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- Creators: School of Life Sciences
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Description
Laboratory animals represent an invaluable, yet controversial, resource in the field of biomedical research. Animal research has been behind many influential discoveries in the field of emerging therapeutics. They provide the link between the theory of the lab bench and the functional application of medicine to influence human health. The use of animals in research is a consideration which must be heavily weighed, and the implementation must be carried out at a very high standard in order to retain research integrity and responsibility. We are in the process of conducting an experiment using laboratory mice to demonstrate cancer treatment using vaccinia (VACV) mutants as a possible oncolytic therapy for certain strains of melanoma. VACV is a double-stranded DNA poxvirus with a large and easily altered genome. This virus contains many genes dedicated to immune evasion, but has shown sensitivity to cell death by necroptosis in mouse studies (5). We have identified the absence of the kinase RIP3 which is vital in the necroptosis pathway as a potential target for oncolytic therapy using VACV mutants in specific strains of melanoma. Multiple groups of SCID Beige mice were inoculated with different melanoma cell lines and observed for tumor growth. Upon reaching 1 cm3 in volume, tumors were injected with either VACV- Δ83N, VACV- Δ54N, or PBS, and observed for regression. It was hypothesized that melanoma tumors that are RIP3-/- such as the MDA5 cell line will show regression, but melanoma tumors that are RIP3-positive and capable of necroptosis, such as the 2427 cell line, will resist viral replication and continue to proliferate. Our results so far tentatively support this hypothesis, but the data collection is ongoing. Strict and specific protocols with regard to the ethical and responsible use of mice have been implemented and upheld throughout the experiment. Animals are closely monitored, and if their quality of life becomes too poor to justify their continued use in the experiment, they are humanely euthanized, even at the expense of valuable data. The importance of commitment to a high ethical standard is pervasive throughout our work. Animals represent an invaluable contribution to research, and it is important to maintain high standards and transparency with regard to their use. Education and engagement in critical discussions about the use and care of animals in the laboratory contribute to the overall merit and legitimacy of biomedical research in the public and professional eye as a whole, and give legitimacy to the continued use of animals as models to advance science and health.
ContributorsBergamaschi, Julia (Author) / Kibler, Karen (Thesis director) / Jacobs, Bertram (Committee member) / School of Human Evolution and Social Change (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
The major goal of this large project is to develop a Recognition Tunneling Nanopore (RTP) device that will be used for determining the structure of glycosaminoglycans (GAGs). The RTP device is composed of a recognition tunneling junction that is embedded in a nanopore. In order to translocate the GAG molecule through the nanopore, researchers have designed a scheme in which the GAG molecule of interest will be attached to the 5’ end of a DNA primer (figure 1) and the DNA primer will be extended by a biotinylated Φ29 DNA polymerase that is anchored in the nanoslit using streptavidin. This research project specifically is part of a larger project with the main goal of comparing the activity of the wild-type Φ29 DNA polymerase which I have expressed and purified with the mutated Φ29 DNA polymerase devoid of 3’ - 5’ exonuclease activity which was made by Dr. Deng.
ContributorsDadkhah Tirani, Farbod (Author) / Wang, Xu (Thesis director) / Zhang, Peiming (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-05
Description
Individuals with Down Syndrome (DS) are subject to a spectrum of behavioral, cognitive and physical impairments. This population is more predisposed to comorbidity and typically has an increased risk of inactivity resulting in a lower level of fitness. Previous studies on physical activity have shown that routine exercise has similar health benefits for those with DS as those individuals without a disability and in turn progresses their balance ability. Due to limited exercise program opportunities and studies that intentionally investigate the benefits of specific modes of exercise on the DS population, a community-based Exercise Program for Adults with DS (ExDS) was created with the goal of improving their physical and mental health and measuring changes in their balance capabilities throughout the program. ExDS partnered with Arizona State University (ASU) students to create biweekly customized workouts, that followed exercise prescription guidelines, consisting of an aerobic warm-up, main aerobic exercise bout, resistance training, balance training, and stretching for each participant with DS. Participant dynamic and static balance ability was measured using the Berg Balance Scale (BBS) during program pre- and post-assessments. The BBS composite score did not change and no significant improvement was seen in the p-values for each line item of the BBS from pre- to post-testing. For follow-up analyses, the participants with low treatment fidelity were removed. Follow-up analyses showed significant increases in BBS composite score and line item 13 from pre- to post-testing. Treatment fidelity was a limitation in this study and future studies should aim to increase fidelity and consistency of tester for pre- and post-testing. In conclusion, holistic exercise programming for adults with DS appears to benefit balance as long as treatment fidelity is high. It is unclear which mode of exercise had the greatest impact on changes in balance.
