Matching Items (324)
Description
Nucleic acids encode the information required to create life, and polymerases are the gatekeepers charged with maintaining the storage and flow of this genetic information. Synthetic biologists utilize this universal property to modify organisms and other systems to create unique traits or improve the function of others. One of the many realms in synthetic biology involves the study of biopolymers that do not exist naturally, which is known as xenobiology. Although life depends on two biopolymers for genetic storage, it may be possible that alternative molecules (xenonucleic acids – XNAs), could be used in their place in either a living or non-living system. However, implementation of an XNA based system requires the development of polymerases that can encode and decode information stored in these artificial polymers. A strategy called directed evolution is used to modify or alter the function of a protein of interest, but identifying mutations that can modify polymerase function is made problematic by their size and overall complexity. To reduce the amount of sequence space that needs to be samples when attempting to identify polymerase variants, we can try to make informed decisions about which amino acid residues may have functional roles in catalysis. An analysis of Family B polymerases has shown that residues which are involved in substrate specificity are often highly conserved both at the sequence and structure level. In order to validate the hypothesis that a strong correlation exists between structural conservation and catalytic activity, we have selected and mutated residues in the 9°N polymerase using a loss of function mutagenesis strategy based on a computational analysis of several homologues from a diverse range of taxa. Improvement of these models will hopefully lead to quicker identification of loci which are ideal engineering targets.
ContributorsHaeberle, Tyler Matthew (Author) / Chaput, John (Thesis director) / Chen, Julian (Committee member) / Larsen, Andrew (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
After researching pediatric cancer experiences, an opportunity emerged creating a less intimidating environment for children undergoing chemotherapy. By means of adding a creative component to their IV pole and disguising machinery, children will be a part of an Imagination Voyage adventure. Creative themes allow for a journey on a pirate ship, or being in a fantasy castle by captivating children in playtime. The design allows for a frightening experience to become a positive one.
ContributorsHerold, Brittany Ann (Author) / Shin, Dosun (Thesis director) / McDermott, Lauren (Committee member) / Barrett, The Honors College (Contributor) / Herberger Institute for Design and the Arts (Contributor) / School of Sustainability (Contributor) / The Design School (Contributor)
Created2015-05
Description
I set out to better understand the issues, perceptions & solutions surrounding drought. The question that compelled my project was "What might be all the ways that we can improve the experience of conserving, reusing & educating on the topic of water." Through the process of design research I developed a system of products that improves the user experiences surrounding water. The result is IOW, an intelligent 3-product system that aims to make your water needs & wants smarter & less wasteful.
ContributorsShappee, Christian Kyle (Author) / Shin, Dosun (Thesis director) / McDermott, Lauren (Committee member) / Barrett, The Honors College (Contributor) / Herberger Institute for Design and the Arts (Contributor) / School of Sustainability (Contributor) / The Design School (Contributor)
Created2015-05
Description
The fashion industry dubs couture as high fashion, yet couture never reaches the finish line when it comes to comfort. Most of the brand name high heels on the market are too painful to wear for long periods of time. For this project, I have developed 3D printed high heels with detachable insoles that will relieve tired feet based on the principle of reflexology. The product integrates traditional flexible insoles with Arduino computing and the result is a functional surface that can ease the pain of the wearer. This paper introduces the product and with it, under-explored opportunities to customize your own high heels at home. Essentially, each consumer will have the ability to personalize and switch out their style without sacrificing comfort. Soon, a consumer will be a designer.
ContributorsNguyen, Nhi N. (Author) / Ingalls, Todd (Thesis director) / Gigantino, Josh (Committee member) / Barrett, The Honors College (Contributor) / Herberger Institute for Design and the Arts (Contributor) / School of Arts, Media and Engineering (Contributor)
Created2015-05
Description
Lung cancer is the leading cause of cancer-related deaths in the US. Low-dose computed tomography (LDCT) scans are speculated to reduce lung cancer mortality. However LDCT scans impose multiple risks including false-negative results, false- positive results, overdiagnosis, and cancer due to repeated exposure to radiation. Immunosignaturing is a new method proposed to screen and detect lung cancer, eliminating the risks associated with LDCT scans. Known and blinded primary blood sera from participants with lung cancer and no cancer were run on peptide microarrays and analyzed. Immunosignatures for each known sample collectively indicated 120 peptides unique to lung cancer and non-cancer participants. These 120 peptides were used to determine the status of the blinded samples. Verification of the results from Vanderbilt is pending.
