Matching Items (330)
Description
Background: Chemistry and particularly enzymology at surfaces is a topic of rapidly growing interest, both in terms of its role in biological systems and its application in biocatalysis. Existing protein immobilization approaches, including noncovalent or covalent attachments to solid supports, have difficulties in controlling protein orientation, reducing nonspecific absorption and preventing protein denaturation. New strategies for enzyme immobilization are needed that allow the precise control over orientation and position and thereby provide optimized activity.
Methodology/Principal Findings: A method is presented for utilizing peptide ligands to immobilize enzymes on surfaces with improved enzyme activity and stability. The appropriate peptide ligands have been rapidly selected from high-density arrays and when desirable, the peptide sequences were further optimized by single-point variant screening to enhance both the affinity and activity of the bound enzyme. For proof of concept, the peptides that bound to β-galactosidase and optimized its activity were covalently attached to surfaces for the purpose of capturing target enzymes. Compared to conventional methods, enzymes immobilized on peptide-modified surfaces exhibited higher specific activity and stability, as well as controlled protein orientation.
Conclusions/Significance: A simple method for immobilizing enzymes through specific interactions with peptides anchored on surfaces has been developed. This approach will be applicable to the immobilization of a wide variety of enzymes on surfaces with optimized orientation, location and performance, and provides a potential mechanism for the patterned self-assembly of multiple enzymes on surfaces.
ContributorsFu, Jinglin (Author) / Reinhold, Jeremy (Author) / Woodbury, Neal (Author) / Biodesign Institute (Contributor)
Created2011-04-08
Description
Incorporating sustainability education in primary healthcare practice is an important step toward promoting sustainability in the US healthcare system. This health strategy is also consistent with a renewed focus of the US healthcare system, mainly prompted by the Affordable Healthcare Act of 2010, toward preventive care and patient wellness. The major challenge, however, is an effective implementation of sustainability education in the healthcare industry. This honors thesis project developed a sustainability education model, in which primary healthcare providers or the physicians educated the patients about sustainability and its connection to public health issues. The main purpose of this thesis project is to analyze the effectiveness of this sustainability education model and to evaluate its impact on the individuals and households in terms of sustainable attitudes and practices. The study was conducted with 26 parents of newborn babies at Estrella Pediatrics PC using a classic randomized control-group pretest-posttest design. The Pre- and Post-Surveys were completed to evaluate change in their knowledge and attitudes toward sustainability practices covered in the sustainability education model. In the research, the relationships between sustainability-related issues and their negative impacts on the health of human beings were established in the sustainability education pamphlet provided to the physicians, which they shared with the patients during the wellness visits. This pamphlet focused on waste management, air pollution, and locally grown food. Moreover, samples of environmentally-friendly diapers were given to the study respondents to complement this education. The study demonstrated positive trends with the intervention protocol, though the level of statistical significance was marginal. More specifically, it was observed that the respondents placed the highest significance on the education provided by the pediatricians. Interestingly, the receipt of the diaper samples by itself did not generate any significant effect. However, the education provided by the physician and the pamphlet coupled with the diaper gave very positive results. In conclusion, physician led sustainability education has significant potential in promoting sustainability in primary healthcare practice, and further inquiry should be pursued.
ContributorsQuintero Cervantes, Laura Beatriz (Author) / Shrestha, Milan (Thesis director) / Boone, Christopher (Committee member) / Thunderbird School of Global Management (Contributor) / W. P. Carey School of Business (Contributor) / School of Sustainability (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
The purpose of this paper was to assist companies involved in international/intercultural business negotiations establish an orderly, informed, prepared, and empowered negotiation team within the corporate structure. The paper introduces and reinforces the importance of a systematic rather than situational approach to intercultural negotiations and assists companies establish the infrastructure, team, team coordination and alignment, preparation for negotiations, and intercultural skills necessary to maximize their success. China was chosen as the hypothetical international opponent because of its importance to the world economy and the value to American companies of establishing a business presence there. It also presented a prime example of the complexity of negotiations that has been so deeply influenced by a 5,000-year-old culture. In order to validate other research materials, the experiences of three expatriates who have lived in China and worked as business consultants there were explored in interviews. Each presented disparate views on the role of culture in successful negotiations, which underscored the importance of preparation to the negotiation process. The result was an outline of how a company can assemble and incorporate a team of ever-improving professionals for any foray into the global marketplace, with specific recommendations for expanding into China. Although the primary focus is on China, the suggestions and principles contained in this paper can be applied to any company of any national origin attempting to enter the global marketplace. By following some or all of its suggestions, companies will be in a better position to participate effectively in international / intercultural negotiations.
