Matching Items (220)
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Description
The F1Fo ATP synthase is required for energy conversion in almost all living organisms. The F1 complex is a molecular motor that uses ATP hydrolysis to drive rotation of the γ–subunit. It has not been previously possible to resolve the speed and position of the γ–subunit of the F1–ATPase as

The F1Fo ATP synthase is required for energy conversion in almost all living organisms. The F1 complex is a molecular motor that uses ATP hydrolysis to drive rotation of the γ–subunit. It has not been previously possible to resolve the speed and position of the γ–subunit of the F1–ATPase as it rotates during a power stroke. The single molecule experiments presented here measured light scattered from 45X91 nm gold nanorods attached to the γ–subunit that provide an unprecedented 5 μs resolution of rotational position as a function of time. The product of velocity and drag, which were both measured directly, resulted in an average torque of 63±8 pN nm for the Escherichia coli F1-ATPase that was determined to be independent of the load. The rotational velocity had an initial (I) acceleration phase 15° from the end of the catalytic dwell, a slow (S) acceleration phase during ATP binding/ADP release (15°–60°), and a fast (F) acceleration phase (60°–90°) containing an interim deceleration (ID) phase (75°–82°). High ADP concentrations decreased the velocity of the S phase proportional to 'ADP-release' dwells, and the F phase proportional to the free energy derived from the [ADP][Pi]/[ATP] chemical equilibrium. The decreased affinity for ITP increased ITP-binding dwells by 10%, but decreased velocity by 40% during the S phase. This is the first direct evidence that nucleotide binding contributes to F1–ATPase torque. Mutations that affect specific phases of rotation were identified, some in regions of F1 previously considered not to contribute to rotation. Mutations βD372V and γK9I increased the F phase velocity, and γK9I increased the depth of the ID phase. The conversion between S and F phases was specifically affected by γQ269L. While βT273D, βD305E, and αR283Q decreased the velocity of all phases, decreases in velocity due to βD302T, γR268L and γT82A were confined to the I and S phases. The correlations between the structural locations of these mutations and the phases of rotation they affect provide new insight into the molecular basis for F1–ATPase γ-subunit rotation.
ContributorsMartin, James (Author) / Frasch, Wayne D (Thesis advisor) / Chandler, Douglas (Committee member) / Gaxiola, Roberto (Committee member) / Yan, Hao (Committee member) / Arizona State University (Publisher)
Created2012
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Description
Infections caused by the Hepatitis C Virus (HCV) are very common worldwide, affecting up to 3% of the population. Chronic infection of HCV may develop into liver cirrhosis and liver cancer which is among the top five of the most common cancers. Therefore, vaccines against HCV are under intense study

Infections caused by the Hepatitis C Virus (HCV) are very common worldwide, affecting up to 3% of the population. Chronic infection of HCV may develop into liver cirrhosis and liver cancer which is among the top five of the most common cancers. Therefore, vaccines against HCV are under intense study in order to prevent HCV from harming people's health. The envelope protein 2 (E2) of HCV is thought to be a promising vaccine candidate because it can directly bind to a human cell receptor and plays a role in viral entry. However, the E2 protein production in cells is inefficient due to its complicated matured structure. Folding of E2 in the endoplasmic reticulum (ER) is often error-prone, resulting in production of aggregates and misfolded proteins. These incorrect forms of E2 are not functional because they are not able to bind to human cells and stimulate antibody response to inhibit this binding. This study is aimed to overcome the difficulties of HCV E2 production in plant system. Protein folding in the ER requires great assistance from molecular chaperones. Thus, in this study, two molecular chaperones in the ER, calreticulin and calnexin, were transiently overexpressed in plant leaves in order to facilitate E2 folding and production. Both of them showed benefits in increasing the yield of E2 and improving the quality of E2. In addition, poorly folded E2 accumulated in the ER may cause stress in the ER and trigger transcriptional activation of ER molecular chaperones. Therefore, a transcription factor involved in this pathway, named bZIP60, was also overexpressed in plant leaves, aiming at up-regulating a major family of molecular chaperones called BiP to assist protein folding. However, our results showed that BiP mRNA levels were not up-regulated by bZIP60, but they increased in response to E2 expression. The Western blot analysis also showed that overexpression of bZIP60 had a small effect on promoting E2 folding. Overall, this study suggested that increasing the level of specific ER molecular chaperones was an effective way to promote HCV E2 protein production and maturation.
ContributorsHong, Fan (Author) / Mason, Hugh (Thesis advisor) / Gaxiola, Roberto (Committee member) / Chang, Yung (Committee member) / Chen, Qiang (Committee member) / Arizona State University (Publisher)
Created2011
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Description
This study estimates the capitalization effect of golf courses in Maricopa County using the hedonic pricing method. It draws upon a dataset of 574,989 residential transactions from 2000 to 2006 to examine how the aesthetic, non-golf benefits of golf courses capitalize across a gradient of proximity measures. The measures for