ContributorsNguyen, Geneva Trieu (Author) / Woodbury, Neal (Thesis director) / Zhao, Zhan-Gong (Committee member) / Stafford, Phillip (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor) / Department of Psychology (Contributor)
Created2015-05
Description
Using DNA nanotechnology a library of structures of various geometries have been built; these structures are modified chemically and/or enzymatically at nanometer precisions. With DNA being chemically very stable, these structures can be functionalized through an abundance of well-established protocols. Additionally, they can be used for various biological and medicinal purposes, such as drug delivery. For in vivo applications, the DNA nanostructures must have a long circulation life in the bloodstream; otherwise, they could be easily excreted shortly after entry. One way of making these nanostructures long lasting in the blood is to cover them with the biocompatible polymer, polyethylene glycol (PEG). Adding DNA to PEG before forming structures has been found to interfere in the hybridization of the DNA in the structure, resulting in formation of deformed structures. In this study we have developed a new methodology based on "click chemistry" (CC) to modify the surface of DNA nanostructures with PEG after they are formed. These structures can then be used for in vivo studies and potential applications in the future.
ContributorsSmith, Eric Lynn (Author) / Yan, Hao (Thesis director) / Liu, Yan (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
The prospect of anti-aging or life extension technology is controversial in biogerentology but deemed even by skeptical experts to warrant discussion. I discuss the justifications that the probability of life extension technology being developed in the near future is reasonably high and that this research justifies the time and money it receives. I investigate potential ethical and societal issues anti-aging technology might create. This paper addresses inequality of access, economic cost, changes in quality of life, the role of death in human life, if and how the technology should be regulated and how parties who choose not to undergo treatment can be fairly treated, even when they are a minority.
ContributorsGlesener, Julia Hannah (Author) / McGregor, Joan (Thesis director) / DeSerpa, Allan (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Historical, Philosophical and Religious Studies (Contributor)
Created2015-05
Description
For many pre-health and graduate programs, organic chemistry is often the most difficult prerequisite course that students will take. To alleviate this difficulty, an intelligent tutoring system was developed to provide valuable feedback to practice problems within organic chemistry. This paper focuses on the design and use of an intelligent input parser for nomenclature questions within this system. Students in Dr. Gould's Fall 2014 organic chemistry class used this system and their data was collected to analyze the effectiveness of the input parser. Overall the students' feedback was optimistic and there was a positive relationship between test scores and student use of the system.
ContributorsHusarcik, Edward Andrew (Author) / Gould, Ian (Thesis director) / VanLehn, Kurt (Committee member) / Beerman, Eric (Committee member) / Barrett, The Honors College (Contributor) / Department of Chemistry and Biochemistry (Contributor)
Created2015-05
Description
Currently in synthetic biology only the Las, Lux, and Rhl quorum sensing pathways have been adapted for broad engineering use. Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. While useful, these three quorum sensing pathways exhibit a nontrivial level of crosstalk, hindering robust engineering and leading to unexpected effects in a given design. To address the lack of orthogonality among these three quorum sensing pathways, previous scientists have attempted to perform directed evolution on components of the quorum sensing pathway. While a powerful tool, directed evolution is limited by the subspace that is defined by the protein. For this reason, we take an evolutionary biology approach to identify new orthogonal quorum sensing networks and test these networks for cross-talk with currently-used networks. By charting characteristics of acyl homoserine lactone (AHL) molecules used across quorum sensing pathways in nature, we have identified favorable candidate pathways likely to display orthogonality. These include Aub, Bja, Bra, Cer, Esa, Las, Lux, Rhl, Rpa, and Sin, which we have begun constructing and testing. Our synthetic circuits express GFP in response to a quorum sensing molecule, allowing quantitative measurement of orthogonality between pairs. By determining orthogonal quorum sensing pairs, we hope to identify and adapt novel quorum sensing pathways for robust use in higher-order genetic circuits.