ContributorsChen, Yutong (Author) / Goldman, Alan (Thesis director) / Frost, Donald (Committee member) / Thunderbird School of Global Management (Contributor) / W. P. Carey School of Business (Contributor) / Department of Management (Contributor) / Barrett, The Honors College (Contributor)
Created2015-12
Description
Joseph Rotblat (1908-2005) was the only physicist to leave the Manhattan Project for moral reasons before its completion. He would spend the rest of his life advocating for nuclear disarmament. His activities for disarmament resulted in the formation, in 1957, of the Pugwash conferences, which emerged as the leading global forum to advance limits on nuclear weapons during the Cold War. Rotblat's efforts, and the activities of Pugwash, resulted in both being awarded the Nobel Peace Prize in 1995. Rotblat is a central figure in the global history of resistance to the spread of nuclear weapons. He also was an important figure in the emergence, after World War II, of a counter-movement to introduce new social justifications for scientific research and new models for ethics and professionalism among scientists. Rotblat embodies the power of the individual scientist to say "no" and thus, at least individually, put limits of conscience on his or her scientific activity. This paper explores the political and ethical choices scientists make as part of their effort to behave responsibly and to influence the outcomes of their work. By analyzing three phases of Rotblat's life, I demonstrate how he pursued his ideal of beneficial science, or science that appears to benefit humanity. The three phases are: (1) his decision to leave the Manhattan Project in 1944, (2) his role in the creation of Pugwash in 1957 and his role in the rise of the organization into international prominence and (3) his winning the Nobel Peace Prize in 1995. These three phases of Rotblat's life provide a singular window of the history of nuclear weapons and the international movement for scientific responsibility in the 50 years since the bombing of Hiroshima in 1945. While this paper does not provide a complete picture of Rotblat's life and times, I argue that his experiences shed important light on the difficult question of the individual responsibility of scientists.
ContributorsEvans, Alison Dawn (Author) / Zachary, Gregg (Thesis director) / Hurlbut, Ben (Committee member) / Francis, Sybil (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2015-05
Description
The purpose of this study was to determine the ratio of vegetable to fruit incorporated during a fresh vegetable and/or fruit juice diet. Juicing is the process of extracting the liquid part of a plant, fruit, or vegetable. Food can be ground, pressed, and spun to separate the liquid from the pulp. A juice diet involves juicing and consuming a variety of vegetables and fruits. The primary objective of this study was to gather information about the ratio of vegetable to fruit incorporated in freshly made juices during a juice diet. Therefore, the study survey inquired about various topics related to ingredient ratio during a juice diet. The survey data allowed for examination of the relationships between ingredient ratio and certain variables (e.g. gender, age, length of time juicing, juice fast participation, health effects, etc.). The study participants were recruited using online social media. Facebook was the primary method for reaching the online juicing community. A written invitation was distributed in several health related Facebook groups encouraging any person with experience juicing to complete an anonymous survey. This post was also shared via Twitter and various health related websites. The study survey data was used to examine the relationships between ingredient ratio and specific variables. The survey data showed participants had varying levels of experience with juicing. The responses indicated many participants were familiar with juice fasting and many participants completed more than one juice fast. Based on the survey response data, the most common ratio of vegetable to fruit incorporated by the participants during a juice diet was 80% vegetable to 20% fruit. The majority of participants indicated daily consumption of freshly made juice containing 70% -100% vegetables. Based on the survey response data, beginner juicers may be less inclined to incorporate organic produce into their juice diet compared to advanced juicers. The majority of participants reported positive health benefits during a juice diet. Some of the positive health benefits indicated by participants include weight loss, increased energy, and a positive impact on disease symptoms. Some of the negative side effects experienced by participants during a juice diet include frequent urination, headache, and cravings. Cross tabulation calculations between the ratio of ingredients and several variables covered by the study survey demonstrated statistical significance (i.e. length of time juicing, frequency of drinking juice, juice fast participation, number of juice fasts completed, servings of vegetables/fruit in a juice, percent of organic vegetables/fruit used in a juice, perceived positive side effects, and perceived negative side effects). This study provided insight about the average ratio of vegetable to fruit incorporated by participants during a juice diet. When analyzing the data it is important to consider the survey data was self-reported. Therefore, every result and conclusion is based on the individual perceptions of the study participants. In future experimentation, the use of medical tests and blood work would be useful to determine the biological and biochemical effects of drinking raw vegetable and/or fruit juice on the human body.
ContributorsMata, Sara Ann (Author) / Mayol-Kreiser, Sandra (Thesis director) / Shepard, Christina (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Today’s electric vehicle market is growing at high rates but unaware consumers present a risk to the future success of electric vehicles (EVs). Over the coming years electric vehicles will take over the automotive industry. Consumers will need to become more educated in order to keep up with the rise and adoption of electric vehicles. Automotive companies will play a large part in this future of education for electric vehicles.
This paper uses various studies and my own personal study from my summer internship with Ford Motor Company to determine the biggest consumer fears, challenging them and how automotive companies can combat them. In my opinion, the biggest consumer fears for electric vehicles are range anxiety, lack of a charging network, high initial purchase prices, battery replacement costs and lack of appealing options. Consumers who are not interested in EVs, are interested or just looking have fears that can come from opinions or others opinions. These fears can easily change through factual points.
I have included numerous studies that combat these fears and show the truth of each category. In each category I have used studies that point out reasons why these fears are nothing to worry about. Consumers need to learn these fears can be easily changed and that is what this section aims to do.