This study estimates the capitalization effect of golf courses in Maricopa County using the hedonic pricing method. It draws upon a dataset of 574,989 residential transactions from 2000 to 2006 to examine how the aesthetic, non-golf benefits of golf courses capitalize across a gradient of proximity measures. The measures for amenity value extend beyond home adjacency and include considerations for homes within a range of discrete walkability buffers of golf courses. The models also distinguish between public and private golf courses as a proxy for the level of golf course access perceived by non-golfers. Unobserved spatial characteristics of the neighborhoods around golf courses are controlled for by increasing the extent of spatial fixed effects from city, to census tract, and finally to 2000 meter golf course ‘neighborhoods.’ The estimation results support two primary conclusions. First, golf course proximity is found to be highly valued for adjacent homes and homes up to 50 meters way from a course, still evident but minimal between 50 and 150 meters, and insignificant at all other distance ranges. Second, private golf courses do not command a higher proximity premia compared to public courses with the exception of homes within 25 to 50 meters of a course, indicating that the non-golf benefits of courses capitalize similarly, regardless of course type. The results of this study motivate further investigation into golf course features that signal access or add value to homes in the range of capitalization, particularly for near-adjacent homes between 50 and 150 meters thought previously not to capitalize.
ContributorsJoiner, Emily (Author) / Abbott, Joshua (Thesis director) / Smith, Kerry (Committee member) / Economics Program in CLAS (Contributor) / School of Sustainability (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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This paper will focus on the changes in China's OFDI while also explaining its growth. However, another primary focus will be comparing the relationships between China, Hong Kong, and Africa. This paper will show the correlating changes between the three regions and explain the distribution of China's investments. One argument

This paper will focus on the changes in China's OFDI while also explaining its growth. However, another primary focus will be comparing the relationships between China, Hong Kong, and Africa. This paper will show the correlating changes between the three regions and explain the distribution of China's investments. One argument is that Hong Kong may play a large role in facilitating Chinese investment into Africa, which if not disaggregated, could lead to inaccurate numbers of China's FDI into Africa. The purpose of this paper is to investigate the importance of China's relationship with Hong Kong and Africa. In 2012, Garth Shelton argued that Hong Kong was an important gateway in South Africa's trade with China. Since then, many others have made similar claims in support of Hong Kong's bigger role. However, due to the difficulty of finding specific data for each region, these analyses are incomplete and fail to clearly substantiate their theory. I will try to find a correlation by gathering my own data, tables, and through different interviews.
ContributorsSon, James (Author) / Simonson, Mark (Thesis director) / Iheduru, Okechukwu (Committee member) / Economics Program in CLAS (Contributor) / Department of Finance (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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Pandora is a play exploring our relationship with gendered technology through the lens of artificial intelligence. Can women be subjective under patriarchy? Do robots who look like women have subjectivity? Hoping to create a better version of ourselves, The Engineer must navigate the loss of her creation, and Pandora must

Pandora is a play exploring our relationship with gendered technology through the lens of artificial intelligence. Can women be subjective under patriarchy? Do robots who look like women have subjectivity? Hoping to create a better version of ourselves, The Engineer must navigate the loss of her creation, and Pandora must navigate their new world. The original premiere run was March 27-28, 2018, original cast: Caitlin Andelora, Rikki Tremblay, and Michael Tristano Jr.
ContributorsToye, Abigail Elizabeth (Author) / Linde, Jennifer (Thesis director) / Abele, Kelsey (Committee member) / Department of Information Systems (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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Sagebrush Coffee is a small business in Chandler, Arizona that purchases green beans, roasts them in small batches for quality, and ships fresh, gourmet roasted coffee beans across the nation. Deciding which coffee beans to buy and roast is one of the most crucial business decisions Sagebrush and other gourmet

Sagebrush Coffee is a small business in Chandler, Arizona that purchases green beans, roasts them in small batches for quality, and ships fresh, gourmet roasted coffee beans across the nation. Deciding which coffee beans to buy and roast is one of the most crucial business decisions Sagebrush and other gourmet coffee roasters face. Further complicating this decision is the fact that coffee is a crop, and like all crops, has a specific growing season and the exact same product cannot usually be ordered from year to year, even if it proves to be successful. The goal of this research is to use data analytics and visualization to help Sagebrush make better purchasing decisions by identifying consumer purchasing trends and providing a recommendation for their portfolio mix. In the end, I found that Latin American coffees are popular with both returning and first-time customers, but a specific country of origin does not appear to be associated with the top coffee producing countries. Additionally, December is a critical month for Sagebrush and Sagebrush should make sure to target the states with the most sales: California, Pennsylvania, and New York. Arizona has growth potential as it is not one of the top three locations, despite the presence of a physical store. Also included in the following report is a portfolio recommendation suggesting how many of each product based on region, processing type, and roast level to carry in inventory.
ContributorsBlue, Jessica Morgan (Author) / Kellso, James (Thesis director) / Davila, Eddie (Committee member) / Department of Information Systems (Contributor) / Economics Program in CLAS (Contributor) / Department of Supply Chain Management (Contributor) / Morrison School of Agribusiness (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
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This thesis takes the form of a market research report with the goal of analyzing the implications of the United Kingdom (UK) leaving the European Union (EU) (known as “Brexit”) on London’s office commercial real estate market. The ultimate goal of this report is to make a prediction, firmly grounded