ContributorsShikles, Ann Kelly (Author) / Holzapfel, Simon (Thesis director) / Ringenbach, Shannon (Committee member) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
The purpose of this project was to investigate the hypothesis that adults with dyslexia tend to have lower accuracies in and take longer to process tasks involving the serial order of letters, compared to age and gender-matched controls. In Experiment 1, participants evaluated word pairs for differences. Half of the word pairs that they evaluated were the same, whereas the remaining word pairs differed along specific parameters such as sequential rearrangements ("left" vs "felt"), left/right reversals ("cob" vs "cod"), up/down reversals ("best" vs "pest"), homophones ("grown" vs "groan"), visual letter similarities ("tight" vs "fight"), and generic substitutions ("moan" vs "loan"). The response times and accuracies of both groups were recorded. In Experiment 2, the participants spelled single words to dictation using the spelling subtest from the Wechsler Individual Achievement Test\u2014II. Spelling errors were evaluated for errors such as sequential rearrangements, left/right reversals, homophones, substitutions, orthographic violations, omissions, and insertions. An example of a spelling error is the word "excitement" misspelled as "excietment", which involves a sequential rearrangement error. Another example is the word "apparently" misspelled as "aparently,", which involves an error of omission. Error frequencies within these error types for both groups were recorded. Experiment 3 evaluated whether left/right reversal errors during the letter-naming Rapid Automatized Naming and Rapid Alternating Stimulus (RAN/RAS) task were associated with left/right errors during word pair comparison and spelling and whether these visual reversal errors were also associated with errors of serial order. The group with dyslexia was split into two groups: group 1 included participants who did not make any left/right reversals during the RAN/RAS task and group 2 included participants who did make left/right reversals during the RAN/RAS task. The accuracies and reaction times of these three groups during the comparison and spelling assessments were recorded. The results of experiment 1 revealed that that adults with dyslexia had a significantly higher reaction time and lower accuracy during the sequential rearrangement and left/right reversal conditions. Experiment 2 demonstrated that the group with dyslexia made significantly more spelling errors during the homophone and omission conditions. The results of Experiment 3 showed associations between the sequential rearrangement and left/right conditions in both the word pair comparison and spelling task for participants with dyslexia who made left/right reversals during the RAN/RAS task. Overall, the participants with dyslexia who made left/right reversals during the RAN/RAS task seemed to have greater difficulty understanding the orientation of letters that occur on a horizontal plane, since this underlying pattern of errors was also seen throughout the spelling and word comparison tasks. These results show that left/right reversals and errors of serial order are evident in some, but not all adults with dyslexia. These errors may also characterize a distinct subtype of dyslexia. Further, errors of left/right reversal and serial order appear to be associated, so left/right reversals may represent a special form of serial order error that involves a change in the order of visual processing in the horizontal but not vertical axis of letter orientation.
ContributorsAlbert, Andria (Author) / Peter, Beate (Thesis director) / Gray, Shelley (Committee member) / School of International Letters and Cultures (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2018-12
Description
Today’s electric vehicle market is growing at high rates but unaware consumers present a risk to the future success of electric vehicles (EVs). Over the coming years electric vehicles will take over the automotive industry. Consumers will need to become more educated in order to keep up with the rise and adoption of electric vehicles. Automotive companies will play a large part in this future of education for electric vehicles.
This paper uses various studies and my own personal study from my summer internship with Ford Motor Company to determine the biggest consumer fears, challenging them and how automotive companies can combat them. In my opinion, the biggest consumer fears for electric vehicles are range anxiety, lack of a charging network, high initial purchase prices, battery replacement costs and lack of appealing options. Consumers who are not interested in EVs, are interested or just looking have fears that can come from opinions or others opinions. These fears can easily change through factual points.
I have included numerous studies that combat these fears and show the truth of each category. In each category I have used studies that point out reasons why these fears are nothing to worry about. Consumers need to learn these fears can be easily changed and that is what this section aims to do.
Automotive companies will have to play a large part in this educational piece. My paper outlines what they need to do in order to succeed in the future. A major part will be dealer engagement. Dealerships represent the face of the company and if they are unwilling or cannot sell EVs, then electric vehicles will not succeed.
Finally, I provide my personal thoughts on the future of the electric vehicle market and what is holding high adoption rates of EVs back.
