The primary purpose of this partnership is to identify communities in the Cusco region with contaminated water sources and a strong interest in improvement of current systems. Throughout this assessment trip, two communities were initially identified as potential partners, Occopata and Mayrasco. The results of bacteria tests showed a presence of Escherichia coli, commonly known as E. coli. When consumed, especially repeatedly, select strains of E. coli will cause severe diarrheal illness. Interviews with community members confirmed that common symptoms of water related disease are prevalent, especially in children. In Occopata and Mayrasco, there is an absence of support for water services from the municipality. Consequently, there is a volunteer-based water advisory board known as Junto Administración de Agua Sanitemeniento (JAAS). JAAS, in most nearby communities, currently utilizes a drip chlorination system in an attempt to disinfect bacterial pathogens from their water source. However, chlorine disinfection requires a precise dosing in order to be effective. In excessive amounts, chlorine will taste and smell of chemicals, likely producing negative feedback from community members. As a result, chlorine levels often are below the necessary level for disinfection. Chlorine tests performed by the 33 Buckets team confirmed that chlorine levels were insufficient to disinfect E. coli.
During the assessment trip, the 33 Buckets team provided a temporary solution to make chlorine disinfection more effective. Following the 2018 assessment trip, 33 Buckets formed a team of student engineers with the primary goal of furthering the technological development of a chlorine disinfection system to be implemented in communities with bacterial infected water sources. This student team was formed through the EPICS (Engineering Projects in Community Service) program at Arizona State University. The purpose of the program is providing a platform for undergraduate engineers to design solutions that create positive impact the greater community. From August of 2018 through April of 2019, the team developed the design for a continuous chlorine disinfection system that automatically tests for residual chlorine levels at multiple points throughout a community. The system is powered entirely from a low-cost solar panel, requiring a minimal amount of sunlight for full functionality. Moving forward, the goal of project development is to create an autonomous feedback loop that will adjust the amount of chlorine exposure to incoming water flows based on the results of the automatic residual chlorine test. The team also hopes to implement automatic data collection for remote monitoring of water quality in addition to onsite test results. The vision of the proposed solution is a network of chlorine disinfection systems around the Cusco region that ultimately will provide access to clean drinking water, indefinitely. This model of user-friendly purification, automatic testing, and data collection can be adjusted and applied to any region around the world experiencing health issues from consumption of contaminated water. A low-cost, scalable, and reliable water disinfection system has the potential to make significant increases in the quality of life for millions of people.
Engineering – The Social Experiment: An Analysis of Engineering Curriculum and Industry Expectations
Science policy and curriculum go hand in. The future engineers are taught hand calculations, lab testing for field work parallels, and methodologies based on the written policies set forth decades ago. Technology today is rapidly changing, and engineering education is struggling to make changes to keep up with these technology advancements. In today’s world, technology drives invention and innovation, whereas some argue it is thought and curiosity. Engineering programs are taking a toll regardless of the point of view. Education is not made to keep up with current societal needs.
This paper a provides an overview of the history of engineering, curriculum standards for engineering programs, an analysis of engineering programs at top universities and large universities alongside student experiences available to engineers. The ideas offered are no means the exact solution; rather policymakers and STEM education stakeholder may find the ideas shared helpful and use them as a catalyst for change.
The purpose of this study was to find an appropriate solution in reducing inflammation around the ankle joint for Rheumatoid Arthritis (RA) patients, so they are able to increase their endurance and improve their overall quality of life. RA patients have to deal with a significant amount of complications that include chronic inflammation, continuous pain in their joints, and overwhelming stress. In addition, it is very common for RA patients to develop severe mental issues that only makes matters worse. As a result, it is imperative that treatments are provided to RA patients to improve their current situation. Three devices from the current market, made for reducing inflammation of the ankle, were chosen for evaluating the effectiveness of each device. It was determined that with 95% confidence that the Gonicc Professional Foot Sleeve was the most effective in reducing inflammation. A prototype was developed based on the feedback of the participants. Further improvements, the prototype will be compared against the Gonicc Professional Foot Sleeve to determine which is the best solution to improve millions of RA patients' lives.
drugs in basic research, biotechnology, diagnostics and therapeutics. However, due to the
cost, labor and time associated with production of antibodies focus has recently changed
towards potential of peptides to act as protein affinity reagents. Affinity peptides are easy
to work with, non-immunogenic, cost effective and amenable to scale up. Even though
researchers have developed several affinity peptides, we are far from compiling library of
peptides that encompasses entire human proteome. My thesis describes high throughput
pipeline that can be used to develop and characterize affinity peptides that bind several
discrete sites on target proteins.
Chapter 2 describes optimization of cell-free protein expression using commercially
available translation systems and well-known leader sequences. Presence of internal
ribosome entry site upstream of coding region allows maximal expression in HeLa cell
lysate whereas translation enhancing elements are best suited for expression in rabbit
reticulocyte lysate and wheat germ extract. Use of optimal vector and cell lysate
combination ensures maximum protein expression of DNA libraries.
Chapter 3 describes mRNA display selection methodology for developing affinity peptides
for target proteins using large diversity DNA libraries. I demonstrate that mild denaturant
is not sufficient to increase selection pressure for up to three rounds of selection and
increasing number of selection rounds increases probability of finding affinity peptide s.
These studies enhance fundamental understanding of mRNA display and pave the way
for future optimizations to accelerate convergence of in vitro selections.
Chapter 4 describes a high throughput double membrane dot blot system to rapidly
screen, identify and characterize affinity peptides obtained from selection output. I used
dot blot to screen potential affinity peptides from large diversity of previously
ii
uncharacterized mRNA display selection output. Further characterization of potential
peptides allowed determination of several high affinity peptides from having Kd range 150-
450 nM. Double membrane dot blot is automation amenable, easy and affordable solution
for analyzing selection output and characterizing peptides without ne ed for much
instrumentation.
Together these projects serve as guideline for evolution of cost effective high throughput
pipeline for identification and characterization of affinity peptides.