This thesis takes the form of a market research report with the goal of analyzing the implications of the United Kingdom (UK) leaving the European Union (EU) (known as “Brexit”) on London’s office commercial real estate market. The ultimate goal of this report is to make a prediction, firmly grounded in quantitative and qualitative research conducted over the past several months, as to the direction of London’s commercial real estate market going forward (post-Brexit). Within the commercial real estate sector, this paper narrows its focus to the office segment of the London market.

Understanding the political landscape is crucial to formulating a reasonable prediction as to the future of the London market. Aside from research reports and articles, our main insights into the political direction of Brexit come from our recordings from meetings in March of 2017 with two high-ranking members of Parliament and one member of the House of Lords—all of whom are members of the Tory Party (the meetings being held under the condition of anonymity). The below analysis will be followed by a discussion of the economics of Brexit, primarily focusing on the economic risks and uncertainties which have emerged after the vote, and which currently exist today. Such risks include the UK losing its financial passporting rights, weakening GDP and currency value, the potential for a reduction in foreign direct investment (FDI), and the potential loss of the service sector in the city of London due to not being able to access the European Single Market.

The report will shift focus to analyzing three competing viewpoints of the direction of the London market based on recordings from interviews of stakeholders in the London real estate market. One being an executive of one of the largest REITs in the UK, another being the Global Head of Real Estate at a top asset management firm, and another being a director at a large property consulting firm. The report includes these differing “sub-theses” in order to try to make sense of the vast market uncertainties post-Brexit as well as to contrast their viewpoints with where the market is currently and with the report’s investment recommendation.

The remainder of the report will consist of the methods used for analyzing market trends including how the data was modeled in order to make the investment recommendation. The report will analyze real estate and market metrics pre-Brexit, immediately after the vote, post-Brexit, and will conclude with future projections encapsulating the investment recommendation.
ContributorsHorn, Jonathan (Co-author) / Sidi, Adam (Co-author) / Bonadurer, Werner (Thesis director) / McDaniel, Cara (Committee member) / Department of Finance (Contributor) / School of Politics and Global Studies (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2017-12
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This paper explores the contested relationships between nature, culture, and gender. In order to analyze these relationships, we look specifically at outdoor recreation. Furthermore, we employ poststructuralist feminist theory in order to produce three frameworks; the first of which is titled Mother Nature’s Promiscuous Past. Rooted in Old World and

This paper explores the contested relationships between nature, culture, and gender. In order to analyze these relationships, we look specifically at outdoor recreation. Furthermore, we employ poststructuralist feminist theory in order to produce three frameworks; the first of which is titled Mother Nature’s Promiscuous Past. Rooted in Old World and colonial values, this framework illustrates the flawed feminization of nature by masculinity, and its subsequent extortion of anything related to femininity — including women and nature itself. This belief barred women from nature, resulting in a lack of access for women to outdoor recreation.
Our second framework, titled The Pleasurable Potential of Outdoor Recreation, cites second-wave feminism as a catalyst for women’s participation in wilderness exploration and outdoor recreation. The work of radical feminists and the women’s liberation movement in 1960s and 1970s empowered women at home, in the workplace, and eventually, in the outdoors; women reclaimed their wilderness, yet they continued to employ Framework One’s feminization of nature. Ecofeminsim brought together nature and women, seeking to bring justice to two groups wronged by the same entity: masculinity. In this context, outdoor recreation is empowering for women.
Despite the potential of Framework Two to reinscribe and better the experiences of women in outdoor recreation, we argue that both Frameworks One and Two perpetuate the gender binary and the nature/culture binary, because they are based upon the notion that women are in fact fundamentally different and separate from men, the notion that nature is an entity separate from culture, or human society, as well as the notion that nature is in fact a feminine entity.
Our third framework, Deer Pay No Mind to Your Genitals, engages poststructuralism, asserting that outdoor recreation and activities that occur in nature can serve to destabilize and deconstruct notions of the gender binary. However, we argue that care must be exercised during this process as not to perpetuate the problematic nature/culture binary, a phenomenon that is unproductive in terms of both sustainability and gender liberation. Outdoor recreation has been used by many as a tool to deconstruct numerous societal constraints, including the gender binary; this, however, continues to attribute escapist and isolationist qualities toward nature, and therefore perpetuating the nature/culture divide. Ultimately, we argue outdoor recreation can and should be used as a tool deconstruct the gender binary, however needs to account for the fact that if nature is helping to construct elements of culture, then the two cannot be separate.
ContributorsPolick-Kirkpatrick, Kaelyn (Co-author) / Downing, Haley Marie (Co-author) / Dove-Viebahn, Aviva (Thesis director) / Schoon, Michael (Committee member) / School of Sustainability (Contributor) / School of Social Transformation (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Description
Species survive by adapting to what is demanded by their environment. In constant and fluctuating environments, specialist and generalists should be favored, respectively. However, the costs and benefits of adaptation can depend on a variety of factors that alter the intensity of the specialist-generalist trade-off. We examined flight performance to