This paper uses various studies and my own personal study from my summer internship with Ford Motor Company to determine the biggest consumer fears, challenging them and how automotive companies can combat them. In my opinion, the biggest consumer fears for electric vehicles are range anxiety, lack of a charging network, high initial purchase prices, battery replacement costs and lack of appealing options. Consumers who are not interested in EVs, are interested or just looking have fears that can come from opinions or others opinions. These fears can easily change through factual points.
I have included numerous studies that combat these fears and show the truth of each category. In each category I have used studies that point out reasons why these fears are nothing to worry about. Consumers need to learn these fears can be easily changed and that is what this section aims to do.
Automotive companies will have to play a large part in this educational piece. My paper outlines what they need to do in order to succeed in the future. A major part will be dealer engagement. Dealerships represent the face of the company and if they are unwilling or cannot sell EVs, then electric vehicles will not succeed.
Finally, I provide my personal thoughts on the future of the electric vehicle market and what is holding high adoption rates of EVs back.
ContributorsTisch, Zachary Daniel (Author) / Sully de Luque, Mary (Thesis director) / Tuzzolino, Frank (Committee member) / Thunderbird School of Global Management (Contributor) / Department of Marketing (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Background: This paper details the development of screening and education tools for finding Social Determinants of Health (SDH) in Maricopa County’s vulnerable population. SDH is known as structural or physical differences that hinder everyone to have an equal opportunity for improved health, and one of the steps to overcome the differences is through understanding what SDHs are prevalent in the targeted community, so that the community can develop the needed resources. SDH screening process was developed in collaboration with Student Health Outreach for Wellness (SHOW), a student-run organization whose aim is to provide healthcare for underserved populations. SHOW has a unique multi-disciplinary approach of treating each of their patients, and this screening is planned to be implemented during the treatment.
Methods: A literature review was conducted and SDH screening tool were adapted from known SDH screeners, such as Health Leads, PRAPARE, and CLEAR, to fit to the general population that SHOW serves. Training was also developed to educate the student volunteers who will be conducting the SDH screening and included necessary education about the population and the importance of SDH in general. Training materials include a practice scenario, a resources guide, SMART goal sheet, an introduction to the resource guide, and a process flowchart.
Outcomes: This project consists of different versions of screening tools, a training presentation, activities, handouts, and implementation ideas required to succeed in accurate SDH determination.
Methods: A literature review was conducted and SDH screening tool were adapted from known SDH screeners, such as Health Leads, PRAPARE, and CLEAR, to fit to the general population that SHOW serves. Training was also developed to educate the student volunteers who will be conducting the SDH screening and included necessary education about the population and the importance of SDH in general. Training materials include a practice scenario, a resources guide, SMART goal sheet, an introduction to the resource guide, and a process flowchart.
Outcomes: This project consists of different versions of screening tools, a training presentation, activities, handouts, and implementation ideas required to succeed in accurate SDH determination.
ContributorsChung, Min Kyung Rebecca (Author) / Harrell, Liz (Thesis director) / Essary, Alison (Committee member) / Department of Psychology (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Cancer is a disease in which abnormal cells divide uncontrollably and destroy body tissue, and currently plagues today’s world. Carcinomas are cancers derived from epithelial cells and include breast and prostate cancer. Breast cancer is a type of carcinoma that forms in breast tissue cells. The tumor cells can be further categorized after testing the cells for the presence of certain molecules. Hormone receptor positive breast cancer includes the tumor cells with receptors that respond to the steroid hormones, estrogen and progesterone, or the peptide hormone, HER2. These forms of cancer respond well to chemotherapy and endocrine therapy. On the other hand, triple negative breast cancer (TNBC) is characterized by the lack of hormone receptor expression and tends to have a worse prognosis in women. Prostate cancer forms in the cells of the prostate gland and has been attributed to mutations in androgen receptor ligand specificity. In a subset of triple negative breast cancer, genetic expression profiling has found a luminal androgen receptor that is dependent on androgen signaling. TNBC has also been found to respond well to enzalutamide, a an androgen receptor inhibitor. As the gene of the androgen receptor, AR, is located on the X chromosome and expressed in a variety of tissues, the responsiveness of TNBC to androgen receptor inhibition could be due to the differential usage of isoforms - different gene mRNA transcripts that produce different proteins. Thus, this study analyzed differential gene expression and differential isoform usage between TNBC cancers – that do and do not express the androgen receptor – and prostate cancer in order to better understand the underlying mechanism behind the effectiveness of androgen receptor inhibition in TNBC. Through the analysis of differential gene expression between the TNBC AR+ and AR- conditions, it was found that seven genes are significantly differentially expressed between the two types of tissues. Genes of significance are AR and EN1, which was found to be a potential prognostic marker in a subtype of TNBC. While some genes are differentially expressed between the TNBC AR+ and AR- tissues, the differences in isoform expression between the two tissues do not reflect the difference in gene expression. We discovered 11 genes that exhibited significant isoform switching between AR+ and AR- TNBC and have been found to contribute to cancer characteristics. The genes CLIC1 and RGS5 have been found to help the rapid, uncontrolled growth of cancer cells. HSD11B2, IRAK1, and COL1Al have been found to contribute to general cancer characteristics and metastasis in breast cancer. PSMA7 has been found to play a role in androgen receptor activation. Finally, SIDT1 and GLYATL1 are both associated with breast and prostate cancers. Overall, through the analysis of differential isoform usage between AR+ and AR- samples, we uncovered differences that were not detected by a gene level differential expression analysis. Thus, future work will focus on analyzing differential gene and isoform expression across all types of breast cancer and prostate cancer to better understand the responsiveness of TNBC to androgen receptor inhibition.
