Matching Items (2)
Description
There is a relative lack of basic information about early diverging species of the genus Medicago that, for the most part, were formerly considered to be in the genus Trigonella. Species boundaries are not always clear, for example, the most recent treatment of the genus Medicago submerged four previously recognized species into Medicago monantha, a widely distributed species in the Middle East. These species are recognized on the basis of morphological characters such as fruit number, shape, length and areole shape and size, but species identification is still challenging and further clarification of species boundaries is needed. There is also a lack of cytogenetic information. Some of the relatively few published chromosome numbers, e.g. 2n=28, and 44, differ from those of the rest of the genus, which are mostly 2n=16 or multiples thereof, although seven species are 2n=14. As part of a larger study of the genome and chromosome number evolution in the genus Medicago, we obtained genome size data using flow cytometry for 44 accessions of 14 currently recognized early diverging species, with a focus on Medicago monantha. Chromosome numbers were obtained using standard cytological methods. Our chromosome number data confirm a chromosome number of 2n=16 for M. brachycarpa (genome size of 1.33 pg), and M. monspeliaca (1.88 pg), and 2n=28 for M. polyceratia (2.77 pg) and give new numbers for some species; 2n=16 for M. biflora (2.7 pg), and a previously unknown chromosome number for these early diverging species of 2n=14 for Medicago fischeriana (~1.35 pg). Interestingly, our data support the hypothesis that there are at least two entities within M. monantha as currently recognized that differ in chromosome number and genome size; two accessions had chromosome numbers of 2n=26 and 30 with corresponding genome sizes of 2.68 and 2.85 pg and three other accessions had chromosome numbers 2n=36,44, and another 44 with genome sizes of 3.94, 3.89, and 4.04 pg. There are also some significant morphological differences between these two entities, such as fruit length and areole area. These data lead to both further clarification of the relationships of early diverging Medicago and help build a platform for more in-depth research concerning the evolution of chromosome number and genome size within Medicago.
ContributorsSteier, Julia Elizabeth (Author) / Steele, Kelly (Thesis director) / Wojciechowski, Martin (Committee member) / Fehlberg, Shannon (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
Description
In the face of the sixth mass extinction on Earth, with the flowering plant family Cactaceae assessed as the fifth most endangered plant or animal family by the International Union for the Conservation of Nature (IUCN), it is imperative that all available tools be used to understand the biodiversity, habitat suitability, climate change impacts and population viability of cacti. Within the Cactaceae, Mammillaria Haw and the closely related genus Cochemiea (K. Brandegee) Walton of Baja California, Mexico, are species-rich, with 46 regionally endemic taxa, 12 of which have been assessed as threatened or endangered by the IUCN. This study clarifies the evolutionary relationships in the Mammilloid clade, a complex and species-rich clade in tribe Cacteae, and generic circumscription of the genera Mammillaria Haw. and Cochemiea (K. Brandegee) Walton, estimates divergence times, diversification rates and ancestral ranges and explores habitat suitability and the risk of extinction of a representative species within these genera. The r species, Cochemiea halei (K. Brandegee) Walton, a narrowly distributed island endemic, is assessed using species distribution modeling (SDM) and population viability analysis (PVA). SDM in this study includes projections to two climate change scenarios over the next century, using four representative particle concentration pathways, and the PVA uses habitat-specific deterministic and stochastic models. The results of molecular phylogenetic analyses of the Mammilloid cladde restore the genus Mammillaria to monophyly via new combinations in the genus Cochemiea. The taxa in this study are shown to be of recent origin resulting from rapid diversification and radiation. Geological and climatic forces at multiple scales appear to be responsible for the high degree of biodiversity and endemism of these cacti. SDM shows that C. halei is likely to be stranded in its fragmented island habitat, has a facultative adaptation to ultramafic soils, and faces a 21%–53% contraction of its range on the islands under climate change scenarios. PVA suggests that C. halei is at increased risk of extinction in response to slight decreases in fecundity and persistence. In general, the perspectives in this dissertation fill several gaps in our prior knowledge of the evolution, biogeography, and conservation pressures of an important, species-rich group of cacti, occurring in a region of high biodiversity and endemism.
ContributorsBreslin, Peter (Author) / Wojciechowski, Martin F (Thesis advisor) / Albuquerque, Fabio (Committee member) / Fehlberg, Shannon (Committee member) / Majure, Lucas (Committee member) / Rebman, Jon (Committee member) / Arizona State University (Publisher)
Created2020