Automotive companies will have to play a large part in this educational piece. My paper outlines what they need to do in order to succeed in the future. A major part will be dealer engagement. Dealerships represent the face of the company and if they are unwilling or cannot sell EVs, then electric vehicles will not succeed.
Finally, I provide my personal thoughts on the future of the electric vehicle market and what is holding high adoption rates of EVs back.
This paper uses various studies and my own personal study from my summer internship with Ford Motor Company to determine the biggest consumer fears, challenging them and how automotive companies can combat them. In my opinion, the biggest consumer fears for electric vehicles are range anxiety, lack of a charging network, high initial purchase prices, battery replacement costs and lack of appealing options. Consumers who are not interested in EVs, are interested or just looking have fears that can come from opinions or others opinions. These fears can easily change through factual points.
I have included numerous studies that combat these fears and show the truth of each category. In each category I have used studies that point out reasons why these fears are nothing to worry about. Consumers need to learn these fears can be easily changed and that is what this section aims to do.
Automotive companies will have to play a large part in this educational piece. My paper outlines what they need to do in order to succeed in the future. A major part will be dealer engagement. Dealerships represent the face of the company and if they are unwilling or cannot sell EVs, then electric vehicles will not succeed.
Finally, I provide my personal thoughts on the future of the electric vehicle market and what is holding high adoption rates of EVs back.
ContributorsTisch, Zachary Daniel (Author) / Sully de Luque, Mary (Thesis director) / Tuzzolino, Frank (Committee member) / Thunderbird School of Global Management (Contributor) / Department of Marketing (Contributor) / Barrett, The Honors College (Contributor)
Created2019-05
Description
Thunderbird School of Global Management within Arizona State University offers many degree programs, such as a Bachelor of Global Management, Master of Global Management (with over sixteen different concentration options), and more. Thunderbird is also home to additional educational programs, such as Executive Education and the Najafi 100 Million Learners Global Initiative. The skeleton of a program this paper proposes will add to these learning opportunities and pitch a non-credit additional learning program for the BGM/BSIT/MGM target market. Such a program is currently nonexistent and would add considerably to the students; learning experiences.
ContributorsWalsh, Katherine Anna (Author) / Ault, Joshua (Thesis director) / Vazquez Ortiz, Eva (Committee member) / Barrett, The Honors College (Contributor) / School of International Letters and Cultures (Contributor) / Thunderbird School of Global Management (Contributor) / Department of Information Systems (Contributor)
Created2024-05
Description
This duo thesis is an autoethnography of what it means to be Filipino-American in the United States. Being raised in varying backgrounds yet with the similarity of being Filipino, there are aspects of our lives that are simultaneously similar and different. The goal of this project is the exploration of one’s cultural, familial and personal identities and how they intersect with their individuality and sense of belonging. Through this project, we documented our experience as Filipinos in the United States and our travel back to the Philippines and showcased it through videos. It is a means to document our reflections on this project to share with the Filipino community and individuals who share the same sentiments.
ContributorsTamayo, Rianne Daisy (Author) / Onayan, Youla Tricia (Co-author) / O'Flaherty, Katherine (Thesis director) / Fedock, Rachel (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2024-05
Description
This duo thesis is an autoethnography of what it means to be Filipino-American in the United States. Being raised in varying backgrounds yet with the similarity of being Filipino, there are aspects of our lives that are simultaneously similar and different. The goal of this project is the exploration of one’s cultural, familial and personal identities and how they intersect with their individuality and sense of belonging. Through this project, we documented our experience as Filipinos in the United States and our travel back to the Philippines and showcased it through videos. It is a means to document our reflections on this project to share with the Filipino community and individuals who share the same sentiments.
ContributorsOnayan, Youla Tricia (Author) / Tamayo, Rianne Daisy (Co-author) / O'Flaherty, Katherine (Thesis director) / Fedock, Rachel (Committee member) / Barrett, The Honors College (Contributor) / College of Health Solutions (Contributor)
Created2024-05
Description
MyCollegeCooking.com is a student-driven initiative aimed at revolutionizing the way college students approach nutrition and cooking. Understanding the unique challenges faced by students, such as limited space and time constraints, our platform provides accessible tools and inspiration for preparing nutritious meals. Beyond offering recipes, our website includes detailed nutritional information and encourages interaction from users, fostering a dynamic community. Supported by research and feedback from over 100 college students, our focus on simplicity, accessibility, and balance addresses the common concerns of time and money. Through strategic marketing efforts, particularly leveraging social media, we aim to raise awareness and promote healthy cooking habits among college students nationwide. MyCollegeCooking.com isn't just a recipe website; it's a collaborative platform dedicated to enhancing the well-being and success of students through nutritious eating and community engagement.
ContributorsSpreitzer, Nicole (Author) / Rennie, Isabel (Co-author) / Modic, Jill (Co-author) / Vandeest, Maren (Co-author) / Byrne, Jared (Thesis director) / Lee, Peggy (Committee member) / Balvan, Rachel (Committee member) / Barrett, The Honors College (Contributor) / Thunderbird School of Global Management (Contributor) / School of International Letters and Cultures (Contributor)
Created2024-05