ContributorsDeshpande, Anagha J (Author) / Wilson-Sayres, Melissa (Thesis director) / Buetow, Kenneth (Committee member) / Natri, Heini (Committee member) / School of Human Evolution & Social Change (Contributor) / School of Life Sciences (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Childbirth, an essential stage of human life, has been carried out and treated differently in numerous ways throughout time. Although the overall method of birth is biologically the same, women and medical professionals in the United States in particular have changed how they view and manage childbirth over the past 70 years. Some of said changes are extensive and occurred more rapidly than one might typically expect for such a delicate and important stage of a woman‘s, and infant‘s, life. As consumerism, capitalism, and the courts have changed America‘s lifestyles, politics, and society, so too have they drastically affected the way we are conditioned to approach childbirth. More importantly, as society changes over time, the medical field and
methods of specialists also change, and although the benefits of these changes are challenged by some individuals, these procedures and recommendations from professionals inevitably affect us all. Methods and procedures of modern, medicalized childbirth, and even the significance placed on the event, are products of historical and cultural factors influenced by scientific and social trends. However, there exists a small and steadily growing number of women and families who choose to have their birth take place outside of the present societal norm, and consequently outside of hospitals. This group‘s existence and growth has been attributed to several factors, including changes in societal values, differentiation between different financial classes, and the
medicalization of childbirth. Although statistically a small percentage of the majority, these women who choose to give birth outside of a hospital exist amidst an immense ongoing controversy between gynecologists, physicians, mothers, and midwives regarding what options should be available when childbirth is undertaken in the United States.
methods of specialists also change, and although the benefits of these changes are challenged by some individuals, these procedures and recommendations from professionals inevitably affect us all. Methods and procedures of modern, medicalized childbirth, and even the significance placed on the event, are products of historical and cultural factors influenced by scientific and social trends. However, there exists a small and steadily growing number of women and families who choose to have their birth take place outside of the present societal norm, and consequently outside of hospitals. This group‘s existence and growth has been attributed to several factors, including changes in societal values, differentiation between different financial classes, and the
medicalization of childbirth. Although statistically a small percentage of the majority, these women who choose to give birth outside of a hospital exist amidst an immense ongoing controversy between gynecologists, physicians, mothers, and midwives regarding what options should be available when childbirth is undertaken in the United States.
