Located directly south of Pima County, Arizona, there is a culturally rich community known as Santa Cruz County, Arizona. Santa Cruz County is a smaller community being home to an estimated 47,000 people. Santa Cruz County has a rich history given its proximity to the United States-Mexico border. One example of this rich history can be found at the Tumacacori National Historical Park (NHP) with the attractive and unique ruins. The Tumacacori NHP was once an active Spanish mission that oversaw the religious expansion and communal resources. While the Tumacacori NHP no longer operates in this manner, it does serve an important role in providing visitors with a diverse and immersive look into the history of the region and the Tumacacori mission. While the Tumacacori NHP is meant to be a place for all people to visit, most visitors are not from the local community. The reason for this stems from a lack of educational and engagement opportunities between the Tumacacori NHP and the community youth, mainly due to community members being unaware of the park’s existence or the programs that are offered. This project analyzes and uncovers this disconnect as well as the barriers between the Tumacacori NHP and the Santa Cruz County youth through a research study and the use of children’s literature.

Influenza virus A (IVA) poses a serious threat to human health, killing over 25,000 Americans in the 2022 flu season alone. In the past 10 years, vaccine efficacy has varied significantly, ranging from 20-60% each season. Because IVA is subject to high antigenic shift and strain cocirculation, more effective IVA vaccines are needed to reduce the incidence of disease. Herein we report the production of a recombinant immune complex (RIC) vaccine “4xM2e” in Nicotiana benthamiana plants using agroinfiltration for use as a potential universal IVA vaccine candidate. RICs fuse antigen to the C-terminus of an immunoglobulin heavy chain with an epitope tag cognate to the antibody, promoting immune complex formation to increase immunogenicity. IVA matrix protein 2 ectodomain (M2e) is selected to serve as vaccine antigen for its high sequence conservation, as only a small number of minor mutations have occurred since its discovery in 1981 in the human sequence. However, there is some divergence in zoonotic IVA strains, and to account for this, we designed a combination of human consensus, swine, and avian M2e variants, 4xM2e. This was fused to the C terminus of the RIC platform to improve M2e immunogenicity and IVA strain coverage. The 4xM2e RIC was produced in N. benthamiana and verified with SDS-PAGE and Western blot assays, along with an analysis of complex formation and the potential for complement activation via complement C1q ELISA. With this work, we demonstrate the potential of RICs and plant-expression systems to generate universal IVA vaccine candidates.

As fan demographics of major professional sports continue to increase in age, research on youth fandom continues to be a topic of interest for researchers. There are relatively few studies in the space that adequately address the issue of an aging population of fans and highlight the importance of growing fandom starting at a young age. There have been previous studies that have looked at sports fandom and age demographics on a broader scale. This thesis aims to add on to that research by focusing more closely on younger fans and their experience with baseball prior to their college years. The data presented in this thesis was conducted through a predominately qualitative online questionnaire. This research is primarily focused on investigating three topics of interest within the consumer behavior realm of the sports industry. First, to provide a baseline understanding of what factors in childhood potentially impact a person’s likelihood of fandom as they age. Second, to look into promotions and activations that could potentially influence a consumer's behaviors and likelihood of attendance at a Major League game. Third, to provide a comprehensive look at whether or not the understanding of baseball impacts if a person is a fan of the sport. With the data collected from this research, the MLB and individual professional baseball teams can look into ways to capitalize on the findings of the research to promote youth fan engagement. Furthermore, this research identifies a significant need for the league and/or teams to invest in youth fandom and fandom enhancement opportunities.

Since 800 CE— long before the creation of the National Park Service (NPS) in 1905 — the Havasupai Tribe has depended on the natural resources and diverse ecosystem of the Grand Canyon. A deeper examination of how the national park came to fruition however demonstrates an often negated and harrowing history of land theft, forced assimilation via reservation systems, and genocide. Political ecology is a way of considering how power dynamics, resource management, and society intersect. In the United States, this approach exposes the ways that anti-Indigenous legislation originating in the Department of the Interior has functioned both as a driver of ecosystem degradation and structural barricades to Native American self-determination. In order to achieve a just and sustainable future for all, it is necessary to dismantle hegemonic discourses regarding Indigenous communities.