Species survive by adapting to what is demanded by their environment. In constant and fluctuating environments, specialist and generalists should be favored, respectively. However, the costs and benefits of adaptation can depend on a variety of factors that alter the intensity of the specialist-generalist trade-off. We examined flight performance to determine how well flies that evolved in constant and fluctuating temperatures acclimated to hot and cold temperatures. We predicted that flies would perform best at temperatures most similar to the ones the flies evolved at. Best performance was found when rearing and testing temperatures aligned with the temperature at which a genotype had evolved, with the generalist sharing the best and worst performance combination with the constant thermally evolved flies. Interestingly, evolved and reared temperatures had equal impact on flight performance. It was also observed that rearing at 25°C resulted in flies with the best fitness. These results contribute to the specialist-generalist theory and the idea that long term cold development is restricting in terms of range for thermal performance.
ContributorsLe Vinh Thuy, Jacqueline (Author) / Angilletta, Michael (Thesis director) / VandenBrooks, John (Committee member) / Czarnoleski, Marcin (Committee member) / School of Molecular Sciences (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05
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Despite regional peace agreements, billions of dollars in aid, and the United Nations’ largest peacekeeping mission in the world, conflict in the eastern Democratic Republic of the Congo persists. This paper explores criticisms made by political scientist Séverine Autesserre, who argues that three simplistic narratives revolving around conflict minerals as

Despite regional peace agreements, billions of dollars in aid, and the United Nations’ largest peacekeeping mission in the world, conflict in the eastern Democratic Republic of the Congo persists. This paper explores criticisms made by political scientist Séverine Autesserre, who argues that three simplistic narratives revolving around conflict minerals as a cause, sexual abuse against women and girls as an outcome, and rebuilding central state control as the solution dominate how international interveners view the Congolese conflict. Autesserre further posits that conflict continues because peacebuilding efforts fail to address local dynamics. Using monitoring and evaluation reports of peacebuilding projects in the eastern Congo, primarily from the U.S. Agency for International Development, this paper examines three questions: Do aid agencies have any local peacebuilding projects? If so, do these projects reinforce the dominant narratives? And lastly, do these projects view conflict as a continuum that must be managed through process-oriented objectives, or as a binary phenomenon requiring events-oriented objectives, such as elections? The analysis is based on 10 total reports gathered online, the majority of which are from USAID. Due to a lack of publicly available data and M&E reports on Congo peacebuilding, this collection does not represent a random sample and is not being used to make statistically significant conclusions. Nevertheless, the M&E reports provide a window into how the “rubber meets the road,” so to speak, in terms of how USAID and others view the role of their peacebuilding programs and how to assess programmatic success.

These reports reveal there are certainly some local peacebuilding programs and they do appear to view conflict as a continuum requiring process-oriented goals, such as creating local community mediation organizations. In terms of Autesserre’s three dominant narratives, the results are more mixed. This assortment of seemingly contradictory findings does not mean Autesserre’s arguments are invalid. The USAID Congo Country Strategy document unlocks this apparent contradiction as it explicitly acknowledges Autesserre’s criticisms and appears to move toward finding more nuanced approaches to the conflict. However, at times it still emphasizes the same dominant narratives and state-to-state level approaches. This paper, therefore, concludes that USAID, and potentially others, are in a state of transition between entrenched and evolving narratives. The discord in these evaluations highlights the internal crisis peacebuilders in the Congo are currently facing as they reassess their narratives. In keeping with the self-improving nature of M&E, hopefully these international interveners can move through their narrative transition in an efficient manner, so that they can remain a supportive peacebuilding partner to the Congolese people.
ContributorsSilow, Adam (Author) / Rothenberg, Daniel (Thesis director) / Lake, Milli (Committee member) / School of Politics and Global Studies (Contributor) / Economics Program in CLAS (Contributor) / Barrett, The Honors College (Contributor)
Created2016-05