ContributorsMuller, Ryan (Author) / Haynes, Karmella (Thesis director) / Wang, Xiao (Committee member) / Barrett, The Honors College (Contributor) / School of Mathematical and Statistical Sciences (Contributor) / Department of Chemistry and Biochemistry (Contributor) / School of Life Sciences (Contributor)
Created2015-05
Description
Today, the global fashion industry is valued at $450 billion, and considered one of the most important sectors of the global economy (Global Action Through Fashion, 2015). The term fashion means more than just designing apparel or accessories as the industry encompasses jobs from production to inventory management, merchandising, marketing, production, and retail management. The fashion industry is one of the world’s largest markets as it employs over 75 million people and generates $1.7 trillion in revenue annually (Global Action Through Fashion, 2015). It is a dynamic, fast-paced industry that requires constant innovation ideas and strategic planning.
Chloe Bosmeny and Audree López, senior marketing students at W. P. Carey have created a proposal for W. P. Carey School of Business and Herberger Institute for Design and the Arts to join together to create an interdisciplinary resource for students interested in pursuing a career in fashion. There are three recommendations in the thesis: the implementation of a Fashion Merchandising certificate encompassing both W. P. Carey and Herberger curriculum, ASU joining the Fashion Institute of Technology’s 3+1 program for dual degrees in New York City, and lastly, improving professional development and career recruitment for ASU students interested in fashion.
But why fashion at Arizona State University? Throughout college, Bosmeny and López struggled to gain the background, skills and experience needed to understand the fashion industry. They, like many of their peers, felt that without the credentials of a university-sponsored fashion program, they weren't marketable to employers. These challenges drove Bosmeny and López to advocate for more fashion resources at ASU.
Based on support from student surveys, in-depth interviews with industry professionals, feedback from ASU Alumni and input from ASU’s largest fashion organization, The Business of Fashion Club- there is a strong desire for increased fashion programming at ASU. There are currently 266 student theses surrounding the keyword “fashion” from Barrett, the Honors College, but there has not been a direct push from students to implement a program at ASU. This thesis aims to illustrate the important ways such programming will greatly benefit ASU and its stakeholders.
In our thesis we will investigate current ASU opportunities related to fashion, gather information from fashion business professionals, gauge student interest in pursuing careers in fashion, and look to peer and aspirational schools in an effort to better understand fashion career resources nationwide. Our hope is to build a stronger curriculum and more successful resources for students to give them the skillsets needed for a successful career in fashion.
Chloe Bosmeny and Audree López, senior marketing students at W. P. Carey have created a proposal for W. P. Carey School of Business and Herberger Institute for Design and the Arts to join together to create an interdisciplinary resource for students interested in pursuing a career in fashion. There are three recommendations in the thesis: the implementation of a Fashion Merchandising certificate encompassing both W. P. Carey and Herberger curriculum, ASU joining the Fashion Institute of Technology’s 3+1 program for dual degrees in New York City, and lastly, improving professional development and career recruitment for ASU students interested in fashion.
But why fashion at Arizona State University? Throughout college, Bosmeny and López struggled to gain the background, skills and experience needed to understand the fashion industry. They, like many of their peers, felt that without the credentials of a university-sponsored fashion program, they weren't marketable to employers. These challenges drove Bosmeny and López to advocate for more fashion resources at ASU.
Based on support from student surveys, in-depth interviews with industry professionals, feedback from ASU Alumni and input from ASU’s largest fashion organization, The Business of Fashion Club- there is a strong desire for increased fashion programming at ASU. There are currently 266 student theses surrounding the keyword “fashion” from Barrett, the Honors College, but there has not been a direct push from students to implement a program at ASU. This thesis aims to illustrate the important ways such programming will greatly benefit ASU and its stakeholders.
In our thesis we will investigate current ASU opportunities related to fashion, gather information from fashion business professionals, gauge student interest in pursuing careers in fashion, and look to peer and aspirational schools in an effort to better understand fashion career resources nationwide. Our hope is to build a stronger curriculum and more successful resources for students to give them the skillsets needed for a successful career in fashion.
ContributorsLopez, Audree (Co-author) / Bosmeny, Chloe (Co-author) / Ostrom, Amy (Thesis director) / Setlow, Jennifer (Committee member) / Barrett, The Honors College (Contributor) / Department of Marketing (Contributor) / Walter Cronkite School of Journalism and Mass Communication (Contributor) / Herberger Institute for Design and the Arts (Contributor) / W. P. Carey School of Business (Contributor)
Created2015-05