ContributorsHernandez, Dustin (Author) / Nguyen, Christy (Author) / Koblitz, Ann (Thesis director) / Budolfson, Arthur (Committee member) / Walker, Shell (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / W. P. Carey School of Business (Contributor)
Created2012-12
Description
Patient-physician interactions are the cornerstone of healthcare delivery, with the potential to significantly influence patient outcomes and healthcare efficiency. The quality of patient-physician interactions is pivotal in facilitating efficient communication concerning patient care and treatment. This relationship impacts the patient's adherence to medical advice and trust in healthcare. Considering the diversity of the patient population, there are a multitude of pertinent variables to take into account, including but not limited to English proficiency, race, educational attainment, age, gender, and socioeconomic status. How do patient-physician communication patterns and demographic variables impact patient comprehension, perception of communication efficacy, and healthcare outcomes across diverse cultural, linguistic, and socioeconomic backgrounds? The purpose of this thesis is to comprehensively understand the characteristics underlying effective patient-physician communication and its impact on patient compliance, retention of medical information, and healthcare outcomes. By addressing racial, cultural, linguistic, and socioeconomic disparities in healthcare, the research aims to establish a universal foundation for augmenting patient-centered care. The methodology of this research included an extensive literature review, shadowing of clinical visits, and patient care, along with the distribution of a survey to patients to gain insight into their satisfaction with healthcare. Practical applications include the development of targeted interventions, communication training programs for healthcare providers, and the formulation of policies aimed at improving patient outcomes and healthcare system efficiency. This research paper investigates the impact of physician attitudes on patient understanding and overall health through a comprehensive analysis of survey data collected from 115 individuals attending various clinics. Our findings highlight the significance of quality healthcare delivery in fostering favorable patient experiences, with 63.5% of respondents rating their visits as excellent or very good. Effective communication emerges as a key determinant of patient satisfaction, with 64.4% of respondents rating communication as excellent or very good. However, a notable proportion of respondents (26.9%) rated their overall satisfaction as neutral or dissatisfied, indicating areas for improvement in patient satisfaction related to unaddressed concerns or inadequate communication. Gender-related concerns, reactive versus proactive medicine, mental health, and shared decision-making emerge as overlooked topics in current clinical practice. Our observations underscore the need for a holistic approach that addresses patients' psychosocial and emotional needs alongside medical concerns. Gender-related differences in care delivery are evident, with female patients reporting feeling dismissed or misunderstood by male doctors, particularly regarding issues related to pain or reproductive health. Female physicians are associated with a greater likelihood of receiving preventive counseling and gender-specific screening, highlighting the importance of gender diversity in healthcare. Furthermore, patients express frustration with a reactive approach to medicine, advocating for a higher focus on addressing the underlying causes of health issues rather than merely treating symptoms. Patients also desire more information about natural remedies and holistic treatment options, emphasizing the importance of incorporating these discussions into shared decision-making between doctors and patients. The study underscores the pivotal role of specific provider qualities such as communication, empathy, and attention to patient comfort in patient-centered care delivery. The comprehension and cooperation rating between the doctor and patient was 81.8% excellent and good with a remaining of 18.2% indicating the need for a more interactive visit. Based on the survey, the important qualities of a provider from highest to lowest ranking include: communication (listening and understanding), empathy and kindness, medical knowledge proficiency, and quality listening. Due to the heavy importance on communication, it is imperative for healthcare providers to gain the necessary skills to cater to and address many of the concerns of their patients outside of simple medical knowledge. Given the doctor explanation comprehension rating of 25.2% being somewhat clear and not so clear, it is necessary for providers to develop their patient communication skills to optimize patient satisfaction and compliance. Cultural competency emerges as a critical aspect of providing effective and equitable care to patients from diverse backgrounds. However, there are limitations to the study, including the relatively small sample size and potential response bias. The findings of this study provide valuable insights into the multifaceted nature of the doctor-patient interaction and underscore the importance of effective communication, patient-centered care, and shared decision-making in promoting positive patient outcomes. According to the patient experience survey, talking more in depth about the patient treatment plan and taking the time to display eagerness to help and be active in one’s health will significantly enhance visit satisfaction. In addition to patients' ratings of physician level of empathy and respect consisting of 93% of respondents as exceeded or met expectations, the patient confidence in their health situation out of 10 was 6 and above for 90 percent of the survey respondents. However, with the shared decision making rating, 28.3% of respondents felt being somewhat involved or a little involved in their own care. By addressing patient needs and preferences and fostering collaborative relationships between physicians and patients, healthcare providers can enhance patient satisfaction, adherence to treatment recommendations, and overall health outcomes.
ContributorsAbdul, Zahir (Author) / La Crosse, Amber (Co-author) / Agu, Nnenna (Thesis director) / Markabawi, Bashar (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Department of Psychology (Contributor)
Created2024-05
Description
This study examines the complex relationship between depression and students' experiences in active learning science courses. We uncover the significant impact of depression on cognitive functioning, particularly affecting energy levels, motivation, and self-doubt, especially among women. Highlighting the intersectionality of gender and depression, we stress the need to address disparities and bolster confidence in academic settings.
ContributorsClark, Sarah (Author) / Cooper, Katelyn (Thesis director) / Brownell, Sara (Committee member) / Barrett, The Honors College (Contributor) / School of Life Sciences (Contributor) / Dean, The College of Liberal Arts and Sciences (Contributor)
Created2024-05